Dominance of zoonotic genotype D of Enterocytozoon bieneusi in bamboo rats (Rhizomys sinensis)

Wang, Haiyan; Liu, Qiang; Jiang, Xiumei; Zhang, Ying; Zhao, Aiyun; Cui, Zhaohui; Li, Dongfang; Qi, Meng; Zhang, Longxian

doi:10.1016/j.meegid.2019.04.025

Cited by 32 publications

(32 citation statements)

References 31 publications

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“…For guinea pigs, only one survey conducted in Peru identified genotype peru16 from household guinea pigs (Table 3) [3], and this genotype was not detected in the present study. In this study, genotype D was the most prevalent genotype in pet fancy rats, which is consistent with two previous reports (85.7% and 89.5%) from wild rats [24,34], as well as other rodents such as pet red-bellied tree squirrels (Callosciurus erythraeus) (75.0%), pet red squirrels (Sciurus vulgaris) (44.3%), and domestic bamboo rats (Rhizomys sinensis) (77.3%) [6,7,26]. Genotype D is considered an important zoonotic genotype worldwide [17].…”

Section: Resultssupporting

confidence: 92%

“…In China, genotype D has been identified in immunocompromized patients and in children with diarrhea [18,[27][28][29]32]. Genotype D has also been identified in a wide range of animal hosts in China, including non-human primates, rodents (mice, rats, squirrels, chipmunks, chinchillas, and bamboo rats), other mammals (pigs, cattle, sheep, goats, alpacas, horses, donkeys, rabbits, dogs, cats, foxes, deer, takins, minks, raccoon dogs, raccoons, lions, and hippos), and birds, as well as in water samples [6,8,9,11,17,24,26,30,31,33,34].…”

Section: Resultsmentioning

confidence: 99%

“…Genotyping based on the internal transcribed spacer (ITS) region of the rRNA gene has identified 11 major phylogenetic groups and more than 470 genotypes of E. bieneusi from various hosts [17]. To date, more than 60 E. bieneusi genotypes have been identified in rodents worldwide [6,8,12,17,24,26,30,34]. For rats (Rattus spp.…”

Section: Introductionmentioning

confidence: 99%

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First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Wang

Zhao

et al. 2020

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Enterocytozoon bieneusi, an obligate intracellular microsporidian parasite, can infect humans and a wide variety of animals worldwide. However, information on the prevalence and molecular characterization of E. bieneusi in pet rats and guinea pigs is lacking. In this study, 325 fecal samples were collected from 152 pet fancy rats and 173 pet guinea pigs purchased from pet shops in Henan and Shandong provinces. The prevalence of E. bieneusi was 11.2% (17/152) in pet fancy rats and 20.2% (35/173) in pet guinea pigs. Genotypes D (n = 12), Peru11 (n = 3), S7 (n = 1) and SCC-2 (n = 1) were identified in pet fancy rats, and genotype S7 (n = 30) and a novel genotype PGP (n = 5) were identified in pet guinea pigs. The ITS sequence and its phylogenetic analysis showed that the novel genotype PGP was distinctly different; it exhibited less than 50% similarity to the reference sequences, and did not cluster with any of the known E. bieneusi genotype groups, forming a unique branch between groups 6 and 7. These data suggest that this is a new E. bieneusi genotype group. This is the first report of E. bieneusi infection in pet fancy rats and pet guinea pigs worldwide. The identification of zoonotic genotypes D, Peru11, and S7 suggests that pet fancy rats and guinea pigs can be potential sources of human microsporidiosis.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

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First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Wang

Zhao

et al. 2020

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“…To the best of our knowledge, this is the rst report of E. bieneusi infections in coypus in China. In the present study, the infection rate of E. bieneusi was 41.2% in coypus, which is higher than the infection rate of E. bieneusi reported in brown rats (7.9%) [8], bamboo rats (5.1%) [15], experimental brown rats (4.8%) [16], commensal rodents (mouse and brown rat) (4.0%) [14], pet chinchillas (3.6%) [17] and red squirrels (19.4%) [18] in China. In addition, lower infection rates were also reported in wild house mice (10.7%) from a hybrid zone across the Czech Republic-Germany border [19], and beavers (15.3%) and muskrats (8.4%) from USA [20].…”

Section: Discussioncontrasting

confidence: 76%

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

Cao

Wang³

et al. 2020

Preprint

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Background: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. Coypus (Myocastor coypus) are semi-aquatic rodents, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown.Methods: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene.Results: E. bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes: CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7) and a novel genotype named CNCP1 (n = 1). Conclusions: The rare genotype CHN4 was the most common one in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. This is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be potential infection source to humans.

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“…Genotype D was found in four bears from Fujian, and this genotype is responsible for most human infections and identified in ≥40 countries [4]. It has been isolated from at least 15 species of animals and some water bodies, and is considered to harbor the highest potential for zoonotic transmission [9,46]. The occurrence of E. bieneusi genotype D in bears suggests its significant zoonotic potential and threat to human health.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and genotypic identification of Cryptosporidium spp. and Enterocytozoon bieneusi in captive Asiatic black bears (Ursus thibetanus) in Heilongjiang and Fujian provinces of China

et al. 2020

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Background: Cryptosporidium and Enterocytozoon bieneusi are two important pathogens with zoonotic potential that cause enteric infections in a wide range of hosts, including humans. Both are transmitted from animals to humans by direct contact or through contaminated equipment. Bears are frequently found in Chinese zoos as ornamental animals as well as farmed as commercial animals, and are therefore in close contact with zoo-or farmkeepers, but the prevalence and zoonotic potential of Cryptosporidium and E. bieneusi in bears is poorly understood. In this study, we aimed to provide data on the occurrence and genetic diversity of Cryptosporidium and E. bieneusi in Asiatic black bears from Heilongjiang and Fujian, China. From May 2015 to December 2017, 218 fresh fecal specimens were collected from captive Asiatic black bears in Heilongjiang (n = 36) and Fujian (n = 182), China. Cryptosporidium and E. bieneusi were examined by PCR amplification of the partial small subunit of ribosomal DNA (SSU rDNA) and the internal transcribed spacer (ITS) region of rDNA, respectively. C. andersoni-positive isolates were subtyped through PCR analysis of the four minisatellite/microsatellite (MS1, MS2, MS3 and MS16) loci. Results: The overall prevalence of Cryptosporidium and E. bieneusi were 2.4% (4/218) and 6.4% (14/218), respectively, with 2.8% (1/36) and 22.2% (8/36) in the Heilongjiang Province, and 1.6% (3/182) and 3.3% (6/182) in the Fujian Province. Sequence analysis confirmed the presence of Cryptosporidium species: C. andersoni (n = 3) and a genotype termed Cryptosporidium rat genotype IV (n = 1). All three identified C. andersoni belonged to the MLST subtype A4, A4, A4, A1. Two known E. bieneusi genotypes D (n = 4) and SC02 (n = 10) were identified, both of which belong to zoonotic Group 1. Conclusions: This is the first report of C. andersoni and Cryptosporidium rat genotype IV in bears. The discovery of the zoonotic potential of E. bieneusi genotype D in bears highlights its significant zoonotic potential and potential threat to human health.

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Dominance of zoonotic genotype D of Enterocytozoon bieneusi in bamboo rats (Rhizomys sinensis)

Cited by 32 publications

References 31 publications

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

Prevalence and genotypic identification of Cryptosporidium spp. and Enterocytozoon bieneusi in captive Asiatic black bears (Ursus thibetanus) in Heilongjiang and Fujian provinces of China

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