Domain structure and subunit interactions in the type I DNA methyltransferase M.EcoR124I

Smith, Melanie A.; Read, Christopher M.; Kneale, Geoff

doi:10.1006/jmbi.2001.5123

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…RM type I systems play a role in phage resistance due to digestion of incoming DNA unmethylated in a specific manner [31]. S-proteins are involved in DNA sequence recognition determining the specificity of methylase-endonuclease complexes [32]. The methylase and endonuclease subunits are encoded, respectively by chromosomal genes hsdM and hsdR .…”

Section: Resultsmentioning

confidence: 99%

Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

et al. 2011

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The extrachromosomal gene pool plays a significant role both in evolution and in the environmental adaptation of bacteria. The L. lactis subsp. lactis IL594 strain contains seven plasmids, named pIL1 to pIL7, and is the parental strain of the plasmid-free L. lactis IL1403, which is one of the best characterized lactococcal strains of LAB. Complete nucleotide sequences of pIL1 (6,382 bp), pIL2 (8,277 bp), pIL3 (19,244 bp), pIL4 (48,979), pIL5 (23,395), pIL6 (28,435 bp) and pIL7 (28,546) were established and deposited in the generally accessible database (GeneBank). Nine highly homologous repB-containing replicons, belonging to the lactococcal theta-type replicons, have been identified on the seven plasmids. Moreover, a putative region involved in conjugative plasmid mobilization was found on four plasmids, through identification of the presence of mob genes and/or oriT sequences. Detailed bioinformatic analysis of the plasmid nucleotide sequences provided new insight into the repertoire of plasmid-encoded functions in L. lactis, and indicated that plasmid genes from IL594 strain can be important for L. lactis adaptation to specific environmental conditions (e.g. genes coding for proteins involved in DNA repair or cold shock response) as well as for technological processes (e.g. genes encoding citrate and lactose utilization, oligopeptide transport, restriction-modification system). Moreover, global gene analysis indicated cooperation between plasmid- and chromosome-encoded metabolic pathways.

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Section: Resultsmentioning

confidence: 99%

Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

et al. 2011

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“…In detail, phage DNA usually reveal different methylation patterns than those recognized by innate RM systems. Unmethylated target sequences are significantly susceptible to endonucleolytic attack, resulting in DNA degradation [35]. Such mode of action guarantees that the presence of RM systems limits phage proliferation in the cytoplasm, causing no harm to the cell.…”

Section: Restriction Modification Systemsmentioning

confidence: 99%

“…Such mode of action guarantees that the presence of RM systems limits phage proliferation in the cytoplasm, causing no harm to the cell. RM systems are classified into four groups, based on their molecular structure, co-factor requirements, sequence recognition and cleavage position [34][35][36].…”

Section: Restriction Modification Systemsmentioning

confidence: 99%

“…just after replication), the enzyme will exhibit activity of a methyltransferase, which completes DNA modification. In contrast, if the holoenzyme binds to an unmethylated recognition site, DNA translocation past the DNA-enzyme complex occurs in an ATP-dependent manner [35,38]. In spite of DNA translocation, the enzyme remains bound to the target site.…”

Section: Type I Rmmentioning

confidence: 99%

“…The central domain, located between two TRDs, is responsible for interaction with one HsdM subunit. Other conserved regions located at N and C termini have been proposed to form a split domain, which makes contact with a second HsdM subunit [35,37].…”

Section: Type I Rmmentioning

confidence: 99%

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Lactic Acid Bacteria Resistance to Bacteriophage and Prevention Techniques to Lower Phage Contamination in Dairy Fermentation

Szczepankowska¹,

Górecki²,

Bardowski³

2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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Survey of the year 2001 commercial optical biosensor literature

Rich

Myszka

2002

J of Molecular Recognition

101

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We have assembled references of 700 articles published in 2001 that describe work performed using commercially available optical biosensors. To illustrate the technology's diversity, the citation list is divided into reviews, methods and specific applications, as well as instrument type. We noted marked improvements in the utilization of biosensors and the presentation of kinetic data over previous years. These advances reflect a maturing of the technology, which has become a standard method for characterizing biomolecular interactions.

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Domain structure and subunit interactions in the type I DNA methyltransferase M.EcoR124I

Cited by 8 publications

References 26 publications

Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

Lactic Acid Bacteria Resistance to Bacteriophage and Prevention Techniques to Lower Phage Contamination in Dairy Fermentation

Survey of the year 2001 commercial optical biosensor literature

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