Does the apoptosis pathway play a critical role in gonocyte transformation?

Tien, Melissa Yixin; Abeydeera, Sanduni Amaya; Cho, Hyun Jung; Sarila, Gulcan; Catubig, Angelique; Burton, Emily; Hutson, John M.; Li, Ruili

doi:10.1016/j.jpedsurg.2019.09.038

Cited by 5 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gonocyte-to-SSC transformation occurs soon after birth (2–6 days) in mice ( Law and Oatley, 2020 ) and 3–6 months in humans ( Hutson et al, 2013 ). A considerable number of gonocytes fail to migrate and undergo apoptosis ( Orwig et al, 2002 ), and the unmigrated cells often serve as the source of germ cell tumor formation in cryptorchid testes in humans ( Tien et al, 2020 ).…”

Section: Germline Development In Fetal and Neonatal Testismentioning

confidence: 99%

Functional Importance of Mini-Puberty in Spermatogenic Stem Cell Formation

Shima

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Primordial germ cells nesting in the fetal testis give rise to gonocytes. The gonocytes then transform into spermatogenic stem cells (SSCs) during the neonatal period and thereafter serve as a lifetime source of spermatogenesis. Therefore, gonocyte to SSC transformation is quite an important process that supports fertility in males. During the gonocyte to SSC transformation, morphological and transcriptomic changes sequentially occur and gonocytes migrate from the center to the peripheral region of the seminiferous tubules. However, extrinsic signals which trigger the transcriptomic changes as well as the migration are not yet fully clarified. Recent studies have drawn attention to the temporal activation of the hypothalamic-pituitary-gonadal axis during the neonatal stage which occurs concurrently with SSC formation. This phenomenon is called mini-puberty, and recent studies on human cryptorchid patients as well as animal models partially support the hypothesis that mini-puberty plays pivotal roles in gonocyte-to-SSC transformation. Focusing on this point, here, we aimed to discuss the latest knowledge on the importance of mini-puberty in spermatogenesis in this review.

show abstract

Section: Germline Development In Fetal and Neonatal Testismentioning

confidence: 99%

Functional Importance of Mini-Puberty in Spermatogenic Stem Cell Formation

Shima

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…It was suggested that GCNIS originates from gonocytes during embryonic development, as suggested by the morphological similarities between gonocytes and atypical cells present in the lesion [ 4 ], as well as by their expression of proteins such as POU domain class 5 transcription factor 1 (POU5F1), the transcription factor AP2γ (AP2γ), the tyrosine kinase receptor c-Kit (KIT), placental-like alkaline phosphatase (PLAP), sal-like protein 4 (SALL4), the homebox transcription factor Nanog (NANOG), and thy-1 cell surface antigen (THY1) [ 5 , 6 ]. Some reports show the persistence of gonocytes in pediatric CO patients [ 7 ], which did not mature into type A spermatogonia [ 8 ]. It was reported that a low proportion of CO patients older than one year of age may present the expression of at least one and up to five previously described proteins.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Patients with Cryptorchidism: Preliminary Search for an Expression Profile Related to That of Testicular Germ-Cell Tumors

García-Andrade,

Vigueras-Villaseñor,

Chávez-Saldaña

et al. 2023

Diagnostics

View full text Add to dashboard Cite

Cryptorchidism (CO) is a risk factor for the development of testicular germ-cell tumors (TGCT). This is supported by reports showing the persistence of gonocytes in CO patients. These cells are proposed to be related to the development of germ-cell neoplasia in situ (GCNIS), which is considered the precursor stage/lesion of TGCT. Therefore, it is proposed that some patients with CO could express some molecular markers related to TGCT. In this study, we analyzed testicular tissue samples from CO, TGCT, and controls. We determined the expression of POU5F1, PLAP, and KIT by immunohistochemistry and that of the hsa-miR-371-373 cluster, hsa-miR-367, and LATS2, PTEN, and IGFR1 genes by RT-qPCR. We then carried out a bioinformatic analysis to identify other possible candidate genes as tumor biomarkers. We found that 16.7% (2/12) of the CO patients presented increased expression of POU5F1, KIT, PLAP, hsa-miR-371-373, and hsa-miR-367 and decreased expression of LATS2 and IGF1R. Finally, the genes ARID4B, GALNT3, and KPNA6 were identified as other possible candidate tumor biomarkers. This is the first report describing the expression of the hsa-miR-371-373 cluster, hsa-miR-367, LATS2, and IGF1R in the testicular tissues of two CO patients with cells immune-positive to POU5F1, PLAP, and KIT, which is similar to what is observed in TGCT.

show abstract