Domain swapping reveals functional modularity present in the decay-accelerating factor (CD55)

Panwar, Hemendra Singh; Ojha, Hina; Ghosh, Payel; Raut, Sunil; Sahu, Arvind

doi:10.1016/j.imbio.2016.06.128

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stepwise loop insertion strategy (StLois) identifies target regions through structural and functional analysis of the corresponding enzymes, effectively expanding the residues in the loop regions to provide new structures of the enzyme active site for new catalytic properties (Hoque et al, 2020). Domain swapping helps reveal structural and functional information on important regulators, such as β-repressors (Ghosh et al, 2019) and decay accelerators (Panwar et al, 2016).…”

Section: Structure-based Evolutionmentioning

confidence: 99%

Enabling technology and core theory of synthetic biology

Zhang

Liu

Dai

et al. 2023

Sci. China Life Sci.

View full text Add to dashboard Cite

Synthetic biology provides a new paradigm for life science research (“build to learn”) and opens the future journey of biotechnology (“build to use”). Here, we discuss advances of various principles and technologies in the mainstream of the enabling technology of synthetic biology, including synthesis and assembly of a genome, DNA storage, gene editing, molecular evolution and de novo design of function proteins, cell and gene circuit engineering, cell-free synthetic biology, artificial intelligence (AI)-aided synthetic biology, as well as biofoundries. We also introduce the concept of quantitative synthetic biology, which is guiding synthetic biology towards increased accuracy and predictability or the real rational design. We conclude that synthetic biology will establish its disciplinary system with the iterative development of enabling technologies and the maturity of the core theory.

show abstract

Section: Structure-based Evolutionmentioning

confidence: 99%