Does plasma nitrite determination by the Griess reaction reflect nitric oxide synthesis?

Nitric oxide and reactive species are modulated in the polyphenol-induced ductus arteriosus constriction in pregnant sheep

“…Nitric oxide (NO) is a reactive nitrogen species (RNS) generated from the endothelial nitric oxide synthase and acting as a signaling molecule, especially leading to vasodilatation, neurotransmission and inflammation . The NO radical has a very short serum half‐life and reacts with circulating reactive species to produce different stable inorganic metabolites, such as nitrites and nitrates; thus, nitrite/nitrate (NO x ) levels are often used as a measure of NO production.…”

“…36 Nitric oxide (NO) is a reactive nitrogen species (RNS) generated from the endothelial nitric oxide synthase and acting as a signaling molecule, especially leading to vasodilatation, neurotransmission and inflammation. 41 The NO radical has a very short serum half-life and reacts with circulating reactive species to produce different stable inorganic metabolites, such as nitrites and nitrates 42 ; thus, nitrite/nitrate (NO x ) levels are often used as a measure of NO production. Polyphenols present anti-inflammatory activities, 43,44 and nitric oxide production generates free radicals by nitric oxide synthase 45 ; therefore, NO inhibition by polyphenols is also congruent with their antioxidant activities.…”

Health benefits of subcutaneous zinc edetate and diphenyl diselenide in calves during the weaning period

“…NItRAte/NItRIte leVelS Nitric oxide levels were determined by indirectly as levels of NOx (nitrate/nitrite) using a colorimetric reaction, considered the standard technique for measuring NO 2 levels (Dusse et al 2005).…”

Coffee and Maillard products activate NF‐κB in macrophages via H2O2 production

“…The Griess reaction was used to measure nitrite spontaneously formed from NO [16]. A quantity of 3610 4 NR8383 cells/well was cultivated in a 96-well plate for 4 h. The cells were stimulated with the Maillard reaction mixture in medium (rib-lys 30 min) for 3 h. As a positive control, INF-c was added to the cells in medium in the indicated concentrations.…”