Does the vesicular stomatitis virus really have a selective oncolytic effect in human cancer?

Yasmeen, Amber; Zhang, Li; Moustafa, Ala‐Eddin Al

doi:10.1002/ijc.24922

Cited by 6 publications

(7 citation statements)

References 7 publications

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“…Cell Lines Used. Human oral epithelial cells (HOEC) 39 and human mammary epithelial cells (HMEC) 40 used in this study were immortalized by transfection with retroviral vectors containing E6/E7 oncoproteins of high-risk human papillomavirus (HPV) type 16. The HaCat keratinocyte cell line (spontaneously transformed immortal cell line), obtained from adult human skin, was used at passage 48 in this study.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Augustine

Hasan

Patan

et al. 2019

ACS Biomater. Sci. Eng.

140

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Insufficient cell proliferation, cell migration, and angiogenesis are among the major causes for nonhealing of chronic diabetic wounds. Incorporation of cerium oxide nanoparticles (nCeO2) in wound dressings can be a promising approach to promote angiogenesis and healing of diabetic wounds. In this paper, we report the development of a novel nCeO2 containing electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membrane for diabetic wound healing applications. In vitro cell adhesion studies, chicken embryo angiogenesis assay, and in vivo diabetic wound healing studies were performed to assess the cell proliferation, angiogenesis, and wound healing potential of the developed membranes. The experimental results showed that nCeO2 containing PHBV membranes can promote cell proliferation and cell adhesion when used as wound dressings. For less than 1% w/w of nCeO2 content, human mammary epithelial cells (HMEC) were adhered parallel to the individual fibers of PHBV. For higher than 1% w/w of nCeO2 content, cells started to flatten and spread over the fibers. In ovo angiogenic assay showed the ability of nCeO2 incorporated PHBV membranes to enhance blood vessel formation. In vivo wound healing study in diabetic rats confirmed the wound healing potential of nCeO2 incorporated PHBV membranes. The study suggests that nCeO2 incorporated PHBV membranes have strong potential to be used as wound dressings to enhance cell proliferation and vascularization and promote the healing of diabetic wounds.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Augustine

Hasan

Patan

et al. 2019

ACS Biomater. Sci. Eng.

140

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show abstract

“…VSV has been shown to cause viral encephalitis in animal models. In addition, some scientists are skeptical regarding VSV safety and oncoselectivity; therefore, clinical safety following intravenous administration of VSV is given the highest priority, especially in individuals with immune systems compromised by disease or chemotherapy [6] . Despite the fact that neurotoxicity has been observed following intravascular VSV administration, little is known about the interaction of VSV with human peripheral blood leukocytes (PBLs) [ 6 , 7 and references therein].…”

Section: Immune Consequences Of In Vitro Infectionmentioning

confidence: 99%

Immune Consequences of in vitro Infection of Human Peripheral Blood Leukocytes with Vesicular Stomatitis Virus

Tomczyk

Wróbel²,

Chaber³

et al. 2018

J Innate Immun

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Background: Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. Our study aimed to assess the overall immunological consequences of ex vivo infection of PBLs with VSV. Methods: Phenotypic analysis of lymphocyte subsets and apoptosis were evaluated with flow cytometry. Caspase 3/7 activity was detected by luminescence assay. Virus release was evaluated in a murine cell line (L929). Gene expression and cytokine/chemokine secretion were assessed by real-time PCR and multiplex assay, respectively. Results: Ex vivo infection of PBLs with VSV elicited upregulated expression of RIG-I, MDA-5, tetherin, IFITM3, and MxA. VSV infection triggered rapid differentiation of blood monocytes into immature dendritic cells as well as their apoptosis, which depended on caspase 3/7 activation. Monocyte differentiation required infectious VSV, but loss of CD14+ cells was also associated with the presence of a cytokine/chemokine milieu produced in response to VSV infection. Conclusions: Systemic delivery is a major goal in the field of oncolytic viruses. Our results shed further light on immune mechanisms in response to VSV infection and the underlying VSV-PBL interactions bringing hope for improved cancer immunotherapies, particularly those based on intravenous delivery of oncolytic VSV.

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“…Cells were grown in complete cell culture medium Gibco® RPMI-1640 (Life Technologies, Burlington, ON, Canada) supplemented with 5% fetal bovine serum (FBS; Invitrogen, Life Technologies) and 1% PenStrep antibiotic (Invitrogen, Life Technologies). Human normal mammary epithelial cells immortalized by the E6/E7 gene of HPV type 16 (HNME-E6/E7) [41] were used as a control. These cells were maintained in Gibco® Keratinocyte-SFM (1X) medium (Thermo Fisher Scientific, Mississauga, ON, Canada) supplemented with 1% PenStrep antibiotic (Thermo Fisher Scientific, Mississauga, ON, Canada).…”

Section: Cell Culturementioning

confidence: 99%

Targeting HER2-positive breast cancer cells by a combination of dasatinib and BMS-202: Insight into the molecular pathways

Kheraldine,

Gupta,

Cyprian

et al. 2024

Cancer Cell Int

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Background Recent investigations have reported the benefits of using a tyrosine kinase inhibitor, dasatinib (DA), as well as programmed death-ligand 1 (PD-L1) inhibitors in the management of several solid tumors, including breast cancer. Nevertheless, the outcome of the combination of these inhibitors on HER2-positive breast cancer is not explored yet. Methods Herein, we investigated the impact of DA and PD-L1 inhibitor (BMS-202) combination on HER2-positive breast cancer cell lines, SKBR3 and ZR75. Results Our data reveal that the combination significantly inhibits cell viability of both cancer cell lines as compared to monotreatment. Moreover, the combination inhibits epithelial-mesenchymal transition (EMT) progression and reduces cancer cell invasion by restoring E-cadherin and β-catenin expressions and loss of vimentin, major biomarkers of EMT. Additionally, the combination reduces the colony formation of both cell lines in comparison with their matched control. Also, the combination considerably inhibits the angiogenesis of the chorioallantoic membrane model compared with monotreatment. Molecular pathway analysis of treated cells shows that this combination blocks HER2, AKT, β-catenin, and JNK1/2/3 activities. Conclusion Our findings implicate that a combination of DA and BMS-202 could have a significant impact on the management of HER2-positive breast cancer.

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Does the vesicular stomatitis virus really have a selective oncolytic effect in human cancer?

Cited by 6 publications

References 7 publications

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Immune Consequences of in vitro Infection of Human Peripheral Blood Leukocytes with Vesicular Stomatitis Virus

Targeting HER2-positive breast cancer cells by a combination of dasatinib and BMS-202: Insight into the molecular pathways

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