Dominance of the Beijing genotype among XDR Mycobacterium tuberculosis strains in Russia

Vyazovaya, Anna; Mokrousov, I. V.; Zhuravlev, Viacheslav; Solovieva, Natalia; Otten, Tatiana; Vishnevsky, Boris; Narvskaya, Olga

doi:10.1016/j.ijmyco.2014.10.040

Cited by 5 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The standard chemotherapy regimen, therefore, might perform poorly and on average not well suited for the observed extent of drug resistance in our region of Russia. A further complicating factor might be that majority of M. tuberculosis in Russia belongs to the Beijing strain (around 70%) that has increased virulence, extremely high transmissibility, and association with MDR phenotype (Afanas'ev et al, 2011;Vyazovaya et al, 2015). According to our findings, it is evident that that current chemotherapy regimen must be revised to meet with the observed distribution of drug-resistant strains in the region.…”

Section: Discussionmentioning

confidence: 72%

Treatment efficacy of drug-resistant tuberculosis in Bashkortostan, Russia: A retrospective cohort study

Yunusbaeva

Borodina

Alekseev

et al. 2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Background: Russia, together with other former Soviet Union countries, is characterized by one of the highest burdens of drug-resistant tuberculosis. Published data on the drug-resistant tuberculosis for these countries are limited, and it is not clear whether current treatment regimens remain effective against constantly evolving drug-resistant strains. Objectives: The aim of the study was to evaluate treatment efficacy of patients with multidrug-resistant (MDR), extensively drug-resistant (XDR) and drug-susceptible (DSTB) tuberculosis in the most populous region of Russia (Bashkortostan) that borders with Central Asia. Methods: A retrospective cohort study was performed on 436 patients with pulmonary tuberculosis who were enrolled between January 1, 2016, and February 28, 2018, and received treatment according to WHO recommendations. Altogether, 369 patients completed the full course of chemotherapy. Clinical characteristics and treatment outcomes of DSTB, MDR, and XDR-TB patients were analyzed. Results: Of 436 patients, 169 (39%) had XDR-TB, 94 (22%) had MDR-TB and 173 (40%) had DSTB. Half of the MDR-TB patients (44%) and 82% of XDR-TB patients failed treatment. Patients with DSTB had unexpectedly poor treatment efficacy: only 67% had treatment success. We found that most of the MDR isolates from our patients were resistant to all first-line drugs, and a majority of the XDR isolates were resistant to more than 6-7 anti-TB drugs. While this can explain poor treatment efficacy in drug-resistant cases, causes of poor treatment efficacy in DSTB patients remain unclear. Finally, a considerable fraction (46%) of newly diagnosed patients had MDR-TB (27%) and XDR-TB (19%), suggesting that drug-resistant Mtb is being transmitted in the general population. To our best knowledge, this study is the first one to report XDR-TB prevalence in Russia in recent years (2016)(2017)(2018). Conclusions: MDR and XDR-TB became more common in recent years and treatment efficacy is declining at the face of more extensive drug resistance. There is evidence for the transmission of resistant strains in the general population, which calls for urgent changes not only in clinical practice but also in measures to prevent spread in the general population.

show abstract

Section: Discussionmentioning

confidence: 72%

Treatment efficacy of drug-resistant tuberculosis in Bashkortostan, Russia: A retrospective cohort study

Yunusbaeva

Borodina

Alekseev

et al. 2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…More recent data indicated that the XDR M. tuberculosis strain population in northwestern Russia is heavily dominated by Beijing genotype isolates (Vyazovaya et al, 2015). In Pskov oblast, which is located in northwestern Russia on the Russian/Latvian border, the Beijing genotype was the most prevalent followed by LAM, T, Haarlem, Ural, and Manu2 families .…”

Section: Discussionmentioning

confidence: 99%

Analysis of Mycobacterium tuberculosis genetic lineages circulating in Riga and Riga region, Latvia, isolated between 2008 and 2012

Pole

Trofimova

Norvaiša

et al. 2020

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Although the number of new tuberculosis (TB) cases registered per year has decreased by 3-fold between 2001 and 2017 in Latvia, the TB incidence and rates of multidrug resistant TB in this Baltic country remain substantially higher than in most other European countries. Molecular typing methods of Mycobacterium tuberculosis (MTB) play an important role both in clinical studies of the disease and the epidemiological investigations, allowing to describe and characterize the pathogen's population structure and spread of particular genotypes. Aim of this study was to examine the prevalence of MTB lineages in Riga and Riga region of Latvia within a five-year period (2008 -2012), and to evaluate the discriminatory power (DP) of spoligotyping, standard 24locus MIRU-VNTR and IS6110-RFLP methods in this setting. The results showed that the main MTB spoligotype families were Beijing (25.3%) and LAM (24.3%), followed by T (22.1%), Ural (11.2%), Haarlem (6.6%) and X superfamily (3.4%). This distribution remained stable over the five consecutive years. 67.6% of MTB isolates were pan-susceptible, and 32.4% were resistant to any drug; multi-drug resistance was found in 5.8% of MTB strains, and 7.6% of MTB isolates were extensively drugresistant. Drug resistance was associated with SIT1, SIT283 and SIT42 genotypes, while SIT1 and SIT42 were overrepresented among multi drugresistant MTB strains. Overall, DP of spoligotyping method alone was 0.8953, while DP of both 24-locus MIRU-VNTR analysis and IS6110 RFLP was higher (DP=0.9846 and 0.9927, respectively), mainly due to the improvement of the resolution for the Beijing strains. In conclusion, this work represents the first comprehensive molecular epidemiological description of TB in Latvia, highlighting the high genetic diversity of MTB strains circulating in Riga and Riga region. In combination with detailed epidemiological data this approach was helpful for the in-depth understanding of epidemiological processes in settings where the Next-Gen sequencing is not available as a routine method.

show abstract

“…In other parts of Russia it was somewhat lower, 46.9 % in Ural [ 36 ] and 34 % in Siberia [ 37 ]. With further evolution of the TB epidemic in Russia, the MDR phenotype is evolving to XDR, and consequently, this association is repeated for XDR [ 38 , 39 ]. One can hardly expect an improvement of the situation in the country in view of the (i) unchanged M. tuberculosis population structure with increasing prevalence of the drug resistant variants and (ii) unchanged (insufficient) TB control measures.…”

Section: Discussionmentioning

confidence: 99%

Trends in molecular epidemiology of drug-resistant tuberculosis in Republic of Karelia, Russian Federation

et al. 2015

View full text Add to dashboard Cite

BackgroundRussian Republic of Karelia is located at the Russian-Finnish border. It contains most of the historical Karelia land inhabited with autochthonous Karels and more recently migrated Russians. Although tuberculosis (TB) incidence in Karelia is decreasing, it remains high (45.8/100 000 in 2014) with the rate of multi-drug resistance (MDR) among newly diagnosed TB patients reaching 46.5 %. The study aimed to genetically characterize Mycobacterium tuberculosis isolates obtained at different time points from TB patients from Karelia to gain insight into the phylogeographic specificity of the circulating genotypes and to assess trends in evolution of drug resistant subpopulations.MethodsThe sample included 150 M. tuberculosis isolates: 78 isolated in 2013–2014 (“new” collection) and 72 isolated in 2006 (“old” collection). Drug susceptibility testing was done by the method of absolute concentrations. Spoligotyping was used to test genotype-specific markers of a Latin-American-Mediterranean (LAM) family and its sublineages as well as a Beijing B0/W148-cluster.ResultsThe largest spoligotypes were SIT1 (Beijing family, n = 42) and SIT40 (T family, n = 5). Beijing family was the largest (n = 43) followed by T (n = 11), Ural (n = 10) and LAM (n = 8). Successful Russian clone, Beijing В0/W148, was identified in 15 (34.9 %) of 43 Beijing isolates; all В0/W148 isolates were drug-resistant. Seven of 8 LAM isolates belonged to the RD115/LAM-RUS branch, 1 - to the LAM RD174/RD-Rio sublineage. MDR was found in Beijing (32/43), Ural (3/10), and LAM (3/8). In contrast, all T isolates were pansusceptible. Comparison of drug resistant subgroups of the new and old collections showed an increasing prevalence of the B0/W148 clonal cluster, from 18.0 % (mainly polyresistant) in 2006 to 32.6 % in 2014 (mainly MDR and pre-XDR). The West–east increasing gradient is observed for the Ural genotype that may be defined a ‘Russian’ strain. In contrast, the spoligotype SIT40 of the T family appears to be a historical Karelian strain.ConclusionsCirculation of the MDR M. tuberculosis isolates of the Beijing genotype and its B0/W148 cluster continues to critically influence the current situation with the MDR-TB control in northwestern Russia including the Republic of Karelia. Revealed phylogeographic patterns of some genotypes reflect a complex demographic history of Karelia within the course of the 20th century.

show abstract

Dominance of the Beijing genotype among XDR Mycobacterium tuberculosis strains in Russia

Cited by 5 publications

References 0 publications

Treatment efficacy of drug-resistant tuberculosis in Bashkortostan, Russia: A retrospective cohort study

Treatment efficacy of drug-resistant tuberculosis in Bashkortostan, Russia: A retrospective cohort study

Analysis of Mycobacterium tuberculosis genetic lineages circulating in Riga and Riga region, Latvia, isolated between 2008 and 2012

Trends in molecular epidemiology of drug-resistant tuberculosis in Republic of Karelia, Russian Federation

Contact Info

Product

Resources

About