Domination of an emerging erythromycin-resistant ptxP3 Bordetella pertussis clone in Shanghai, China

Cai, Jiehao; Chen, Mingliang; Liu, Qiyi; Luo, Jiebo; Yuan, Lingyue; Chen, Yanxin; Chen, Min; Zeng, Mei

doi:10.1016/j.ijantimicag.2023.106835

Cited by 8 publications

(8 citation statements)

References 42 publications

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“…In the late 2010s, a novel MRBP strain carrying the ptxP3 allele emerged in mainland China [ 22 , 34 , 35 ]. ptxP3 confers the strain with an increased pertussis toxin-production ability, and the ptxP3 strain is considered to be more virulent in humans than the ptxP1 strain according to a previous study on MSBP [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…ptxP3 -MRBP isolates possess the MT27 or MT28 genotype, which are common genotypes among epidemic MSBP strains worldwide. A rapid shift from ptxP1 - to ptxP3 -MRBP strains has been observed in Shanghai, China, but the underlying cause remains unclear [ 34 ]. Moreover, the relationship between ptxP1 - and ptxP3 -MRBP strains, specifically if the ptxP3 -MRBP strain emerges independently of ptxP1 -MRBP, is unknown.…”

Section: Discussionmentioning

confidence: 99%

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Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Koide,

Uchitani,

Yamaguchi

et al. 2024

PLoS ONE

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The emergence of macrolide-resistant Bordetella pertussis (MRBP) is a significant problem because it reduces treatment options for pertussis and exacerbates the severity and spread of the disease. MRBP has been widely prevalent in mainland China since the 2010s and has been sporadically detected in other Asian countries. In Japan, two MRBP clinical strains were first isolated in Tokyo and Osaka between June and July 2018. The isolates BP616 in Osaka and BP625 in Tokyo harbored the same virulence-associated allelic genes (including ptxP1, ptxA1, prn1, fim3A, and fhaB3) and MT195 genotype and exhibited similar antimicrobial susceptibility profiles. However, despite their simultaneous occurrence, a distinguishable epidemiological link between these isolates could not be established. To gain further insight into the genetic relationship between these isolates in this study, we performed whole-genome analyses. Phylogenetic analysis based on genome-wide single-nucleotide polymorphisms revealed that the isolates belonged to one of the three clades of Chinese MRBP isolates, but there were 11 single-nucleotide polymorphism differences between BP616 and BP625. Genome structure analysis revealed two large inversions (202 and 523 kbp) and one small transposition (3.8 kbp) between the genomes. These findings indicate that the two Japanese MRBP isolates are closely related to Chinese MRBP isolates but are genomically distinct, suggesting that they were introduced into Japan from mainland China through different transmission routes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Koide,

Uchitani,

Yamaguchi

et al. 2024

PLoS ONE

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“…Recent studies have also identified ptxP3 (MGT2 ST2) isolates in China that carry the macrolide resistance conferring mutation [ 17 , 18 ]. Two such isolates are present in the MGT database and were both located within the globally distributed MGT3 ST7 with different MGT4 types.…”

Section: Discussionmentioning

confidence: 99%

“…B. pertussis may also be under selective pressure from macrolide antibiotics used for treatment of pertussis such as erythromycin or azithromycin [ 13 ]. Macrolide-resistant strains of B. pertussis have recently emerged from the genetic background of ptxP1 strains in China [ 14–16 ] and very recently from the ptxP3 background [ 17 , 18 ]. Although only a small number of macrolide-resistant B. pertussis isolates have been reported in other countries, monitoring of the international spread of macrolide-resistant strains is of paramount importance [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic epidemiology and multilevel genome typing of Bordetella pertussis

Payne

et al. 2023

Emerging Microbes & Infections

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Bordetella pertussis causes pertussis (or whooping cough), a severe respiratory infectious disease in infants, although it can be prevented by whole cell and acellular vaccines. The recent pertussis resurgence in industrialised countries is partly attributed to pathogen adaptation to vaccines, while emergence of antimicrobial resistance, specifically to macrolides in China, has become a concern. Surveillance of current circulating and emerging strains is therefore vital to understand the risks they pose to public health. Although the use of genomics-based typing is increasing a genomic nomenclature for this pathogen has not been well established. Here, we implemented the multilevel genome typing (MGT) system for B. pertussis with five levels of resolution, which provide targeted typing of relevant lineages and discrimination of closely related strains at the finest scale. The lower resolution levels (MGT2 and MGT3) describe the distribution of major vaccine antigen alleles including ptxP , fim3, fhaB and prn, as well as temporal and spatial trends within the B. pertussis global population. Mid-resolution levels (MGT3 and MGT4) enable typing of antibiotic-resistant lineages and Prn deficient lineages within the ptxP3 clade. The high-resolution level (MGT5) can capture finer-scale epidemiology such as outbreaks and local transmission events, with comparable resolution to existing genomic methods of strain-relatedness assessment. The scheme offers stable MGT-type assignments aiding harmonisation of typing and communication between laboratories. The scheme is available at https://mgtdb.unsw.edu.au/pertussis , is regularly updated from global data repositories and accepts public submissions. The MGT scheme provides a comprehensive, robust, and scalable system for global surveillance of B. pertussis .

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“…There has been a noted divergence in the antigens of B. pertussis between the strains circulating in the population and those present in vaccines ( 3 – 4 ). Strains exhibiting a novel promoter for Ptx ( ptxP3 ) have been identified in various countries, including China ( 5 – 7 ). Significant virulence factors such as Prn and Fha, both integral components of B. pertussis vaccines, have shown variations.…”

mentioning

confidence: 99%

Emergence of Erythromycin-Resistant and Pertactin- and Filamentous Hemagglutinin-Deficient <i>Bordetella pertussis</i> Strains — Beijing, China, 2022–2023

Zhou,

Li,

Wang

et al. 2024

China CDC Weekly

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What is already known about this topic? Pertussis has reemerged as a significant public health threat, primarily due to variations in Bordetella pertussis strains, antimicrobial resistance, and vaccine evasion. What is added by this report? All isolated strains were identified as ptxA1/ptxC2/ptxP3/prn150/fim2-1/fim3-1/fhaB1/tcfA2 type and exhibited resistance to erythromycin. Two strains showed a deficiency in Fha, thirty in Prn, and one strain exhibited multiple immunogen deficiencies. What are the implications for public health practice? The emergence and spread of immunogen-deficient strains likely result from prolonged vaccine selection pressure, posing challenges to the efficacy of pertussis vaccines. Additionally, the ongoing dissemination of ptxP3 strains with high-level macrolide resistance presents a significant obstacle to clinical treatment strategies.

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Domination of an emerging erythromycin-resistant ptxP3 Bordetella pertussis clone in Shanghai, China

Cited by 8 publications

References 42 publications

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Genomic epidemiology and multilevel genome typing of Bordetella pertussis

Emergence of Erythromycin-Resistant and Pertactin- and Filamentous Hemagglutinin-Deficient <i>Bordetella pertussis</i> Strains — Beijing, China, 2022–2023

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