Donkeys’ milk protein fraction investigated by electrophoretic methods and mass spectrometric analysis

Criscione, Andrea; Cunsolo, Vincenzo; Bordonaro, Salvatore; Guastella, Anna Maria; Saletti, Rosaria; Zuccaro, Antonio; D'Urso, G.; Marletta, Donata

doi:10.1016/j.idairyj.2008.10.015

Cited by 55 publications

(36 citation statements)

References 45 publications

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“…In this work we focused our attention on the protein profile of DM studying it under different aspects. The 2-DE map gave a panoramic view on the protein composition of DM: among caseins were found mainly αs1-and β-caseins that showed a considerable heterogeneity due to variable degree of phosphorylation and to the presence of genetic variants as shown also by other authors [28,34,49,50]. A particular attention was provided on the study of the phosphorylation degree of caseins: the phosphate group of caseins may influence on different characteristics such as their digestion and the mineral bioavailability, but also (in particular the phosphoserine residues) may influence the immunoreactivity of caseins.…”

Section: Resultsmentioning

confidence: 66%

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A Proteomic Study on Donkey Milk

Vincenzetti¹,

Amici²

2012

Biochem Anal Biochem

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In children with Cow Milk Protein Allergy (CMPA), when it is not possible to breast feed or to use cow milk, the clinical use of donkey milk is considered since several studies have demonstrated the high similarity of donkey milk compared to human milk.An analysis was performed on donkey milk protein profile by two-dimensional electrophoresis (2-DE) followed by N-terminal sequencing in order to give a panoramic view of the proteins that are present in donkey milk. Furthermore, the interest was focused on the casein fractions and on their phosphorylation degree that may influence the calcium binding ability of caseins. At this purpose experiments on donkey milk casein dephosphorylation have been performed and the dephosphorylated casein fractions have been identified after 2-DE analysis followed by N-terminal sequencing. Among caseins were found mainly αs1-and β-caseins that showed a considerable heterogeneity due to variable degree of phosphorylation and to the presence of genetic variants. Finally, a quantitative determination of some antimicrobial proteins, such as lactoferrin and lactoperoxidase, that could be able to stimulate the development of the neonatal intestine, was performed in donkey milk, with the results being 0.080±0.0035 g/L and 0.11±0.027 mg/L, respectively. From the obtained data is evinced that human and donkey milk contain considerable amounts of lysozyme and lactoferrin but lactoperoxidase is present only in small amounts, confirming the high similarity between donkey and human milk. The present study on donkey milk proteins may be useful to assess the nutritional characteristics of this milk that is used to feed children affected by CMPA, but also may open the possibility of utilizing donkey milk in the general population to benefit subjects with CMPA, such as adults and the elderly.

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Section: Resultsmentioning

confidence: 66%

“…In conclusion, the heterogeneity shown in the whole casein analysis by 2-DE may be due to a variable degree of phosphorylation and to genetic variants of αs1-and β-caseins [32][33][34].…”

Section: Characterization Of Donkey Milk Protein Fraction By Twodimenmentioning

confidence: 91%

A Proteomic Study on Donkey Milk

Vincenzetti¹,

Amici²

2012

Biochem Anal Biochem

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“…β-Lg, absent in human milk, is generally considered to be, together with casein fraction, one of the main causes of CMPA (Restani et al 2009) because it is able to elicit an allergic reaction in sensitive subjects (Uniacke-Lowe et al 2010). In this light, the identification of animals producing milk lacking in β-Lg II protein (Criscione et al 2009) appears promising to solve potential residual cases of reactivity (Wal 2002).…”

Section: β-Lactoglobulinmentioning

confidence: 99%

Whey proteins and their antimicrobial properties in donkey milk: a brief review

Brumini

Criscione

Bordonaro

et al. 2015

Dairy Sci. & Technol.

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Milk is a source of bioactive compounds essential for health and growth of newborns. Donkey milk, rich in lactose and whey proteins, has been proven to be a good breast milk substitute during infancy and adequate nourishment for patients with cow milk protein allergy. Beside, this milk is gaining a growing interest for human nutrition because of some other alleged health benefit. It shows antibacterial activity toward a wide range of Gram-positive and Gram-negative bacteria, stimulates immune system in convalescence, regulates gastrointestinal flora, and prevents inflammatory and autoimmune diseases. As regards its antimicrobial properties, although all the milk components might contribute to this activity, the whey protein fraction of donkey milk is generally believed to play the main role. The aim of this review is to highlight the antimicrobial properties of donkey milk with a special focus on the whey protein fraction. The effects of preservation and processing treatments on whey protein content and antimicrobial activity are also discussed.

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“…Donkey milk may have special therapeutic properties, being recommended to replace cow's milk in cases where cow's milk protein allergy (CMPA) is manifested and for cosmetic purposes for skin treatment (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Nanoliposome Encapsulation with Donkey Milk Bioactive Proteins and Its Possible Application in Dermatology and Cosmetics

Kocic¹,

Stanković²,

Arsić³

2017

Acta Facultatis Medicae Naissensis

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SU M M A RYNanoliposomes represent natural or synthetic nanoparticles made of phospholipids, which may spontaneously aggregate in an aqueous medium. Their use in dermatology and for cosmetic purposes may offer facilitated delivery in skin via enhanced opening of the tight junctions between the epithelial cell monolayers. As far as their use in dermatology is concerned, both transdermal and local application may offer successful release profile. Donkey milk may have special therapeutic properties when used in cosmetology for skin treatment.The aim of our study was to establish encapsulation efficacy of nanoliposome loaded with skimmed donkey milk and to explore the efficiency of encapsulation of different skimmed donkey milk concentrations in nanoliposomes.In our experimental study, it was documented that 1%, 2% and 5% solutions of skimmed donkey milk were almost equally effectively encapsulated, more than 80% in 1% solution of nanoliposomes, while 10% solution of skimmed donkey milk was encapsulated more efficiently, with 88.9% in 1% of nanoliposome solution. Encapsulation can lead to greater efficiency by enabling the use of lower administration doses and preventing the corresponding side effects, which may be the result of higher doses. Skimmed donkey milk is a suitable encapsulation solution.

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Donkeys’ milk protein fraction investigated by electrophoretic methods and mass spectrometric analysis

Cited by 55 publications

References 45 publications

A Proteomic Study on Donkey Milk

A Proteomic Study on Donkey Milk

Whey proteins and their antimicrobial properties in donkey milk: a brief review

Nanoliposome Encapsulation with Donkey Milk Bioactive Proteins and Its Possible Application in Dermatology and Cosmetics

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