Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis

Murugesu, Sughashini; Saso, Srdjan; Jones, Benjamin P; Bracewell‐Milnes, Timothy; Athanasiou, Thanos; Mania, Anastasia; Serhal, Paul; Ben-Nagi, Jara

doi:10.1016/j.fertnstert.2017.06.029

Cited by 64 publications

(41 citation statements)

References 37 publications

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“…Ca 2+ ionophores like ionomycin, calcimycin are currently used to overcome unexplained fertilization failure in couples, who repeatedly fail ICSI cycles (Fawzy et al, 2018;Norozi-Hafshejani et al, 2018). Recently, a meta-analysis indicated that use of Ca 2+ ionophores significantly improved fertilization and implantation rates in ICSI (Murugesu et al, 2017). However, Ca 2+ ionophores induce a single Ca 2+ transient, unlike the endogenous specific physiological pattern of Ca 2+ oscillations observed during normal fertilization (Rinaudo et al, 1997).…”

Section: Clinical Applications Of Plcζ and Future Directionsmentioning

confidence: 99%

Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update

Saleh

Kashir

Thanassoulas

et al. 2020

Front. Cell Dev. Biol.

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Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus responsible for generating calcium (Ca 2+) oscillations that induce egg activation and early embryonic development during mammalian fertilization. In the mammalian testis, PLCζ expression is detected at spermiogenesis following elongated spermatid differentiation. Sperm-delivered PLCζ induces Ca 2+ release via the inositol 1,4,5trisphosphate (InsP 3) signaling pathway. PLCζ is the smallest known mammalian PLC isoform identified to date, with the simplest domain organization. However, the distinctive biochemical properties of PLCζ compared with other PLC isoforms contribute to its unique potency in stimulating cytosolic Ca 2+ oscillations within mammalian eggs. Moreover, studies describing PLCζ "knockout" mouse phenotypes confirm the supreme importance of PLCζ at egg activation and monospermic fertilization in mice. Importantly, a number of clinical reports have highlighted the crucial importance of PLCζ in human fertilization by associating PLCζ deficiencies with certain forms of male factor infertility. Herein, we give an update on recent advances that have refined our understanding of how sperm PLCζ triggers Ca 2+ oscillations and egg activation in mammals, while also discussing the nature of a potential "alternative" sperm factor. We summarise PLCζ localization in mammalian sperm, and the direct links observed between defective PLCζ protein in sperm and documented cases of male infertility. Finally, we postulate how this sperm protein can be used as a potential diagnostic marker, and also as a powerful therapeutic agent for treatment of certain types of male infertility due to egg activation failure or even in more general cases of male subfertility.

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Section: Clinical Applications Of Plcζ and Future Directionsmentioning

confidence: 99%

Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update

Saleh

Kashir

Thanassoulas

et al. 2020

Front. Cell Dev. Biol.

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“…Calcium ionophore treatment results in a statistically significant improvement of fertilization, blastulation, and implantation rates, as well as of pregnancy and live-birth rates. This demonstrates a strong positive effect of calcium ionophore use [12].…”

Section: Artificial Activation Of Oocytesmentioning

confidence: 74%

Current Topics in Assisted Reproduction in the Czech Republic

Žáková

Crha

Jelínková³

et al. 2018

Medical Journal of Cell Biology

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Assisted reproduction is a very dynamic part of reproductive gynaecology with fast changes in therapeutic approaches resulting from intensive research supported by private or public companies. This progress brings also new questions regarding ethical, legal or therapeutic issues. At the 26th Czech-Slovak symposium of assisted reproduction in Brno, several interesting topics of assisted reproduction were presented. During two days, more than 350 specialists discussed current topics related to gynaecology, embryology, andrology, genetics and sexology. From this fruitful discussion we chose the following topics that we consider to be the most important: (1) “Soft” versus “standard” stimulation protocols for IVF (2) Use of exogenous and endogenous progestins in IVF stimulation protocols (3) Cryopreservation and transplantation of ovarian tissue - state of the art and situation in the Czech Republic (4) Selection of spermatozoa with fragmented DNA: practical recommendations (5) Artificial activation of oocytes (8) Access of single and lesbian women to assisted reproduction technologies and possibility of their reproduction in the Czech Republic. Panel conclusions were presented at the end of the conference, which had great attendance, invoked lively commentaries and produced some definitive consensus. Certain issues remained inconclusive and these matters will be the subject of further discussion in future. This is a summary of the most important theses from this field in the Czech Republic in 2017.

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“…It has been found that embryo implantation and live birth rates were very low in patients with AOA after ICSI (1,11,13,14) and many pregnancies were lost during the first trimester (1) although it was not known if this was resulting from poor sperm quality and/or poor oocyte activation. Aneuploidy is high in human embryos produced by IVF, even in young women (29), suggesting that low implantation rates and early pregnancy lose reported in the previous studies may be partially due to embryonic aneuploidies originated from oocytes (29) and/or sperm (5).…”

Section: Discussionmentioning

confidence: 99%

“…Artificial oocyte activation (AOA) is a method of mimicking the physiological cell signaling mechanisms to activate oocytes, and calcium ionophore A23187 is the most widely used AOA method in humans (2)(3)(4)(8)(9)(10)(11)(12). Deliveries of healthy babies have been reported since AOA by calcium ionophore was adopted (2)(3)(4)(9)(10)(11)(12). A systematic review of published reports demonstrated acceptable outcomes of calcium ionophore use, particularly for couples in which ICSI alone yielded poor or no fertilization (11).…”

Section: Introductionmentioning

confidence: 99%

“…Deliveries of healthy babies have been reported since AOA by calcium ionophore was adopted (2)(3)(4)(9)(10)(11)(12). A systematic review of published reports demonstrated acceptable outcomes of calcium ionophore use, particularly for couples in which ICSI alone yielded poor or no fertilization (11). Recently, it has been reported that obstetric and neonatal malformations were not increased after ICSI and AOA (13).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Niu¹,

Ruan²,

Witz

et al. 2020

Front. Endocrinol.

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Fertilization failure is common in patients with round-headed sperm, a form of globozoospermia. Artificial oocyte activation is able to assist oocyte fertilization after sperm injection in these patients. Comparisons between oocyte fertilization with or without calcium ionophore have been reported in patients with round-headed sperm. However, no comparison has been reported between round-headed sperm injection followed by calcium ionophone activation and normal sperm injection. In this case report, half of oocytes from a patient were injected with her partner's round-headed sperm followed by calcium ionophore activation, and the other half of oocytes were injected with a donor sperm without calcium ionophore activation. The injected oocytes were cultured to examine fertilization, embryo development, and embryonic aneuploidies in the resulting blastocysts. The fertilization rate was lower in round-headed sperm injected oocytes (3/6) than that in donor sperm injected oocytes (5/6), but rates of blastocyst and aneuploidies were similar in the resulting embryos between the two groups. A euploid blastocyst resulted from round-headed sperm injection was transferred, and a healthy baby was delivered. These results indicate that calcium ionophore treatment can assist oocyte activation in patients with round-headed sperm, but its efficiency to activate oocytes is lower than that induced by a normal sperm injection. However, embryo development and chromosome integrity may not be affected by calcium ionophore treatment.

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Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis

Cited by 64 publications

References 37 publications

Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update

Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update

Current Topics in Assisted Reproduction in the Czech Republic

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

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