DON-induced changes in bone homeostasis in mink dams

Abstract

IntroductionThe aim of the study was to investigate the mechanical and geometric properties as well as bone tissue and mineral density of long bones in mink dams exposed to deoxynivalenol (DON) since one day after mating, throughout gestation (ca. 46 d) and lactation to pelt harvesting.Material and MethodsThirty clinically healthy multiparous minks (Neovison vison) of the standard dark brown type were used. After the mating, the minks were randomly assigned into two equal groups: non-treated control group and DON group fed wheat contaminated naturally with DON at a concentration of 1.1 mg·kg-1 of feed. Results The final body weight and weight and length of the femur did not differ between the groups. However, DON contamination decreased mechanical endurance of the femur. Furthermore, DON reduced the mean relative wall thickness and vertical wall thickness of the femur, while vertical cortical index, midshaft volume, and cross-sectional moment of inertia increased. Finally, DON contamination did not alter bone tissue density, bone mineral density, or bone mineral content, but decreased the values of all investigated structural and material properties.ConclusionDON at applied concentration probably intensified the process of endosteal resorption, which was the main reason for bone wall thinning and the weakening of the whole bone.

Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins

“…Previous results also indicate that FBs affect cell proliferation in the growth plate, as well as the synthesis of proteoglycans in articular cartilage [10,56]. A previous study performed on minks intoxicated with deoxynivalenol has shown that mycotoxins do not alter bone mineral density, but they do shorten bone and intensify the process of endosteal resorption, leading to a thinner bone wall [33,34].…”

“…[30][31][32]. Animal studies have highlighted various other effects of mycotoxins including intensified endosteal resorption, thinning of the bone wall and overall weakening of the whole bone [33,34]. However, no previous studies have looked at the effects of prenatal FB exposure on the development of the skeletal system.…”

Evaluation of Collagen and Elastin Content in Skin of Multiparous Minks Receiving Feed Contaminated with Deoxynivalenol (DON, vomitoxin) with or without Bentonite Supplementation

“…Collagen present in skin tissue is mainly collagen type I: 75%, type III: 22%, and type V: 3%, so the collagen type I to type III ratio is about 2.5 [33,34]. In our previous works, in which we examined the effect of DON and bentonite on bone mechanical properties in minks, we suggested that malnutrition resulting from the presence of mycotoxicosis in the diet could impaired synthesis of collagen type I [35,36]. Moreover, the effect of mycotoxins (DON, Ochratoxin A) on collagen synthesis was previously observed in studies performed cell lines, showing that mycotoxins can influence the expression of fibrous collagens, including collagen type III [37,38].…”

Structural Changes in Trabecular Bone, Cortical Bone and Hyaline Cartilage as Well as Disturbances in Bone Metabolism and Mineralization in an Animal Model of Secondary Osteoporosis in Clostridium perfringens Infection

“…Bone tissue density (BTD) for a 5-cm-long mid-diaphyseal part was performed using an AccuPyc 1330 helium pycnometer (Micromeritics, Inc., Norcross, GA, USA). Before analysis, the samples were first defatted in 2:1 chloroform and methanol mixture for 12 h and then heated in an oven at 105 °C for 24 h to remove bound water and cooled at 25 °C in vacuum desiccators [ 26 ].…”