Domain growth dynamics in multicomponent vesicles composed of BSM/DOPC/cholesterol

Liang, Xinyu; Li, Li; Qiu, Feng; Yang, Yuliang

doi:10.1016/j.physa.2010.05.052

Cited by 8 publications

(8 citation statements)

References 35 publications

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“…We find results in good agreement with current theory both for domains that grow primarily by coalescence (for f % 0.3, and for late times of 0.3 % f % 0.7) and for elongated domains (for early times of 0.3 % f % 0.7). Our experiments differ from previous work in that we focus on a regime in which coalescence of domains dominates (14) and in our avoidance of small vesicles, inconstant temperature, substrate interactions, and/or photooxidation (15)(16)(17)19). Our measurements of diffusion coefficients yield 2D membrane viscosities of 3 Â 10 À9 Pa s m, in excellent agreement with literature values.…”

Section: Introductionsupporting

confidence: 81%

“…Work by others (23,24) implies that no net attractive or repulsive interactions exist between unbulged domains because fluctuations in domain boundaries fit normal capillary theory (Tobias Baumgart, personal communication). In 2007, Liang et al (17) reported a z 1/3 for domains smaller than 1 mm and a z 1 for domains larger than 1 mm within vesicles of 1:1:1 bovine brain sphingomyelin/DOPC/chol with diameter >20 mm. They speculated that a z 1 at long observation times could be explained if domain merges triggered subsequent merges.…”

Section: Discussionmentioning

confidence: 99%

“…Deep physical questions about membrane dynamics can be posed if we allow the membrane to demix into two coexisting liquid phases such that domains enriched in one lipid type diffuse within a background membrane enriched in a different type (13)(14)(15)(16)(17)(18)(19). Differential partitioning of a dyelabeled lipid between the phases renders the domains straightforward to image on the surface of a GUV (Fig.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coarsening Dynamics of Domains in Lipid Membranes

Stanich

Honerkamp‐Smith

Putzel

et al. 2013

Biophysical Journal

104

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We investigate isothermal diffusion and growth of micron-scale liquid domains within membranes of free-floating giant unilamellar vesicles with diameters between 80 and 250 μm. Domains appear after a rapid temperature quench, when the membrane is cooled through a miscibility phase transition such that coexisting liquid phases form. In membranes quenched far from a miscibility critical point, circular domains nucleate and then progress within seconds to late stage coarsening in which domains grow via two mechanisms 1), collision and coalescence of liquid domains, and 2), Ostwald ripening. Both mechanisms are expected to yield the same growth exponent, α = 1/3, where domain radius grows as time(α). We measure α = 0.28 ± 0.05, in excellent agreement. In membranes close to a miscibility critical point, the two liquid phases in the membrane are bicontinuous. A quench near the critical composition results in rapid changes in morphology of elongated domains. In this case, we measure α = 0.50 ± 0.16, consistent with theory and simulation.

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Section: Introductionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Coarsening Dynamics of Domains in Lipid Membranes

Stanich

Honerkamp‐Smith

Putzel

et al. 2013

Biophysical Journal

104

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“…The kinetics of phase separation in multicomponent lipid membranes has been the subject of many experimental, theoretical and computational studies during the last decade [6-8,18-24]. Interleaflet coupling is typically assumed in the theoretical and computational studies.…”

Section: Introductionmentioning

confidence: 99%

“…Many in vitro experiments of multicomponent giant unilamellar lipid vesicles and supported bilayers have shown that these systems exhibit interesting lateral inhomogeneities in the form of liquid ordered domains, rich in cholesterol and saturated lipids, coexisting with liquid disordered domains rich in unsaturated lipids. [1][2][3][4][5][6][7][8][9] The understanding of domain formation in lipid membranes is particularly relevant to the lateral organization of plasma membranes of eukaryotic cells, which are inherently multi-component. Indeed, there currently exists a consensus that the plasma membrane of mammalian cells, in particular, exhibits nanoscale domains, known as lipid ras, which are rich in sphingomyelin and cholesterol.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of domain registration in multicomponent lipid bilayer membranes

et al. 2014

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The kinetics of registration of lipid domains in the apposing leaflets of symmetric bilayer membranes is investigated via systematic dissipative particle dynamics simulations. The decay of the distance between the centres of mass of the domains in the apposing leaflets is almost linear during early stages, and then becomes exponential during late times. The time scales of both linear and exponential decays are found to increase with decreasing the strength of interleaflet coupling. The ratio between the time scales of the exponential and linear regimes decreases with increasing the domain size, implying that the decay of the distance between the domains centres of mass is essentially linear for large domains. These numerical results are largely in agreement with the recent theoretical predictions of Han and Haataja [Soft Matter (2013) 9:2120-2124]. We also found that the domains become elongated during the registration process.

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Phase segregation in bio-inspired multi-component vesicles encompassing double tail phospholipid species

2014

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Our aim is to investigate the phase segregation and the structure of multi-component bio-inspired phospholipid vesicles via dissipative particle dynamics. The chemical distinction in the phospholipid species arises due to different head and tail group moieties, and molecular stiffness of the hydrocarbon tails. The individual amphiphilic phospholipid molecular species are represented by a hydrophilic head group and two hydrophobic tails. The distinct chemical nature of the moieties is modeled effectively via soft repulsive interaction parameters, and the molecular rigidity is tuned via suitable three-body potential constants. We demonstrate the formation of a stable hybrid vesicle through the self-assembly of the amphiphilic phospholipid molecules in the presence of a hydrophilic solvent. We investigate and characterize the phase segregation and the structure of the binary vesicles for different phospholipid mixtures. Our results demonstrate macroscopic phase separation for phospholipid mixtures composed of species with different hydrocarbon tail groups. We also investigate the relationship between the phase segregation and thermodynamic variables such as interfacial line tension and surface tension, and obtain correspondence between existing theory and experiments, and our simulation results. We report variations in the molecular chain stiffness to have negligible contributions to the phase segregation in the mixed bilayer, and to demonstrate shape transformations of the hybrid vesicle. Our results can be used to design novel bio-inspired hybrid vehicles for potential applications in biomedicine, sensing, imaging and sustainability.

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Domain growth dynamics in multicomponent vesicles composed of BSM/DOPC/cholesterol

Cited by 8 publications

References 35 publications

Coarsening Dynamics of Domains in Lipid Membranes

Coarsening Dynamics of Domains in Lipid Membranes

Kinetics of domain registration in multicomponent lipid bilayer membranes

Phase segregation in bio-inspired multi-component vesicles encompassing double tail phospholipid species

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