Dog and Cat Allergies and Allergen Avoidance Measures in Korean Adult Pet Owners Who Participated in a Pet Exhibition

Yang, Min; Lee, Sang Pyo; Kwon, Young Jae

doi:10.4168/aair.2018.10.2.155

Cited by 19 publications

(20 citation statements)

References 40 publications

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“…According to a recent study, the most frequent allergic disease entities of cat-allergic patients are rhinitis (80.0%), followed by conjunctivitis (73.3%) and cutaneous symptoms (33.3%). 36 Interestingly, in all tested patients, there was a gender difference between Cat-NPC2-associated allergic disease entities. In fact, striking sex-specific biases have been observed in IgE-mediated allergic diseases such as respiratory allergies, 37 food allergy 38 and anaphylaxis.…”

Section: Discussionmentioning

confidence: 85%

Cat NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7

Zhu

et al. 2021

Allergy Asthma Immunol Res

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Purpose Pet-derived allergens are the common indoor inhalant allergens. Among them, cat and dog allergens constitute more than 80% of animal allergic patients, which greatly affect the quality-of-life of patients and increase the burden of social health care. The aim of this study was to identify Cat-Niemann pick type C2 (NPC2) protein, a homologue of Can f 7, as a new allergen. Methods Cat-NPC2 complementary DNA (cDNA) was cloned and optimized for amplification and expression in Escherichia coli . Then, recombinant Cat-NPC2 (rCat-NPC2) was purified by Ni 2+ affinity chromatography. The allergenicity was assessed by enzyme-linked immunosorbent assay (ELISA), western blot and basophil activation test (BAT). Based on the sequence similarity, the cross-reactivity between Cat-NPC2 and Can f 7 was investigated by inhibition ELISA. Circular dichroism spectroscopy and homology modeling were used to characterize the structure of Cat-NPC2. Results The cDNA sequence of Cat-NPC2 was cloned with a 450-bp open reading frame coding for 149 amino acids (GenBank MN_737596). The condon-optimized NPC2 gene was subcloned and expressed in E. coli with a molecular weight of 18.9 kDa. The native Cat-NPC2 was detected in cat dander extracts. The allergenicity determined by ELISA, western blot and BAT suggested at least 14.5% cat-allergic patients displayed high specific immunoglobulin E (IgE) recognition of Cat-NPC2. The predicted structure of Cat-NPC2 was found to consist of 7 β-strands arranged in 2 β-sheets. An ELISA based assay showed that rCat-NPC2 bound to cholesterol in a dose dependent manner. Based on the structure and sequence similarities, IgE cross-reactivity was demonstrated between Cat-NPC2 and Can f 7/Der f 2. Conclusions In the study, a novel cat allergen, belonging to the NPC2 protein family, was identified and characterized at both molecular and immunological levels. The study will offer a deeper understanding of cat allergens and improve a component-resolved diagnosis in pet allergy.

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Section: Discussionmentioning

confidence: 85%

Cat NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7

Zhu

et al. 2021

Allergy Asthma Immunol Res

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“…The most frequent symptoms of cat owners suffering from allergy when exposed to contact with their pets were rhinitis (sneezing, rhinorrhea, nasal obstruction and itching, postnasal drip) (80.0%), followed by conjunctivitis (73.3%), cutaneous symptoms (skin itching, skin rash, angioedema, urticaria) (33.3%), and lower respiratory symptoms (sputum, dyspnea, chest discomfort and pain, wheezing) (13.3%). Dog owners with allergy to their pets suffered from cutaneous symptoms more frequently than cat owners with cat allergy (53.3% vs. 33.3%, P < 0.01), especially in terms of urticaria (35.9% vs. 15.6%, P < 0.01) as well as from lower respiratory symptoms (33.3% vs 13.3%, P < 0.05), especially cough (23.3% vs 6.7%, P < 0.05) [19] .…”

Section: Discussionmentioning

confidence: 98%

Sensitization to cat and dog components and prediction of symptoms in cat-sensitized children.

Smejda

Podlecka

Brzozowska

2020

Preprint

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Background: Sensitization to cat and/or dog allergens during childhood represents a risk factor for the development of allergic diseases later in life.Material and Methods: The study investigated the association of patterns of sensitization to cat and dog allergen components with clinical symptoms of allergy to these furry animals among cat-sensitized children. It included 50 children aged 5-17 years who showed sensitization to cat in the skin prick test. They were considered to have cat allergy if they suffered from one or more allergy symptoms when being exposed to contact with a cat. The children were evaluated for the presence of bronchial asthma, atopic dermatitis and allergic rhinitis. Their mothers completed a questionnaire on pet exposure at home. Levels of serum IgE cat epitopes Fel d (1, 2, 4), as well as dog components Can f (1, 2, 3, 5) were measured in all the studied children.Results: Respiratory symptoms following exposure to the cat allergen were most common in children with Fel d 2 epitope (p = 0.041). After contact with a dog, respiratory symptoms were most common in children with Can f 1 epitope (p = 0.042), eczema in children with sensitization to both Can f 1 (p = 0.009) and Can f 2 (p = 0.002), whereas eye symptoms occurred mostly in children with Can f 3 (p = 0.039).Conclusions: Molecular diagnosis in patients with pet allergy may help clinicians to predict clinical symptoms and their severity.

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“…This is similar to the findings of some previous studies. They have reported that Can f 1 is detected in about one-third of houses that do not own a dog [ 5 8 16 ]. Members of such houses were found to frequently visit a relative who owns a dog, and our findings supported previous results.…”

Section: Discussionmentioning

confidence: 99%

“…Pet ownership in Seoul has increased rapidly, with 20.4% of the population having pets in 2016, and the prevalence of dog ownership among pet owners (88.5%) being remarkably higher than that of cat ownership (26.6%) [ 4 5 ]. Sensitization rates to dogs have not been reported among Korean children, but among adults, they are known to be 7.6% [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Sensitization rates to dogs have not been reported among Korean children, but among adults, they are known to be 7.6% [ 6 ]. Even without having a pet in their own homes, children may be at risk of dog or cat allergies because animal allergens can be distributed in public places or at the homes of relatives [ 5 7 ]. Previous studies have indicated that sensitization to pets from indirect exposure and a moderate dose of allergen concentration was more frequently observed in allergic patients compared to direct exposure and a high concentration of allergen [ 7 8 ].…”

Section: Introductionmentioning

confidence: 99%

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IgE recognition profile of aeroallergen components in young children sensitized to dogs

Lee

Jeong

Jeon

et al. 2020

Asia Pacific Allergy

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Background Pet ownership is increasing rapidly and as growing numbers of dogs in household, clinicians are facing more allergic patients and so as in young children. Objective This study aims to profile the IgE recognition patterns to aeroallergen components in young children sensitized to dogs. Methods Through retrospective chart reviews, we evaluated the clinical, environmental, and laboratory findings of patients sensitized to dogs in early life. We further evaluated specific IgE to dog component allergens (Can f 1, Can f 2, and Can f 3) and other aeroallergens using a microarray. Results The median age of 28 patients sensitized to dogs (dog-specific IgE ≥ 0.35 kU/L; 0.38–101 kU/L) was 61 months and underlying diseases included doctor diagnosed atopic dermatitis (n = 17), asthma (n = 7), and allergic rhinitis (n = 5). Twenty patients (71.4%) had experienced self-reported dog allergy and 70.0% of them were symptomatic after exposed to dogs from others. Component-resolved diagnosis was performed on 18 patients. Can f 1 positivity was the most common (77.8%) but had no value in symptom prediction. The most common cosensitized aeroallergen was house dust mites (44.5%). The symptomatic group tended to be poly-sensitized to Can f 1, Can f 2, and Can f 3. Conclusion Can f 1 was dominantly detected and poly-sensitized to Can f 2 and/or Can f 3 simultaneously tend to develop hypersensitivity to dogs in young children. Most of them were exposed to dogs not living with.

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Dog and Cat Allergies and Allergen Avoidance Measures in Korean Adult Pet Owners Who Participated in a Pet Exhibition

Cited by 19 publications

References 40 publications

Cat NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7

Cat NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7

Sensitization to cat and dog components and prediction of symptoms in cat-sensitized children.

IgE recognition profile of aeroallergen components in young children sensitized to dogs

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