Does the rate of ribosomal RNA synthesis vary depending on the number of nucleoli in a nucleus?

Kurata, Shigeaki; Misumi, Yoshio; Sakaguchi, Bungo; Shiokawa, K.; Yamana, K.

doi:10.1016/0014-4827(78)90297-5

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…rRNA transcription is decreased in SUN1 depleted cells SUN1-depleted cells exhibited nuclei with fewer but larger nucleoli. Because structural alteration of the nucleolus is often linked to altered ribosomal biogenesis, [19][20][21][22][23][24] expression of the 45S precursor rRNA (prerRNA), which is very short lived and represents the rate of rRNA transcription, was quantified using real-time PCR. SUN1-depleted cells reproducibly exhibited a 40-60% decrease in pre-rRNA synthesis (Fig.…”

Section: Sun1 Depletion Affects the Distribution Of Nucleoli And Chromentioning

confidence: 99%

“…17,18 The enlarged nucleolar size typically reflects deregulation of rRNA transcription. [19][20][21][22][23][24] However, little is known about the molecular mechanism that induces the aberrant nucleolar morphologies and deregulates rRNA transcription.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23][24] However, the presence of enlarged nucleoli does not always correlate with activated rRNA synthesis, and the causal relationship between nucleolar enlargement and activated rRNA synthesis is unclear.…”

mentioning

confidence: 99%

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Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Matsumoto

Sakamoto

Matsumori

et al. 2016

Nucleus

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A supervised machine learning algorithm, which is qualified for image classification and analyzing similarities, is based on multiple discriminative morphological features that are automatically assembled during the learning processes. The algorithm is suitable for population-based analysis of images of biological materials that are generally complex and heterogeneous. Here we used the algorithm wndchrm to quantify the effects on nucleolar morphology of the loss of the components of nuclear envelope in a human mammary epithelial cell line. The linker of nucleoskeleton and cytoskeleton (LINC) complex, an assembly of nuclear envelope proteins comprising mainly members of the SUN and nesprin families, connects the nuclear lamina and cytoskeletal filaments. The components of the LINC complex are markedly deficient in breast cancer tissues. We found that a reduction in the levels of SUN1, SUN2, and lamin A/C led to significant changes in morphologies that were computationally classified using wndchrm with approximately 100% accuracy. In particular, depletion of SUN1 caused nucleolar hypertrophy and reduced rRNA synthesis. Further, wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. Our unbiased morphological quantitation strategies using wndchrm revealed an unexpected link between the components of the LINC complex and the morphologies of nucleoli that serves as an indicator of the malignant phenotype of breast cancer cells.

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Section: Sun1 Depletion Affects the Distribution Of Nucleoli And Chromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Matsumoto

Sakamoto

Matsumori

et al. 2016

Nucleus

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Nucleolar activity in the fusiform cambial cells of Abies balsamea (Pinaceae): effect of season and age

Mellerowicz¹,

Riding²,

Little³

1993

American J of Botany

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Nucleolar involvement in the regulation of the activity‐rest‐quiescence cycle of the vascular cambium was assessed by determining the seasonal variation in number, diameter, and volume of nucleoli in fusiform cells of Abies balsamea (L.) Mill. The cells were isolated from 1‐ and 19‐yr‐old cambia and stained with either silver nitrate or Feulgen + naphthol yellow‐S. The ability of fusiform cells to incorporate [5‐3H]‐uridine into nuclei and nucleoli was also determined. In the 1‐yr‐old cambium, the activity of the nucleoli, as evidenced by their diameter, total volume per cell, and intensity of staining with silver nitrate, exhibited two maxima during the year—a large one during cambial reactivation in April‐May and a small one during the rest‐quiescence transition in October. Incorporation of radiolabeled uridine at 20 C was low at the end of the active period and increased during the rest–quiescence transition, suggesting that the quiescent, but not the resting, cambium can rapidly resume nucleolar activity when the temperature is permissive. The number of nucleoli per cell varied between two and eight, and was higher during the dormant than the active period. The increase in number took place during the autumnal activity–rest–quiescence transition, when cambial cells were arrested in the G1 phase of the cell cycle. Similar seasonal changes in nucleolar morphology were observed in the 19‐yr‐old cambium. Nucleolar diameter and total nucleolar volume were larger in the 19‐yr‐old cambium than in the 1‐yr‐old cambium, whereas nucleolar number was lower. Th results suggest that repression of rRNA genes underlies the development of rest when the cambium will not produce new cells.

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Nucleolar Organiser Regions

Crocker¹

1990

Current Topics in Pathology

116

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Does the rate of ribosomal RNA synthesis vary depending on the number of nucleoli in a nucleus?

Cited by 9 publications

References 8 publications

Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Nucleolar activity in the fusiform cambial cells of Abies balsamea (Pinaceae): effect of season and age

Nucleolar Organiser Regions

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