Does progesterone have protective effects on ovarian ischemia-reperfusion injury?

Abstract

Objective:The aim of the present study was to evaluate the effects of progesterone (PG) against ovarian ischemia-reperfusion (I/R) injury through the evaluation of biochemical and histopathologic parameters.Material and Methods:Twenty-one female Wistar albino rats were divided into three groups. Group 1: Sham; group 2: I/R; group 3: I/R+PG (8 mg/kg). PG was administered intraperitoneally to the rats in group 3, 30 minutes before a detorsion operation. Ovarian I/R injury was evaluated in serum and tissue by using biochemical parameters including malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index, neutrophil gelatinase-associated lipocalin (NGAL) and immunofluorescence staining by using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay.Results:Serum and tissue TOS levels were significantly lower in group 3 than in group 2. Tissue TAS levels were higher in group 3 than in group 2 (p<0.001). NGAL and MDA levels were similar between the groups. Histologic score, including vascular congestion, hemorrhage, polymorphonuclear neutrophils, and interstitial edema, was higher in group 2. Pre-treatment with PG decreased the score, but this difference was not statistically significant. The number of apoptotic cells was higher in group 2 than in groups 1 and 3. The TUNEL-positive cell number decreased with PG in group 3.Conclusion:Preoperative PG treatment might exert protective effects on ovarian I/R injury through its anti-apoptotic and antioxidative properties.

The rat ovaries after U-74389G process

“…11 Banu Güleç et al made the assumption that preoperative Progesterone (PG) treatment might exert protective effects on ovarian I/R injury through its anti-apoptotic and antioxidative properties; since serum and tissue TOS levels, histologic score including vascular congestion, hemorrhage, polymorphonuclear neutrophils, number of apoptotic cells and interstitial edema were significantly lower and tissue TAS levels were higher in treated group (p<0.001). 12 However, the PG pre-treatment decrease was not statistically significant. The present experiment shows that L attenuates the cytokine TNFα levels which is involved in systemic inflammation, is one of the cytokines that make up theacute phase reaction, stimulates phagocytosis and the production of IL-1 oxidants and thus attenuates all of these procedures by 4.69% [+8.40%] (p-value=0.5749).…”

The Rat Ovaries after Erythropoietin Process

“…Marwa M M Refaie et al attributed 15 protective effect of Pioglitazone (PIO), anti-inflammatory, anti-apoptotic and anti-oxidant properties; since it reduced non significantly the induced increased ovarian tissue levels of MDA, NO x , gene expressions of p53, TNFα, iNOS, GSH, HO-1 levels, PPARγ, eNOS gene expressions and the marked ovarian edema, hemorrhage and congestion associated with cell injury in an OIR experiment. Banu Güleç et al made 16 the assumption that preoperative Progesterone (PG) treatment might exert non-significant protective effects on OIR injury through its anti-apoptotic and antioxidative properties; since serum and tissue TOS levels and histologic scores were significantly lower and tissue TAS levels were higher in treated group (p<0.001).…”

Can ovarian aging be delayed by pharmacological strategies?

“…A recent study suggested that progesterone alone may protect ovarian function from ischemia-reperfusion injury through its anti-apoptotic and antioxidative properties [123]. More researches are still needed for the role of progesterone in ovarian function.…”