DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

Santopolo, Silvia; Boccaccini, Alessandra; Lorrai, Riccardo; Ruta, Veronica; Capauto, Davide; Minutello, Emanuele; Serino, Giovanna; Costantino, Paolo; Vittorioso, Paola

doi:10.1186/s12870-015-0453-1

Cited by 47 publications

(33 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly DAG2, the DOF protein which plays an antagonistic role to DAG1 [72], has been shown to negatively control expression of RGA, but not of GAI. Indeed, RGA transcript level is significantly increased in dag2 mutant seeds exposed to Red light, consistently with the function of DAG2 as a positive regulator of seed germination in the PIF1-DAG1 molecular pathway [79].…”

Section: Interaction Dof-della Represses Seed Germinationsupporting

confidence: 67%

The DOF Transcription Factors in Seed and Seedling Development

Ruta

Longo

Lepri

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

The DOF (DNA binding with one finger) family of plant-specific transcription factors (TF) was first identified in maize in 1995. Since then, DOF proteins have been shown to be present in the whole plant kingdom, including the unicellular alga Chlamydomonas reinhardtii. The DOF TF family is characterised by a highly conserved DNA binding domain (DOF domain), consisting of a CX 2 C-X 21 -CX 2 C motif, which is able to form a zinc finger structure. Early in the study of DOF proteins, their relevance for seed biology became clear. Indeed, the PROLAMIN BINDING FACTOR (PBF), one of the first DOF proteins characterised, controls the endosperm-specific expression of the zein genes in maize. Subsequently, several DOF proteins from both monocots and dicots have been shown to be primarily involved in seed development, dormancy and germination, as well as in seedling development and other light-mediated processes. In the last two decades, the molecular network underlying these processes have been outlined, and the main molecular players and their interactions have been identified. In this review, we will focus on the DOF TFs involved in these molecular networks, and on their interaction with other proteins.

show abstract

Section: Interaction Dof-della Represses Seed Germinationsupporting

confidence: 67%

The DOF Transcription Factors in Seed and Seedling Development

Ruta

Longo

Lepri

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interestingly DAG2, the DOF protein which plays an antagonistic role to DAG1 [65], has been shown to negatively control expression of RGA, but not of GAI. Indeed, RGA transcript level is significantly increased in dag2 mutant seeds exposed to Red light, consistently with the function of DAG2 as a positive regulator of seed germination in the PIF1-DAG1 molecular pathway [72].…”

Section: Dof Proteins In Seed Maturationsupporting

confidence: 67%

The DOF Transcription Factors in Seed and Seedling Development

Ruta¹,

Longo²,

Lepri³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

The DOF (DNA binding with one finger) family of plant-specific transcription factors (TF) was first identified in maize in 1995. Since then, DOF proteins have been shown to be present in the whole plant kingdom including the unicellular alga Chlamydomonas reinhardtii. The DOF TF family is characterised by a highly conserved DNA binding domain (DOF domain), consisting of a CX2C-X21-CX2C motif which is able to form a zinc finger structure. Early in the study of DOF proteins it became clear their relevance for seed biology. Indeed, the Prolamine Binding Factor (PBF), one of the first DOF proteins characterised, controls the endosperm-specific expression of the zein genes in maize. Subsequently, several DOF proteins from both monocots and dicots have been shown to be primarily involved in seed development, dormancy and germination, as well as in seedling development and other light-mediated processes. In the last two decades the molecular network underlying these processes have been outlined, and the main molecular players and their interactions have been identified. In this review, we will focus on the DOF TFs involved in these molecular networs, and on their interaction with other proteins.

show abstract

“…As the seeds were transferred to light after two days of stratification, TFs from the DOF (DNA-binding with one finger) family were inferred to play prominent roles in the upregulation of several transcription modules, which is consistent with their known roles in growth and development [ 52 ]. One example is DAG2, a positive regulator of light-mediated germination [ 53 ], which is upregulated and annotates two branches that are strongly upregulated after exposure to light (Fig. 4 ; Additional file 2 : Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Extensive transcriptomic and epigenomic remodelling occurs during Arabidopsis thaliana germination

et al. 2017

View full text Add to dashboard Cite

BackgroundSeed germination involves progression from complete metabolic dormancy to a highly active, growing seedling. Many factors regulate germination and these interact extensively, forming a complex network of inputs that control the seed-to-seedling transition. Our understanding of the direct regulation of gene expression and the dynamic changes in the epigenome and small RNAs during germination is limited. The interactions between genome, transcriptome and epigenome must be revealed in order to identify the regulatory mechanisms that control seed germination.ResultsWe present an integrated analysis of high-resolution RNA sequencing, small RNA sequencing and MethylC sequencing over ten developmental time points in Arabidopsis thaliana seeds, finding extensive transcriptomic and epigenomic transformations associated with seed germination. We identify previously unannotated loci from which messenger RNAs are expressed transiently during germination and find widespread alternative splicing and divergent isoform abundance of genes involved in RNA processing and splicing. We generate the first dynamic transcription factor network model of germination, identifying known and novel regulatory factors. Expression of both microRNA and short interfering RNA loci changes significantly during germination, particularly between the seed and the post-germinative seedling. These are associated with changes in gene expression and large-scale demethylation observed towards the end of germination, as the epigenome transitions from an embryo-like to a vegetative seedling state.ConclusionsThis study reveals the complex dynamics and interactions of the transcriptome and epigenome during seed germination, including the extensive remodelling of the seed DNA methylome from an embryo-like to vegetative-like state during the seed-to-seedling transition. Data are available for exploration in a user-friendly browser at https://jbrowse.latrobe.edu.au/germination_epigenome.Electronic supplementary materialThe online version of this article (doi:10.1186/s13059-017-1302-3) contains supplementary material, which is available to authorized users.

show abstract

DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

Cited by 47 publications

References 37 publications

The DOF Transcription Factors in Seed and Seedling Development

The DOF Transcription Factors in Seed and Seedling Development

The DOF Transcription Factors in Seed and Seedling Development

Extensive transcriptomic and epigenomic remodelling occurs during Arabidopsis thaliana germination

Contact Info

Product

Resources

About