Domains of Tra1 Important for Activator Recruitment and Transcription Coactivator Functions of SAGA and NuA4 Complexes

Knutson, Bruce A.; Hahn, Steven

doi:10.1128/mcb.00687-10

Cited by 80 publications

(124 citation statements)

References 92 publications

(112 reference statements)

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“…Moreover, the interaction of Gcn4 with SAGA in cell extracts was not impaired by the Taf9 CRD mutation. Tra1 and Taf12, two SAGA subunits previously shown to interact with the Gcn4 activation domain (51,63,69), are efficiently coimmunoprecipitated in a complex with Spt7 (Fig. 5D), and the same subunits were also pulled down by Gcn4 (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies have shown that SAGA interaction with the Gcn4 activation domain is mediated by Tra1 (51, 63) and Taf12 subunits (48,63). Moreover, these subunits are also required for transcriptional activation of target genes by Gcn4, thereby providing physical and functional interaction of SAGA subunits with Gcn4.…”

Section: Resultsmentioning

confidence: 95%

“…SAGA recruitment to the UAS is through direct activator interactions (51,63,69,70). TFIID recruitment to promoters is mediated via Rap1 (2, 60), as well as through core promoter interactions (19).…”

Section: Discussionmentioning

confidence: 99%

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The TAF9 C-Terminal Conserved Region Domain Is Required for SAGA and TFIID Promoter Occupancy To Promote Transcriptional Activation

Saint

Sawhney

Sinha

et al. 2014

Molecular and Cellular Biology

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A common function of the TFIID and SAGA complexes, which are recruited by transcriptional activators, is to deliver TBP to promoters to stimulate transcription. Neither the relative contributions of the five shared TBP-associated factor (TAF) subunits in TFIID and SAGA nor the requirement for different domains in shared TAFs for transcriptional activation is well understood. In this study, we uncovered the essential requirement for the highly conserved C-terminal region (CRD) of Taf9, a shared TAF, for transcriptional activation in yeast. Transcriptome profiling performed under Gcn4-activating conditions showed that the Taf9 CRD is required for induced expression of ϳ9% of the yeast genome. The CRD was not essential for the Taf9-Taf6 interaction, TFIID or SAGA integrity, or Gcn4 interaction with SAGA in cell extracts. Microarray profiling of a SAGA mutant (spt20⌬) yielded a common set of genes induced by Spt20 and the Taf9 CRD. Chromatin immunoprecipitation (ChIP) assays showed that, although the Taf9 CRD mutation did not impair Gcn4 occupancy, the occupancies of TFIID, SAGA, and the preinitiation complex were severely impaired at several promoters. These results suggest a crucial role for the Taf9 CRD in genome-wide transcription and highlight the importance of conserved domains, other than histone fold domains, as a common determinant for TFIID and SAGA functions.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 95%

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The TAF9 C-Terminal Conserved Region Domain Is Required for SAGA and TFIID Promoter Occupancy To Promote Transcriptional Activation

Saint

Sawhney

Sinha

et al. 2014

Molecular and Cellular Biology

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“…As mentioned above, NuA4 is a multisubunit protein complex and is essential for acetylation of histones H4, H2A, and H2A.Z (4-7). NuA4 is targeted to the UAS of the promoter of the target gene by a transcriptional activator (10,11,14,17,46,70). At the ribosomal protein genes, NuA4 is recruited by the activator Rap1p, and such targeted recruitment is essential for transcriptional initiation (10,14,17,70).…”

Section: Resultsmentioning

confidence: 99%

Eaf1p Is Required for Recruitment of NuA4 in Targeting TFIID to the Promoters of the Ribosomal Protein Genes for Transcriptional Initiation In Vivo

Uprety

Sen

Bhaumik

2015

Molecular and Cellular Biology

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NuA4 (nucleosome acetyltransferase of H4) promotes transcriptional initiation of TFIID (a complex of TBP and TBP-associated factors [TAFs])-dependent ribosomal protein genes involved in ribosome biogenesis. However, it is not clearly understood how NuA4 regulates the transcription of ribosomal protein genes. Here, we show that NuA4 is recruited to the promoters of ribosomal protein genes, such as RPS5, RPL2B, and RPS11B, for TFIID recruitment to initiate transcription, and the recruitment of NuA4 to these promoters is impaired in the absence of its Eaf1p component. Intriguingly, impaired NuA4 recruitment in a ⌬eaf1 strain depletes recruitment of TFIID (a TAF-dependent form of TBP) but not the TAF-independent form of TBP to the promoters of ribosomal protein genes. However, in the absence of NuA4, SAGA (Spt-Ada-Gcn5-acetyltransferase) is involved in targeting the TAF-independent form of TBP to the promoters of ribosomal protein genes for transcriptional initiation. Thus, NuA4 plays an important role in targeting TFIID to the promoters of ribosomal protein genes for transcriptional initiation in vivo. Such a function is mediated via its targeted histone acetyltransferase activity. In the absence of NuA4, ribosomal protein genes lose TFIID dependency and become SAGA dependent for transcriptional initiation. Collectively, these results provide significant insights into the regulation of ribosomal protein gene expression and, hence, ribosome biogenesis and functions.H istone H4 acetylation plays important roles in the regulation of eukaryotic transcription and other biological processes (1-3). In Saccharomyces cerevisiae, NuA4 (nucleosome acetyltransferase of H4) acetylates histone H4. In addition, NuA4 is involved in acetylation of histones H2A and H2A.Z (4-7). NuA4 is a multisubunit protein complex and is conserved from yeast to humans (Tip60 is the human homologue of yeast NuA4) (8). Like other histone lysine (K) acetyltransferases (KATs), NuA4 is involved in various cellular events, such as transcription, DNA repair, and cell cycle progression (9-27). In addition, NuA4 is proposed to regulate cellular aging and autophagy via acetylation of nonhistone proteins (28-30). Likewise, Tip60 has numerous nonhistone targets involved in various cellular activities (31, 32). In addition, Tip60 has been found to be involved in performing critical functions in DNA repair and stem cell regulation (33-36). Therefore, NuA4 and its human homologue are multifunctional in maintaining normal cellular functions.Esa1p is the catalytic subunit of NuA4 with KAT activity (37, 38). In addition, NuA4 has 12 other subunits (39, 40). These subunits include Tra1p (ATM-related factor), Epl1p (enhancer of polycomb homologue), Arp4p (actin-related protein), Yaf9p (leukemogenic factor ENL/AF9 homologue), Act1p, and 7 Esa1p-associated factors, Eaf1p to Eaf7p. Eaf2p and Eaf4p are also known as Swc4p and Yng2p, respectively. Although Esa1p is the catalytic subunit of NuA4, it cannot acetylate nucleosomal histones on its own but can acetylate naked/f...

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“…Tra1 is also likely responsible for activator recruitment of the NuA4 coactivator. Most of Tra1 is composed of short repeated motifs, including HEAT and TPR repeats, which are N-terminal to the PI3 kinase-like domain (Knutson and Hahn 2011). Systematic mutation of Tra1 has shown that about two-thirds of the protein is essential for growth.…”

Section: Tra1 Has Multiple Functions Within Saga and Nua4mentioning

confidence: 99%

Transcriptional Regulation inSaccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators

2011

Self Cite

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Here we review recent advances in understanding the regulation of mRNA synthesis in Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been conserved in all eukaryotes, and budding yeast has been at the forefront in the discovery and dissection of these conserved mechanisms. Topics covered include upstream activation sequence and promoter structure, transcription factor classification, and examples of regulated transcription factor activity. We also examine advances in understanding the RNA polymerase II transcription machinery, conserved coactivator complexes, transcription activation domains, and the cooperation of these factors in gene regulatory mechanisms. TABLE OF CONTENTS Abstract 705Introduction 706

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Domains of Tra1 Important for Activator Recruitment and Transcription Coactivator Functions of SAGA and NuA4 Complexes

Cited by 80 publications

References 92 publications

The TAF9 C-Terminal Conserved Region Domain Is Required for SAGA and TFIID Promoter Occupancy To Promote Transcriptional Activation

The TAF9 C-Terminal Conserved Region Domain Is Required for SAGA and TFIID Promoter Occupancy To Promote Transcriptional Activation

Eaf1p Is Required for Recruitment of NuA4 in Targeting TFIID to the Promoters of the Ribosomal Protein Genes for Transcriptional Initiation In Vivo

Transcriptional Regulation inSaccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators

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