Domains II and III of Bacillus thuringiensis Cry1Ab toxin remain exposed to the solvent after insertion of part of domain I into the membrane.

Abstract

PAGE 19111:An error has been detected in the methodology description of "Insertion of Cry1Ab Mutant Toxin into the BBMV." The samples used in Western blot assays were not boiled. Instead, they were incubated for 5 min at 37°C before loading into 10% SDS-PAGE. A new supplemental Fig. S1 is presented. FIGURE S1. Western blot analysis of membrane associated proteins. Mutant proteins were incubated with BBMV at pH 9.0. Toxin proteins inserted into the BBMV were recovered by centrifugation. Pellets were suspended in Laemmli sample buffer, incubated 5 min at 37°C, loaded into 10% SDS-PAGE, and analyzed by Western blot using polyclonal anti-Cry1Ab antibody and a secondary goat anti-rabbit HRP antibody. The membrane pellet showed that all proteins form oligomeric structures when incubated with Manduca sexta BBMV.