Dominant follicles development and estradiol‐17β concentrations in non‐ovulating and ovulating post‐partum Bulgarian Murrah buffaloes

Abstract

This study was designed to evaluate the dominant follicles development and the estradiol-17β concentrations in non-ovulating and ovulating post-partum buffaloes. Sixteen Bulgarian Murrah buffaloes were submitted to transrectal ultrasonographic examination from the 1st post-partum day until day 50, 3 days apart. The follicular diameter of the different categories of follicles and the ovulations was recorded. The animals were allocated into two groups: I (n = 6) non-ovulating and II (n = 10) ovulating buffaloes. Serum estradiol-17β concentrations on the days for dominant follicle registration were measured by enzyme-linked immunosorbent assay. The results were statistically processed by analysis of variance, non-parametric and correlation analysis. The mean intervals between calving and first dominant follicle detection differed significantly (p < .05) among the groups (19.5 ± 6.2 vs. 13.8 ± 5.1 days), while the mean intervals between registered dominant follicles from two successive waves were comparable. The mean follicular diameters for the same category follicles in both groups were similar. Different estradiol-17β concentrations (p < .05) for the first dominant follicle between non-ovulating (23.5 ± 7.0 pg/ml) and ovulating (33.3 ± 8.4 pg/ml) buffaloes were determined. The cumulative percentages of buffaloes with firstly detected dominant follicle and ovulating animals correlated positively (r ≥ .84; p < .05) to post-partum days. In conclusion, non-ovulating and ovulating post-partum Bulgarian Murrah buffaloes showed differences in the development of the first dominant follicle and estradiol-17β concentrations during the time of dominant follicles detection.

Effect of Hormonal Treatment during Early Postpartum Period on Uterine Involution, Steroid Hormone Levels and Ovarian Activity in Bulgarian Murrah Buffaloes

“…A continued anovulatory periods after a short estrus cycle, more than three weeks have been described by Khattab et al, (1990) and Ghoneim et al, (1999). Yotov et al, (2015) stated to differences in the development of the first dominant follicle and estradiol-17β concentrations during the time of dominant follicles formation in ovulating and nonovulating buffaloes. According to (El-Wishy, 2007a) the start of ovarian activity after calving could be determined form reproductive potential of different buffalo breeds.…”