Does trimetazidine act as antioxidant?

Abstract

Trimetazidine is 2-fold inferior to probucol in antioxidant activity measured using the model of copper-induced free radical oxidation of human plasma lipoproteins. EPR-spectroscopy shows that trimetazidine forms no free-radical intermediates in the presence of generated lipid alkoxyl or hydroxyl radicals, while probucol under these conditions forms phenoxyl radicals. Trimetazidine does not interact with superoxide radicals generated in the xanthinexanthine oxidase system, since it does not inhibit reduction of tetrazolium nitroblue. However, indirect effect of trimetazidine on free radical oxidation cannot be excluded in vivo.

Relationship between free-radical lipid oxidation and efficiency of coronary angioplasty in coronary patients

“…After dialysis, LDL were diluted to a concentration of 50 μg protein/ml and incubated in a medium with 0.154 M NaCl and 50 mM phosphate buffer (pH 7.4) at 37 o C with 3×10 -5 M CuSO 4 and lipoperoxide accumulation was measured at λ=233 on a Hitachi 220A spectrophotometer after certain intervals [4]. The duration of the lag phase of LDL oxidation was evaluated by kinetic curves, hydroperoxide content in LDL was evaluated by the reaction with Fe 2+ xylene orange before and after their reduction with triphenylphosphine on a Hitachi-557 spectrophotometer at λ=560 nm [9].…”

Antioxidant probucol as an effective scavenger of lipid radicals in low density lipoproteinsin vivo andin vitro

“…Thereafter, the plasma was dialized for 16 h at 4~ Protein content was determined according to Lowry and LDL concentration was adjusted to 50 ~tg protein/ ml with 50 mM phosphate buffer (pH 7.4) containing 0.154 M NaC1. LDL oxidation was induced with 3x10 5 M CuSO 4 and accumulation of lipohydroperoxides was measured on a Hitachi 220A spectrophotometer at 233 nm at fixed time intervals [5]. LDL from healthy donors without hyperlipidemia were oxidized in the presence of PB (Sigma) added to the incubation medium as an ethanol solution (2% final ethanol concentration).…”