Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos

Riggs, Ryan; Mayer, Jacob; Dowling-Lacey, Donna; Chi, T.F.; Jones, Estella; Oehninger, Sergio

doi:10.1016/j.fertnstert.2008.09.084

Cited by 107 publications

(75 citation statements)

References 24 publications

(29 reference statements)

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“…Therefore, it appears that the duration of cryo-storage will not affect the outcome of frozen embryos. Several studies carried out in humans indicated that long-term cryo-storage did not affect embryo cryo-survival and pregnancy outcome in the IVF or embryo donation program [31,32]. However, a previous study reported that increased rates in embryonic cell death were found associated with cryo-storage of indicates that the embryos developed to 8-cell stage in the group of cryo-storage for 90-92 days was significantly reduced compared with the group of cryostorage for 8-10 days and the control group, which were similar indicated by a .…”

Section: Discussionmentioning

confidence: 99%

“…To date, only several studies of live births have been reported resulting from long-term cryo-preserved human embryos at zygote stage or cleavage stage with a duration of 7.5 years, 8 years, 8.9 years and 12 years, respectively [34][35][36][37]. Based on an analysis of 11,768 cryo-preserved human embryos, recently Riggs et al [31] have reported that the "oldest" zygotes resulting in live births were stored for 9.2 years, and the "oldest" cleavage embryos resulting in live births was stored for 5 years, but no pregnancies resulted from embryos that had been cryo-preserved for more than 10 years. The results indicated that there are some negative effects of long-term cryo-storage on human embryo viability [31].…”

Section: Discussionmentioning

confidence: 99%

“…Based on an analysis of 11,768 cryo-preserved human embryos, recently Riggs et al [31] have reported that the "oldest" zygotes resulting in live births were stored for 9.2 years, and the "oldest" cleavage embryos resulting in live births was stored for 5 years, but no pregnancies resulted from embryos that had been cryo-preserved for more than 10 years. The results indicated that there are some negative effects of long-term cryo-storage on human embryo viability [31]. All those studies suggest that cryo-duration in liquid nitrogen may affect the outcome of embryo cryopreservation, and long cryoduration probably damages to the viability of embryos.…”

Section: Discussionmentioning

confidence: 99%

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Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Yan

Suzuki

et al. 2011

J Assist Reprod Genet

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Purpose To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming. Methods Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared. Result(s)The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4±7.9%) compared to that of the other two groups (97.4±3.0% and 98.0± 3.3%). The fertilization rate in the group of 180-182 days (66.6±22.0%) was also significantly reduced (P<0.05) compared with the groups of 8-10 days (92.2±10.8%) and 90-92 days (94.7±9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P<0.05) following long cryo-storage duration (72.1± 8.2%, 25.2±3.8% and 5.5±13.6%, respectively). Conclusion(s) The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Yan

Suzuki

et al. 2011

J Assist Reprod Genet

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“…Studies have demonstrated no correlation between embryo storage duration and the live birth rate [26], which is reassuring for oncology patients, given they often delay pregnancy to complete adjuvant chemotherapy and concerns over cancer recurrence [17].…”

Section: Introductionmentioning

confidence: 99%

Fifteen year follow-up of embryos cryopreserved in cancer patients for fertility preservation

Barcroft

Dayoub

Thong

2013

J Assist Reprod Genet

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Purpose Determine the outcome of embryo cryopreservation in female oncology patients Methods The outcomes of IVF/ICSI cycles in oncology patients over 15 years in a University Teaching Hospital. Results Forty-two oncology patients (mean 31.9±3.9 years) underwent embryo cryopreservation treatment (n=33 IVF, n=6 ICSI). Controlled ovarian stimulation with GnRH antagonist protocol (n=34; 81 %) yielded fewer oocytes than GnRH agonist protocol (n=8; 19 %) (9.4±6.3 vs. 15.3±8.9; p=0.04) respectively. There was no significant difference in mean (±SD) duration of ovarian stimulation (11.6±2.6 vs.10.6±2.7), median gonadotrophin dose (1950( vs. 1670, median day 5-6 oestradiol level (1124 vs.1129 pmol/l) or embryo yield (6.2±4.1 vs. 8.8± 4.3; p=0.07) between GnRH antagonist and agonist treatment cycles respectively. Thirty-nine patients cryopreserved embryos and three had their cycle cancelled. During this study period, of those who cryopreserved embryos, 5 patients underwent 9 frozen-thaw cycles (13 %), resulting in 2 live births (1 twin, 1 singleton, live birth rate 22 %). Six patients died (15 %), 3 conceived naturally (8 %) and 2 couples separated (5 %). Fourteen patients discarded their embryos (36 %). Twenty-two patients' (56 %) have embryos remaining in storage. Conclusions This study demonstrates that embryo cryopreservation in female oncology patients gives a satisfactory live birth rate. However, there are concerns regarding costeffectiveness, resulting from high disposal/non-usage of embryos, and further studies are required.

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“…Nevertheless, in 2008 the Practice Committee of American Society of Reproductive Medicine (ASRM) concluded that "Oocyte cryopreservation presently should be considered an experimental technique," as the data available on the efficacy of oocyte cryopreservation in relation to the length of storage are still limited [1,12]. Instead, the ASRM considers embryo cryopreservation a non-experimental technique [12], with a proven clinical outcome regardless of the length of storage [13,14]. Unfortunately, there are limited data concerning children born from long-term cryopreserved oocytes [1].…”

Section: Introductionmentioning

confidence: 99%

Live birth from oocytes cryopreserved with slow-freezing protocol and thawed after 6 years of storage

Sala

Capodanno²,

Valli³

et al. 2012

J Assist Reprod Genet

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Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos

Cited by 107 publications

References 24 publications

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Fifteen year follow-up of embryos cryopreserved in cancer patients for fertility preservation

Live birth from oocytes cryopreserved with slow-freezing protocol and thawed after 6 years of storage

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