Dois novos sistemas de diagnose precoce da meleira do mamoeiro

Tavares, Eder T.; Tatagiba, Joseli S.; Ventura, José Aires; Souza, M.T.

doi:10.1590/s0100-41582004000500018

Cited by 8 publications

(9 citation statements)

References 2 publications

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Section: Introductionmentioning

confidence: 99%

“…Samples were obtained by injuring the fruit's peel and collecting the latex exuded from such fruits in 2mL microtubes which were then stored in an ultra freezer (-80ºC Genetic diversity studies of Papaya meleira virus in 2000. The presence of PMeV in the latex was assessed by extraction of viral dsRNA, treated with DNAse I and visualized in 1% agarose gels after electrophoresis, as described previously (Tavares et al, 2004).The samples that were proven positive for the presence of PMeV were selected, diluted in 0,1M ammonium citrate buffer, pH 6,0 and the viral dsRNA extracted using Brazol reagent (LGC Biotecnologia), according to the manufacturer's instructions. The final dsRNA pellet was dissolved in 20 µl of nuclease-free water, qualitatively analyzed by electrophoresis on 1% agarose gels and stored at -80°C.…”

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confidence: 99%

“…Samples were collected between the years 2008 and 2011, except for isolate PMeV-47, which was collected in 2000. The presence of PMeV in the latex was assessed by extraction of viral dsRNA, treated with DNAse I and visualized in 1% agarose gels after electrophoresis, as described previously (Tavares et al, 2004).…”

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Genetic diversity studies of Papaya meleira virus

Daltro¹,

Abreu²,

Aragão³

et al. 2014

Trop. plant pathol.

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Papaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya-producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Espírito Santo, Pernambuco, Ceará and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses. Key words: Carica papaya, Totiviridae, sticky disease, variability, viruses.Papaya (Carica papaya L.), is a fruit crop that originated and was domesticated in southern Mexico, and now is globally distributed and cultivated in many countries around the world. According to data from the Food and Agriculture Organization (FAO), the global production of papaya in 2010 was about 8 million tons, representing 10% of all tropical fruits produced worldwide (faostat.fao. org). The world's leading producers are India (42%) and Brazil (23.4%). Papaya production in Brazil was about 1,87 million tons on 35,000 hectares, with a value estimated at US$ 650 million (IBGE, 2011). The major papaya producing states are Bahia (928,000 tons), followed by Espírito Santo (560,000 tons), Ceará (112,000 tons) and Rio Grande do Norte (69,000 tons).The main factors that threaten papaya production are insect pests and diseases, especially those caused by viruses. The papaya sticky disease, caused by Papaya meleira virus (PMeV) was reported in the 1980's affecting papaya plants in the south of Bahia and north of Espírito Santo (Nakagawa et al., 1987;Rodrigues et al., 1989). Soon afterwards, the disease was detected in the states of Pernambuco, Ceara and Rio Grande do Norte (Barbosa et al., 1998a,b). Outside Brazil, Mexico is the only other country where the disease has been reported (Perez-Brito et al., 2012).The disease's typical symptoms are: excessive exudation of watery latex on the fruit surface, which oxidizes and gives the fruit a stained and sticky aspect; fruit malformation, uneven ripening and changes in the organolepctic properties, rendering it unmarketable.The PMeV genome composition and size are uncommon among plant viruses. PMeV has a double strand RNA (dsRNA) genome, approximately 12 Kb in length, packed into an isometric particle with ca. 50 nm diam (Kitajima et al., 1993; Maciel-Zambolin et al., 2003). The virus can be transmitted by latex injection into the stem apex, and previous work suggests that the virus could be transmitted by Bemisia ...

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Genetic diversity studies of Papaya meleira virus

Daltro¹,

Abreu²,

Aragão³

et al. 2014

Trop. plant pathol.

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“…As viroses do mamoeiro constituem, atualmente, o maior entrave à implantação de pólos produtores da cultura, devido à característica itinerante que lhe é imposta (KUNG et al, 2009;LIMA et al, 2001;RAMOS et al, 2008;SOUZA JÚNIOR et al, 2005). Dentre os principais vírus que infetam o mamoeiro, somente o vírus da mancha anelar do mamoeiro (Papaya ringspot virus, PRSV-P), família Potyviridae, gênero Potyvirus; o vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV), possível gênero Sobemovirus e o vírus da meleira (Papaya meleira virus, PSV), sem definição taxonômica foram registrados em pomares brasileiros (CAMARÇO et al, 1998;KITAJIMA et al, 1992;KUNG et al, 2009;LIMA et al, 2001;MARCIEL-ZAMBOLIM et al, 2003;RAMOS et al, 2008;TAVARES et al, 2004).…”

Section: Introductionunclassified

Sorologia e sobrevivência do vírus do amarelo letal do mamoeiro

et al. 2010

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Resumo -O vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV) é responsável por uma das principais doenças do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido através do solo, água, instrumento de corte e mãos contaminadas. A presente pesquisa teve como objetivo estudar as características biológicas, sorológicas e físicas de um isolado do vírus e avaliar sua sobrevivência em tecido seco infetado. O PLYV foi detectado por "Enzyme linked immunosorbent assay" (ELISA) indireto e isolado em mudas de mamoeiro, através da inoculação mecânica. A sobrevivência do PLYV em folhas e raízes secas de mamoeiro infetado foi avaliada por sorologia e inoculação em plantas sadias. A presença do vírus foi detectada em folhas e raízes de mamoeiro secas, indicando que o vírus pode permanecer ativo em restos de cultura por até 120 dias. A purificação do PLYV permitiu a obtenção de 309.5 mg de vírus/kg de folha e o anti-soro obtido mostrou-se altamente específico, com títulos de 1:128 em dupla difusão em Agar e 1:1.024.000 em Elisa indireto. Estudos das propriedades físicas do PLYV em mamoeiro revelaram um ponto de inativação térmica (PIT) em torno de 80 ºC, um ponto máximo de diluição (PMD) de ac. 10-6 e uma longevidade in vitro (LIV) acima de 50 dias. Palavras-chave -Carica papaya. PLYV. Papaya lethal yelloing virus.Abstract -The Papaya lethal yellowing virus (PLYV) is responsible for one of the most important disease of papaya (Carica papaya) in the Northeast of Brazil. The PLYV can be transmitted through the soil, irrigation water, agriculture tools and contaminated hands. The present research had the objective to characterize biologically, serologically and physically a PLYV isolate and evaluates how long it survives in infected dried tissues. The PLYV was identified by indirect enzyme linked immunoabsorbent assay (ELISA), isolated in young papaya plants by mechanical inoculations and maintained at green house conditions for virus purification. Approximately 309.5 mg of virus was purified per kg of infected papaya leaves and a polyclonal antiserum was obtained from an immunized rabbit. The antiserum obtained was shown to be highly specific to PLYV with a titer of 1:128 in double immune-diffusion and a titer of 1:1.024.000 in indirect ELISA. The virus was detected in dried roots and leaves maintained at room temperature up to 120 days, confirming its high stability within nonliving plant tissues, which could explain its dissemination by contaminated hands, tools, water and soil. The physical properties determined for the virus revealed a thermal inactivation point of 80 o C, longevity in vitro over 50 days and dilution end point ac. of 10 -6.

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“….docx -Google Docs https://docs.google.com/document/d/1zdnd3f8xaQ_8wGKrcUJ-isolated from the VLPs pellets using a procedure described for the extraction of PMeV-RNA in latex(70) . VLPs were incubated for 2 hours in the DNAse and RNAse cocktails (Invitrogen, Carlsbad, CA) with the addition of 14 µL of 20 mg/ml Proteinase K (Thermo Scientific, Waltham, MA) and the mixtures incubated at 37°C for 30 min.…”

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confidence: 99%

Network analysis of the papaya orchard virome from two agroecological regions of Chiapas, Mexico

Alcalá-Briseño

Casarrubias-Castillo²,

López-Ley³

et al. 2019

Preprint

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The study of complex ecological interactions - such as those among host, pathogen, and vector communities - can help to explain host ranges and the emergence of novel pathogens. The analysis of community structures using bipartite networks describe the associations between two trophic levels, for example plants and pollinators, or hosts and parasitoids. Bipartite networks represent interactions (links) occurring only between nodes in different levels - in our case, between viruses and hosts. We evaluated the viromes of papaya orchards (papaya, weeds, and insects) from intensive production of papaya in the Pacific Coastal Plain and the Central Depression of Chiapas, Mexico. Samples of papaya cultivar Maradol, which, like most cultivars, is susceptible to papaya ringspot virus (PRSV), were categorized by symptoms by local farmers (papaya ringspot symptoms, non-PRSV symptoms, or no symptoms). These analyses revealed the presence of 61 viruses, where only four species were shared among both physiographic regions. Nearly 52 complete viral genome sequences were recovered, of which 16 showed homology to known viruses, and 36 shared similarities with different genera including Potyvirus, Comovirus, and Tombusvirus (RNA viruses), and Begomovirus and Mastrevirus (DNA viruses). We analyzed the network of associations between viruses and host-location combinations, and described ecological properties of the network, such as asymmetry in interactions and nestedness compared to null models. Understanding the network structure informs management strategies, and advances understanding of interactions of hosts and viruses in the agroecological landscape.ImportanceVirus-virus interactions in plants can modify host symptoms. As a result, disease management strategies may be unsuccessful if they are based solely on visual assessment and diagnostic assays for known individual viruses. Papaya ringspot virus is an important limiting factor for papaya production, and likely has interactions with other viruses that are not yet known. Using high-throughput sequencing, we recovered known and novel RNA and DNA viruses from papaya orchards in Chiapas, Mexico, categorized by host and, in the case of papaya, symptom type: asymptomatic papaya, papaya with ringspot virus symptoms, papaya with non-ringspot symptoms, weeds, and insects. Using network analysis, we demonstrated virus associations within and among host types, and described the ecological community patterns. Recovery of viruses from weeds and asymptomatic papaya suggests the need for additional management attention. These analyses contribute to the understanding of the community structure of viruses in the agroecological landscape.

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Dois novos sistemas de diagnose precoce da meleira do mamoeiro

Cited by 8 publications

References 2 publications

Genetic diversity studies of Papaya meleira virus

Genetic diversity studies of Papaya meleira virus

Sorologia e sobrevivência do vírus do amarelo letal do mamoeiro

Network analysis of the papaya orchard virome from two agroecological regions of Chiapas, Mexico

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