Dog cloning—no longer science fiction

Lee, Seok Hee; Oh, Hyun Ju; Kim, Min Jung; Setyawan, Erif Maha Nugraha; Ra, Kihae; Abdillah, Dimas Arya; Lee, Byeong Chun

doi:10.1111/rda.13358

Cited by 11 publications

(6 citation statements)

References 63 publications

(82 reference statements)

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“…SCNT can produce genetically identical animals, and the production performance of the cloned animal can be similar to that of the donor. Consequently, SCNT may be an effective method for the production of indigenous target animals for use in epigenetic research (Adachi et al, 2014;Keefer, 2015;Lee et al, 2018). However, somatic cell cloning has a low efficiency success rate, which may be affected by somatic cell type and oocyte activation status (Akagi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Methylation Analysis Reveals Epigenetic Variation in Cloned and Donor Pigs

Wang

Feng

et al. 2020

Front. Genet.

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Somatic cloning has had a significant impact on the life sciences and is important in a variety of processes, including medical research and animal production. However, the application of somatic cloning has been limited due to its low success rate. Therefore, potential epigenetic variations between cloned and donor animals are still unclear. DNA methylation, one of the factors which is responsible for phenotypic differences in animals, is a commonly researched topic in epigenetic studies of mammals. To investigate the epigenetic variations between cloned and donor animals, we selected blood and ear fibroblasts of a donor pig and a cloned pig to perform whole-genome bisulfite sequencing (WGBS). A total of 215 and 707 differential methylation genes (DMGs) were identified in blood and ear fibroblasts, respectively. Functional annotation revealed that DMGs are enriched in many pathways, including T/B or natural killer (NK) cell differentiation, oocyte maturation, embryonic development, and reproductive hormone secretion. Moreover, 22 DMGs in the blood and 75 in the ear were associated with immune responses (e.g., CD244, CDK6, CD5, CD2, CD83, and CDC7). We also found that 18 DMGs in blood and 53 in ear fibroblasts were involved in reproduction. Understanding the expression patterns of DMGs, especially in relation to immune responses and reproduction, will reveal insights that will aid the advancement of future somatic cloning techniques in swine.

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Section: Introductionmentioning

confidence: 99%

Whole-Genome Methylation Analysis Reveals Epigenetic Variation in Cloned and Donor Pigs

Wang

Feng

et al. 2020

Front. Genet.

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“…In stark contrast with the human sector, animal genetic research is able to dabble in what most would believe to be science fiction. Somatic Cell Nuclear Transfer (SCNT), also referred to as “cloning,” has been applied to the companion animal industry [ 43 ] in which pet guardians are able to “genome bank” their cherished pet’s genetic material and eventually use this to create a clone (e.g., Viagen [ 44 ]). At the beginning of 2022, an article was released by Input Magazine detailing certain social media influencers had either already done so or put plans in place to clone their pet who had also become a social media celebrity [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

A Study Evaluating Consumer Motivations, Perceptions, and Responses to Direct-to-Consumer Canine Genetic Test Results

Bennett

Gray

2022

Animals

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Direct-to-consumer genetic services allow companion animal guardians to purchase a DNA test and receive detailed results about their pet’s ancestry, health, and traits results. In collaboration with Wisdom Panel, we present novel findings about consumer motivations, perceptions, and responses to their use of canine genomic services. Wisdom Panel customers were invited to complete an online survey anonymously in which they were asked about their reasons for using a genetic test for their dog, how they perceived the test’s results, and how they responded to the results they received. Participant data revealed most utilized a test that provided more ancestry/breed results (75.9%) as compared to health-related results. The majority of participants perceived the breed test results as accurate (52.0% strongly agree, 27.6% somewhat agree) and the genetic services provided as having great value (49.6% strongly agree, 32.7% somewhat agreed). In responding to their dog’s results, participants indicated they shared the information with family (88.1%) and friends (84.2%). Collectively, our study indicates consumers are more focused on their dog’s ancestry than other test results. Using these findings and previous literature on human direct-to-consumer genetic testing, human–animal dyads, and identity construction, we consider the possibility of “breed options theory” and future areas of research.

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“…The cloning of dogs was first reported in 2005 by Lee et al (2005) and is reviewed in the current issue of this journal entitled "Dog cloning -no longer science fiction" by Lee et al (2018). Although the pregnancy rate and number of live born puppies is below that occurring after natural service and AI, a 50% full term pregnancy rate and 5%-6% survival rate to term of cloned embryos was reported by Jeong et al (2016).…”

Section: Cloning (Somatic Cell Nuclear Transfer)mentioning

confidence: 99%

Ethics in animal breeding

Farstad

2018

Reprod Domestic Animals

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Contents Ethical breeding involves the use of healthy animals true to their species in behaviour and physical appearance, and when applicable, showing a sustainable performance. The concerns for the species/breed are essential parts of the breeding goals, including preservation of genetic resources within the species/breed, and the health and welfare of the individual animal. Ethical and welfare considerations were often not prioritized in developing new breeds of production or companion animals. As a result, animal breeding practices are increasingly becoming part of the debate on animal welfare. In companion animals, breeding for curiosity or “cuteness” may be a goal in itself, although dogs are also bred for utility. In production animals, breeding focus is on performance, i.e., quantitative entities and financial income, rather than physical appearance. For instance, dairy cows are bred to be larger and to have higher milk yields, sows and ewes to produce more offspring, and horses are designed for riding, racing, and companionship. Overbreeding in relation to current demand of horses, cats, and dogs raises welfare issues due to abandonment or killing of horses and millions of cats and dogs every year. There is variable regulation of health requirements for breeding animals in different countries of the world. In many countries, consumers are becoming increasingly aware of animal welfare issues such as negative effects of certain production traits in farm animals, leading to decreased demand for their meat at a time where increased food production is becoming crucial. Amidst these dilemmas are the veterinarians. This paper deals with issues connected to traditional breeding as well as some of the breeding technologies, and includes food safety, ethics, and animal welfare.

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Dog cloning—no longer science fiction

Cited by 11 publications

References 63 publications

Whole-Genome Methylation Analysis Reveals Epigenetic Variation in Cloned and Donor Pigs

Whole-Genome Methylation Analysis Reveals Epigenetic Variation in Cloned and Donor Pigs

A Study Evaluating Consumer Motivations, Perceptions, and Responses to Direct-to-Consumer Canine Genetic Test Results

Ethics in animal breeding

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