Dog leukocyte antigen‐88*034:01 presents nonamer peptides from canine distemper virus hemagglutinin, large polymerase, and matrix proteins

Abstract

Canine spontaneous cancers may offer greater fidelity than rodent models in advancing clinical immunotherapies. Boxers in particular are distinguished as study subjects by their popularity, and high incidence of human‐relevant cancers. Further, the MHC class I allele DLA‐88*034:01, with a known motif, dominates the breed, facilitating discovery of shared CTL responses against mutation‐origin neoepitopes by standard prediction methods. We experimentally confirmed the allomorph's binding motif by developing an MHC surface stabilization assay. The assay validated four DLA‐88*034:01‐presented peptides from canine distemper virus, ubiquitously administered in routine vaccines, for positive controls in future CTL studies. In turn, these viral peptides substantiated motif‐based prediction for DLA‐88*034:01. The study adds new tools for studying neoepitope‐specific CTL in Boxers to foster canine comparative oncology.

Genotyping of canine MHC gene DLA‐88 by next‐generation sequencing reveals high frequencies of new allele discovery and gene duplication

“…The most polymorphic of the class I DLA genes and the most informative of successful tissue/cell engraftment is DLA‐88 24 . Like class I HLA molecules in humans, the DLA‐88‐encoded protein binds and presents a wide variety of self and viral antigens 25–27 …”

“…24 Like class I HLA molecules in humans, the DLA-88-encoded protein binds and presents a wide variety of self and viral antigens. [25][26][27] Here we describe an NGS approach to DLA-88 genotyping. The approach was designed based on an amplicon-based system for HLA typing 10 that mirrors both the laboratory data acquisition steps and the computational analytical framework developed to determine genotypes using high-throughput technology.…”

“… 24 Like class I HLA molecules in humans, the DLA‐88‐encoded protein binds and presents a wide variety of self and viral antigens. 25 , 26 , 27 …”