DOG1, alpha-amylase and p63 expression in acinic cell carcinoma of salivary gland; immunohistochemical, clinical and radiological study

Abstract

Introduction: Identification of serous acinar differentiation is the basic step for the diagnosis of acinic cell carcinoma (ACC). α-amylase was described to be specific for normal acinar cell but its affinity to malignant counterpart is being contentious. Recently discovered marker anoctamin (DOG1) specific for diagnosis of GISTs has been reported for its efficacy to stain the malignant acinar cells and so, it is being of value in ACC diagnosis. On the other hand, negativity of acinar cells to basal cell marker as p63 will give more pronouncement to ACC diagnosis. Objectives: The study were designed to scrutinize the expression of recent marker DOG1 versus α-amylase in ACC diagnosis. Also to correlate between clinical, radiological, histopathological and immunohistochemical findings in ACC cases. Material and methods: Fourteen cases of ACC were obtained and stained immunohistochemically for DOG1, α-amylase and p63. Clinical data as well as radiological findings were obtained from the patients' file to form correlation with immunostaining results. Results: All ACCs (100%) revealed positivity toward DOG1 staining, 6 (44%) cases out of 14 showed α-amylase positivity, and 1 case (7%) showed focal positivity for p63. No significant correlation was found between clinical, radiological and immunostaining results. Significant correlation was obtained between results of DOG1 and α-amylase with p-value=0.034, also significant correlation was obtained between DOG1 and p63 with p-value=0.02. On the other hand, no significant correlation was obtained between results of both α-amylase and p63 with p-value=0.546. Conclusion: DOG1 has a higher efficacy in the diagnosis of ACC than α-amylase and must be used especially for cases with some conflicts as poor tissue sampling or suspicious cases with other carcinomas especially with carcinomas of clear cell features or having a vacuolated cytoplasm.

Acinic Cell Carcinoma of Nasal Cavity: A Rare Site for Salivary Gland Tumour

“…In a study of 14 cases of salivary gland acinic cell carcinoma by Ihab Shafek Atta et al, all ACCs (100%) revealed positivity with DOG1 staining and the authors concluded that DOG1 has a higher efficacy in the diagnosis of ACC than α-amylase. (10) The common pattern of DOG1 staining observed is that of intense apical membranous staining around lumina as in our case as well as complete membranous and variable cytoplasmic staining. (5) CONCLUSION Acinic cell carcinoma arising in nasal cavity is extremely uncommon.…”

Hepatocellular Carcinoma with Gastric Metastasis Mimicking a 4 cm Gastrointestinal Stromal Tumor After a 3-year Disease-free Interval

“…1A ). An additional immunohistochemical (IHC) study with glypican 3 3 and HepPar1 4 staining confirmed the diagnosis of metastatic HCC in the stomach ( Fig. 2G and H ).…”

Tyrosine phosphorylation of YAP‐1 in biliary epithelial cells mediates posthepatectomy liver regeneration and is affected by serotonin

“…Given extensive experimental evidence suggesting a central role for YAP‐1 in LR, we initially assessed intrahepatic YAP‐1 levels and dynamics in patients undergoing major liver resection. Baseline YAP‐1 expression was primarily found in ductular structures in the resting liver, as evaluated via co‐localization of YAP‐1 (marker reference 33 ) and CK19 (marker reference 34 ) upon immunofluorescence imaging (Figure 1). We then assessed tissue from the same patients obtained 2 h after induction of regeneration (study design illustrated in Figure 1A).…”