“…It can be summarized that toothbrushing, aimed at removing dental plaque, is an essential element of periodontal treatment and control of pocket infections. It could be shown that beyond mechanical cleaning, stimulation of tissues by toothbrushing provided several benefits, including an increase in keratinization of the oral epithelium, capillary gingival circulation, proliferative activity of the junction basal cells as well as the repair of small breaks in the junctional epithelium and prevention of direct access of periodontal pathogens into the gingival tissue (56,61,78,121,122). However, modern concepts for the prevention and therapy of biofilm-associated infections, such as periodontitis (and also gingivitis, caries and endodontic infections), should not solely be based on mechanical removal, but also on the use of adjuvant substances with antibacterial properties (100,108).…”