Does prolactin induce apoptosis? Evidences in a prostate cancer in vitro model

Giuffrida, Domenica; Perdichizzi, Anna; Giuffrida, Maria Chiara; Vignera, Sandro La; D’Agata, Roberta; Vicari, Enzo; Calogero, Aldo E.

doi:10.1007/bf03346592

Cited by 12 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, opposite effects have been found on prostate cancer cell lines: rec-hPRL, induces apoptosis in LNCaP cells, but not in PC3 cells (44). This demonstrates that PRL can modulate different actions depending on the type of tumor.…”

Section: Discussionmentioning

confidence: 93%

A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production

et al. 2018

View full text Add to dashboard Cite

Abstract. Prolactin (PRL) is associated with different types of cancer, such as cervical cancer. Recombinant PRL has antiapoptotic effect on cervical cancer cells, and it can also induce cytokine production on macrophages. A 60 kDa variant of PRL is produced by cervical cancer cells. The aim of the present study was to evaluate this variant's bioactivity, to test its effect on cervical cancer cell apoptosis, and to assess its ability to induce cytokine production on THP-1 macrophages. First, 60 kDa PRL was isolated and used to stimulate Nb2 cells. Later, apoptosis was measured after exposure to 60 kDa PRL. Finally, cytokines were measured on THP-1 stimulated supernatants. Our results show that 60 kDa PRL increased Nb2 cell proliferation. Apoptosis was decreased after stimuli with 60 kDa PRL in cervical cancer cells. IL-1β and TNF-α are produced by THP-1 macrophages after stimuli. These results suggest that 60 kDa PRL produced by cervical cancer cells is able to reduce apoptosis in HeLa, SiHa and C-33A cells and induce IL-1β and TNF-α production by THP-1 macrophages.

show abstract

Section: Discussionmentioning

confidence: 93%

A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The disparity in the results can be influenced by the use of different culture techniques and conditions including the use of different clones of each cell line. One more study, also reported that PRL treatment had no effect on proliferation of LNCaP and PC3 prostate cancer cell lines but showed that PRL has a pro-apoptotic effect in the androgen responsive cell line LNCaP [41]. …”

Section: Discussionmentioning

confidence: 99%

High expression of prolactin receptor is associated with cell survival in cervical cancer cells

et al. 2013

View full text Add to dashboard Cite

BackgroundThe altered expression of prolactin (PRL) and its receptor (PRLR) has been implicated in breast and other types of cancer. There are few studies that have focused on the analysis of PRL/PRLR in cervical cancer where the development of neoplastic lesions is influenced by the variation of the hormonal status. The aim of this study was to evaluate the expression of PRL/PRLR and the effect of PRL treatment on cell proliferation and apoptosis in cervical cancer cell lines.ResultsHigh expression of multiple PRLR forms and PRLvariants of 60–80 kDa were observed in cervical cancer cell lines compared with non-tumorigenic keratinocytes evaluated by Western blot, immunofluorecence and real time PCR. Treatment with PRL (200 ng/ml) increased cell proliferation in HeLa cells determined by the MTT assay at day 3 and after 1 day a protective effect against etoposide induced apoptosis in HeLa, SiHa and C-33A cervical cancer cell lines analyzed by the TUNEL assay.ConclusionsOur data suggests that PRL/PRLR signaling could act as an important survival factor for cervical cancer. The use of an effective PRL antagonist may provide a better therapeutic intervention in cervical cancer.

show abstract

“…In conclusion, it is not clear whether prostate autocrine PRL expression is increased in BPH and so far there are not strong evidences supporting a role of this hormone in promoting BPH. Finally, it is relevant to report that there are some evidences suggesting a possible role of PRL on prostate cancer development (Giuffrida et al ., ).…”

Section: Benign Prostate Hyperplasia: Role Of Prolactinmentioning

confidence: 97%

Endocrine control of benign prostatic hyperplasia

et al. 2016

Self Cite

View full text Add to dashboard Cite

SUMMARYBenign prostatic hyperplasia (BPH) is the most common benign proliferative disease among aging men. Androgens play a key role in the development and growth of the male genital tract favoring differentiation and proliferation of stromal and epithelial cells of the prostate gland. It is known that growth factors play a crucial role in the cross-talk between stromal cells and epithelial cells. These factors, mainly secreted by stromal cells, act in an autocrine/paracrine manner to maintain prostate cellular homeostasis. A number of experimental studies support the interdependence between growth factors (IGF, FGF, TGF) and the steroid hormone milieu of the prostate. Alterations of these interactions may alter the balance between proliferation and cell death leading to the development of BPH. The onset of BPH is closely related to an inflammatory microenvironment. Chronic inflammation, which generally follows the acute inflammation because of infectious agents, is favored by hormonal or metabolic abnormalities. However, a close correlation between these mechanisms and metabolic or sexual hormones (androgen/estrogen ratio) alteration has been shown suggesting a key role of hypogonadism in the development of prostate inflammation. This review clear shows that the BPH pathogenesis and the subsequent onset of the lower urinary tract symptoms (LUTS) depends from different etio-pathogenetic factors whose mechanism of action remains to be evaluated.

show abstract

Does prolactin induce apoptosis? Evidences in a prostate cancer in vitro model

Abstract: This study indicates that PRL induces apoptosis in the androgen-responsive cell line LNCaP, whereas no effect was observed in the androgen-insensitive PC3 cell line. These findings suggest that androgen responsiveness may be required for PRL to be effective on prostatic cells.

Cited by 12 publications

References 19 publications

A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production

A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production

High expression of prolactin receptor is associated with cell survival in cervical cancer cells

Endocrine control of benign prostatic hyperplasia

Contact Info

Product

Resources

About