Dog erythrocyte antigens (DEA) 1, 4, 7 and suspected naturally occurring anti-DEA 7 antibodies in Italian Corso dogs

Abstract

We sought to determine the prevalence of dog erythrocyte antigen (DEA) 1, 4 and 7 and naturally occurring anti-DEA7 antibodies in Italian Corso dogs. In addition, we correlated DEAs with different epidemiologic variables, compared the prevalence of DEAs against other canine populations and assessed the risk of sensitisation and transfusion reactions (TRs) following unmatched transfusion. Blood samples from 100 Corso dogs were evaluated for DEA 1, 4, 7 and naturally occurring anti-DEA 7 antibodies. Seventy-one percent of samples were DEA 1-negative, 100% tested DEA 4-positive, and 95% tested DEA 7-negative. Suspected anti-DEA7 antibodies were found in 32% dogs. The DEA 1 and 7-negative phenotypes were significantly more common than in most canine populations. When a previously tested Italian canine population was considered as blood donors for Corso dogs, the risk of DEA 1 sensitisation using DEA 1 untyped blood was 29%, and of acute haemolytic TRs after a second untyped DEA 1-incompatible transfusion was 8%. The potential for delayed TRs between DEA 7-negative Corso dogs with suspected naturally occurring anti-DEA 7 antibodies receiving untyped DEA 7-positive blood was 11%. Conversely, when Corso dogs were blood donors for the same population, the risk of DEA 1 sensitisation was 17% and the risk of an acute haemolytic TR after a second DEA 1-incompatible blood transfusion was 3%. Corso dogs can be suitable blood donors. Additional studies are needed to clarify whether the high prevalence of naturally occurring anti-DEA 7 antibodies in this breed could increase their risk of delayed TRs when they are blood recipients.

Survey of Blood Groups DEA 1, DEA 4, DEA 5, Dal, and Kai 1/Kai 2 in Different Canine Breeds From a Diagnostic Laboratory in Germany

“…Boxers and Cane Corso from various other European countries and South Africa were also mostly DEA 1-in prior surveys (details in Supplement Table 4). Thus, these breeds may be preferentially considered when recruiting blood donors to increase the DEA 1-donor pool, as suggested previously (25,42) In contrast, the Dalmatians and Pugs in our survey were all DEA 1+, except for one Dalmatian. Also, the Labrador Retriever and Shih Tzu breeds had mostly DEA 1+ dogs.…”

Imunohematologia Veterinária: Antígenos Eritrocitários Caninos

“…A prevalência de cães de DEA 1-positivos varia tanto geograficamente, que são apresentadas frequências entre 100% a <10%, tendo sido estimado em aproximadamente 40-60% sua prevalência internacional (CARLI et al, 2017;SPADA et al, 2017).…”

“…Após uma transfusão incompatível com DEA 1, o receptor de sangue torna-se sensibilizado e uma segunda transfusão incompatí-vel com DEA 1 pode resultar em uma reação transfusional aguda e potencialmente fatal (SPADA et al, 2017;TOCCI, 2010). Historicamente, são apresentados diferentes alelos no DEA 1, sendo DEA 1.1, DEA 1.2 e DEA 1.3.…”

“…É produzido em outro lugar do corpo em forma solúvel, secretado no plasma e adsorvido na membrana celular. A prevalência relatada de DEA 7 varia entre 6% e 82% em várias populações caninas (ESTEVES et al, 2011;SPADA et al, 2016bSPADA et al, , 2017.…”

“…Os sistemas sanguíneos caninos são divididos em DEA 1; (DEA 1.1; DEA 1,2; DEA 1,3); DEA 3; DEA 4; DEA 5; DEA 6; DEA 7 e DEA 8 e mais recentemente foram descritos o grupo DAL (encontrado em dálmatas) e DAI (EULER et al, 2016;LEE;GIGER;KIM, 2017;MEDINA VALENTIN;GAVAZZA;LUBAS, 2017). No entanto, pouco se sabe sobre muitos desses tipos de sangue e para alguns, como DEA 6 e 8, os soros de tipagem não existem mais (SPADA et al, 2017).…”