Dominance and succession of Microcystis genotypes and morphotypes in Lake Taihu, a large and shallow freshwater lake in China

Yang, Liuyan; Xu, Yao; Wang, Zhongjie; Xiao, Peng; Yu, Ge; Wang, Guoxiang; Li, Renhui

doi:10.1016/j.envpol.2016.05.021

Cited by 33 publications

(15 citation statements)

References 49 publications

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“…NMDS and PERMANOVA analyses were performed using MicrobiomeAnalyst platform (http://www.microbiomeanalyst.ca/) in order to investigate the influence of the species, the sampling localities and of the production of MCs, described as the variables, on the global metabolite distribution of the global metabolome observed on ESI-Qq-TOF for the 24 strains. Using the whole MSMS data (converted in mgf format) obtained for the 24 strains taken together, a molecular network was created using the online workflow at Global Natural Products Social molecular networking (GNPS) (http://gnps.ucsd.edu) (Wang et al, 2016). The data were then clustered with MS-Cluster with a parent mass tolerance of 1.0 Da and an MS/MS fragment ion tolerance of 0.5 Da to create consensus spectra.…”

Section: Metabolome Biomass Extraction and Analysis By Mass Spectrometrymentioning

confidence: 99%

“…Seven of these clusters encode enzymes for the biosynthesis of known metabolites (microcystins, aeruginosins, cyanopeptolins, microginins, anabaenopeptins, cyanobactins and microviridins), whereas the six remaining clusters encode enzymes for the biosynthesis of still unidentified products. However, the relationship between cyanobacterial biomass and metabolite concentrations in the environment appears neither systematic nor linear (Briand et al 2002;Liu et al, 2016). Indeed, the production of metabolites, such as microcystins, by Microcystis blooms, depends not only on cyanobacterial biomass, but also on the ratio between potentially producing and non-producing genotypes within the population (Via-Ordorika et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity

Marie

Manach

Duval

et al. 2018

Preprint

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Cyanobacteria are photosynthetic prokaryotes that are able to synthetize a wild rang of secondary metabolites exhibiting noticeable bioactivity, comprising toxicity. Microcystis represents one of the most common cyanobacteria taxa constituting the intensive blooms that arise nowadays in freshwater ecosystems worldwide. They produce numerous cyanotoxins (toxic metabolites), which are potentially harmful to Human health and aquatic organisms. In order to better understand the variations in cyanotoxins production between clones of the same blooms, we investigate the diversity of several Microcystis strains isolated from different freshwater bloom-forming populations from various geographical area. Twenty-four clonal strains were compared by genotyping with 16S-ITS fragment sequencing and metabolites chemotyping using LC ESI-qTOF mass spectrometry. While, genotyping can only discriminate between the different species, the global metabolomes reveal clear discriminant molecular profiles between strains, which can be clustered primarily according to their global metabolite content, then to their genotype, and finally to their sampling localities. A global molecular network generated from MS/MS fractionation patterns of the various metabolite detected in all strains performed with GNPS tool highlight the production of a wide set of chemically diverse metabolites, comprising only few microcystins, but many aeruginosins, cyanopeptolins and microginins, along with a large set of unknown molecules that still remain to be investigated and characterized at their structure as well as at their potential bioactivity or toxicity levels. Figure 1: Microcystis spp. General view of a representative intense Microcystis sp. bloom in a recreational pound (Champs-sur-Marne) (A). Macrograph of Microcystis colonies at surface water (B). Example of 15-mL vessels containing the monoclonal strains of Microcystis spp. maintained in the Paris' Museum Collection (PMC) of cyanobacteria (MNHN, Paris) (C). Example of micrograph of the isolated monoclonal culture of the Microcystis aeruginosa, where scale bare represents 10 µm (D). Representative picture of Microcystis aeruginosa cell from PMC 156.02 strain (here in division) under transmission electron microscope, where scale bare represents 0.5 µm (E). General structures of various cyanobacterial metabolites belonging to the microcystin, anabaenopeptin, microginin microviridin, aeruginosin and oscillatorin families (F).Microcystis wesenbergii PMC 672.10 84 Microcystis wesenbergii PMC 671.10 Microcystis wesenbergii PMC 673.10 Microcystis wesenbergii PMC 674.10 Microcystis wesenbergii NIES111 (3551457) (AB015388) Microcystis wesenbergii TAC52 (3551459) (AB015390) Microcystis sp. PMC 807.12 Microcystis viridis PMC 566.08 88 Microcystis wesenbergii NIES44 (3551456) (AB015387) Microcystis viridis TAC17 (3551467) (AB015398) Microcystis viridis W191 (295983850) (HM017091) Microcystis viridis W18 (295983846) (HM017087) Microcystis aeruginosa PMC 265.05 Microcystis sp. PMC 816.12 Microcystis aeruginosa PMC...

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Section: Metabolome Biomass Extraction and Analysis By Mass Spectrometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity

Marie

Manach

Duval

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, each of above genera contains both toxin-producing and nontoxin-producing species. Microcystis is the most frequently reported bloom-forming cyanobacterium, which often occurs as dense blooms in summer and autumn [ 9 , 10 , 11 ]. In subtropical and temperate lakes, Dolichospermum and Aphanizomenon usually become dominant when the abundance of Microcystis decreases in winter and spring [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have revealed that populations of bloom-forming cyanobacteria are highly diverse [ 9 , 24 , 25 ]. During a bloom, dynamic changes occur in the genotypic composition of a cyanobacterial population [ 11 , 24 , 25 ]. In a previous study, the composition of Microcystis genotypes and morphotypes varied periodically and were significantly affected by water temperature [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…During a bloom, dynamic changes occur in the genotypic composition of a cyanobacterial population [ 11 , 24 , 25 ]. In a previous study, the composition of Microcystis genotypes and morphotypes varied periodically and were significantly affected by water temperature [ 11 ]. The conditions favorable for Microcystis growth are more beneficial for nontoxin-producing genotypes, whereas unfavorable growth conditions can increase the relative abundance of toxin-producing genotypes [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Revealing Cryptic Changes of Cyanobacterial Community Structure in Two Eutrophic Lakes Using eDNA Sequencing

Jiang

Xiao

et al. 2020

IJERPH

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Harmful cyanobacterial blooms pose a risk to human health worldwide. To enhance understanding on the bloom-forming mechanism, the spatiotemporal changes in cyanobacterial diversity and composition in two eutrophic lakes (Erhai Lake and Lushui Reservoir) of China were investigated from 2010 to 2011 by high-throughput sequencing of environmental DNA. For each sample, 118 to 260 cpcBA-IGS operational taxonomic units (OTUs) were obtained. Fifty-two abundant OTUs were identified, which made up 95.2% of the total sequences and were clustered into nine cyanobacterial groups. Although the cyanobacterial communities of both lakes were mainly dominated by Microcystis, Erhai Lake had a higher cyanobacterial diversity. The abundance of mixed Nostocales species was lower than that of Microcystis, whereas Phormidium and Synechococcus were opportunistically dominant. The correlation between the occurrence frequency and relative abundance of OTUs was poorly fitted by the Sloan neutral model. Deterministic processes such as phosphorus availability were shown to have significant effects on the cyanobacterial community structure in Erhai Lake. In summary, the Microcystis-dominated cyanobacterial community was mainly affected by the deterministic process. Opportunistically dominant species have the potential to replace Microcystis and form blooms in eutrophic lakes, indicating the necessity to monitor these species for drinking water safety.

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Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster

Bi,

Wei,

Wang

et al. 2024

Curr Microbiol

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Dominance and succession of Microcystis genotypes and morphotypes in Lake Taihu, a large and shallow freshwater lake in China

Cited by 33 publications

References 49 publications

Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity

Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity

Revealing Cryptic Changes of Cyanobacterial Community Structure in Two Eutrophic Lakes Using eDNA Sequencing

Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster

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