Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?

Oliveira, M. E. F.; Cordeiro, Mabel Freitas; Ferreira, Roberta Machado; Souza, S. F.; Pieroni, J. S. P.; Rodrigues, Luiz Fernando; Fonseca, J. F. da; Vicente, Wilter Ricardo Russiano

doi:10.1590/s0103-84782012000600021

Cited by 16 publications

(14 citation statements)

References 16 publications

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“…Despite this, it is known that its presence affects the superovulatory response and is one of the factors related to the high variability in viable embryo production in sheep (Veiga‐Lopez et al, ). Oliveira et al () observed that supplementation with LH at the end of the gonadotrophic treatment increases the frequency of CLs and decreases the percentage of luteinized anovulatory follicles; however, the authors suggest that the effect of changing the FSH/LH ratio in superovulatory protocols at ovulation rate is controversial and seems to vary according to different breeds.…”

Section: Discussionmentioning

confidence: 99%

“…On the day of embryo collection (5 or 6 days after ovulation—Day 15), videolaparoscopy procedures were performed according to Oliveira et al () to evaluate ovarian structures (Figure ), luteinized anovulatory follicles (luteal structures ≥5 mm and lacking ovulatory stigmata; Bartlewski et al, ) and CLs. All visualized structures were quantified.…”

Section: Methodsmentioning

confidence: 99%

“…In small ruminants superovulatory protocols, the total doses of pFSH (Folltropin®, Bioniche, Canada) more frequently used are 256 mg (Oliveira et al, ), 215 mg (Sánchez‐Dávila et al, ) or 200 mg (Loiola Filho et al, ; Oliveira et al, ) while in cows good results are achieved with lower doses (100–133 mg) (Baruselli et al, ). Therefore, studies addressing the efficacy of lower doses of FSH in sheep are needed.…”

Section: Introductionmentioning

confidence: 99%

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Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Rodriguez

Maciel

Uscátegui

et al. 2018

Reprod Domestic Animals

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Contents The aim was to compare the early luteal development in ewes superovulated with different doses of pFSH. Twenty‐nine Santa Inês ewes received a progesterone device (CIDR®) for 8 days. Gonadotrophic treatment started on Day 6: G200 (control, n = 9, 200 mg); G133 (n = 10, 133 mg); and G100 (n = 10, 100 mg of pFSH). On Day 6, all females received eCG (300 IU). B‐mode and spectral Doppler ultrasonography were performed daily during the early luteal phase (Days 11–15) to monitor the development of corpora lutea (CLs; dimensions) and ovarian arteries indices. CLs were also classified as normal or prematurely regressed (PRCL) on Day 15 by videolaparoscopy. Ewes from G100 and G133 showed gradual increase in luteal diameter during the early luteal phase (p < 0.001), whereas G200 animals presented increase from Day 11 to Day 13, and then decrease on Days 14 and 15 (p < 0.001). The G200 females showed greater percentage of PRCL (45.20%) than those of the other groups (p < 0.001). The normal CLs number was greater in G100 than in G133 (p = 0.04), while the PRCL number was greater in G200 than in the other groups (p = 0.03). Resistive index (RI) was greater in G200 than in G100 (p = 0.02). RI was lower in Day 12 than Day 15 (p = 0.02). Pulsatility index (PI) was greater on Days 14 and 15 (p < 0.01). In conclusion, the lowest dose of pFSH (100 mg) can be considered sufficient for an efficient superovulatory response in sheep, producing better CLs development dynamic in early luteal phase and ovarian blood perfusion and smaller number of PRCL than the traditional (200 mg) pFSH dose.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Rodriguez

Maciel

Uscátegui

et al. 2018

Reprod Domestic Animals

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“…The multiple ovulation and embryo transfer (MOET) is a reproductive biotechnology facilitating genetic improvement in livestock species, whose commercial application has increased significantly in recent years (Bartlewski et al., ; Oliveira et al., ). The high variability and unpredictability of responses to hormonal ovarian stimulation is notably the major challenge preventing further increase in MOET efficiency and its more widespread use in sheep (Menchaca, Vilariño, Crispo, Castro, & Rubianes, ; Oliveira, ).…”

Section: Introductionmentioning

confidence: 99%

Assessing the usefulness of B‐mode and colour Doppler sonography, and measurements of circulating progesterone concentrations for determining ovarian responses in superovulated ewes

Oliveira

Ribeiro

Rodriguez

et al. 2018

Reprod Domestic Animals

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The main goal of this study was to assess the usefulness of two imaging modalities, namely the B-mode and colour Doppler sonography, and serum progesterone (P ) concentrations for determining the ovarian response in superovulated ewes. Twenty-four sexually mature Santa Inês ewes underwent the superovulatory treatment consisting of eight injections of porcine FSH (total dose of 200 or 133 or 100 mg; n = 8 ewes/total dose) given at 12-hr intervals and initiated 48 hr before CIDR (Pfizer Inc., Auckland, New Zealand) removal. Six days after natural mating, the ovaries of all donor ewes were visualized and examined with transrectal ultrasonography and then with videolaparoscopy to identify and enumerate corpora lutea (CL) and luteinized unovulated follicles (LUFs). Jugular blood samples were collected just prior to ovarian examinations. The total number of CL (r = .78 and 0.83, p < .0001) and LUFs (r = .74 and 0.90, p < .0001) enumerated using the B-mode and colour Doppler ultrasonographic technique, respectively, were correlated with that ascertained by videolaparoscopy. Circulating concentrations of P were related directly to the number of healthy CL (r = .73, p = .0002) and inversely to the number of prematurely regressing CL (r = -.46, p = .03), but the accuracy of predicting the number of short-lived CL with serum P concentrations was very poor. The present results indicate that ultrasonographic imaging and serum P measurements on the day of embryo recovery are useful indicators of total/normal CL numbers and both ultrasonographic techniques can be used to quantify LUFs in superovulated ewes.

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“…Programas de superovulação realizados com raças localmente adaptadas apresentaram médias de ovulação que variaram entre 7,4 e 13,5 (Cordeiro et al, 2003;Lopes Júnior et al, 2006;Oliveira et al, 2012). Uma boa resposta ovulatória está diretamente relacionada com um maior rendimento embrionário, portanto o protocolo utilizado neste estudo é uma ferramenta importante para a conservação ex situ de raças localmente adaptadas.…”

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Produção de embriões em ovinos Morada Nova e Somalis Brasileira

Brasil

Moreira

Silva

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?

Cited by 16 publications

References 16 publications

Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Assessing the usefulness of B‐mode and colour Doppler sonography, and measurements of circulating progesterone concentrations for determining ovarian responses in superovulated ewes

Produção de embriões em ovinos Morada Nova e Somalis Brasileira

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