Does Long-Term Use of Silver Nanoparticles Have Persistent Inhibitory Effect on<i>H. pylori</i>Based on Mongolian Gerbil’s Model?

Kuo, Chao‐Hung; Lu, Chien‐Yu; Yang, Yuan-Chieh; Chin, Chieh; Weng, Bi-Chuang; Liu, Chung‐Jung; Chen, Yen-Hsu; Chang, Lin‐Li; Kuo, Fu‐Chen; Wu, Deng‐Chyang; Su, Hong-Lin

doi:10.1155/2014/461034

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…Besides, H. pylori infection may induce extragastric diseases, making it difficult to determine the health status of experimental gerbils [ 28 ]. Though H. pylori detection before replicating the H. pylori -infected model in gerbils is necessary, we found few studies that used H. pylori -free gerbils or detected the natural infection before challenge with H. pylori [ 10 , 11 ]. However, we cannot confirm whether the animal had been infected after modelling for a long time unless the animal was sacrificed, which affected the efficiency of modelling and did not conform to the “3R principle” (reduce, refine, and replace).…”

Section: Discussionmentioning

confidence: 99%

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Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Zhang

Wang

et al. 2022

BioMed Research International

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Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the 13C-urea breath test, which can detect Hp before modeling Hp infection in Mongolian gerbils. We designed a gas collection equipment for gerbils. Hp nested PCR was also performed on gastric fluid, gastric mucosa, duodenal contents, and faeces of gerbils challenged with Hp. Conventional Hp detection methods, including rapid urease assay and immunohistochemistry, were compared. Moreover, we assessed the natural infection of Hp in 135 gerbils that had never been exposed to Hp artificially from the major laboratory gerbil groups in China. In 10 Hp infected gerbils, the positive detection results were 100%, 100%, 90%, and 10% in gastric fluid, gastric mucosa, duodenal contents, and faeces with nested PCR, respectively. A rapid urease test performed on gastric mucosa showed that all animals were infected with Hp. Immunohistochemical detection and bacteria culture of gastric mucosa samples that were positive by the nested PCR method also confirmed the presence of Hp. 9% (3/35) and 6% (2/31) natural infection rates were found in conventional gerbil groups from the Capital Medical University and Zhejiang Laboratory Animal Center. In conclusion, we established two noninvasive Hp detection methods that can be performed before modelingHp infection, including the gastric fluid nested PCR method and the 13C-urea breath test.

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Section: Discussionmentioning

confidence: 99%

“…Nevertheless, in many studies, H. pylori -free gerbils were not used or gerbils were not evaluated for natural infection before infection with H. pylori . It impacts the reliability and consistency of previous studies [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 98%

Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Zhang

Wang

et al. 2022

BioMed Research International

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“…In the vast majority of the studies, spherical silver nanoparticles were used, obtained from different extracts, having various sizes: A. priceps , 5–30 nm [ 125 ]; S. xanthocarpum , 4–18 nm [ 32 , 126 ]; T. vernicifluum , 4–20 nm [ 127 ]; C. longa [ 128 ] and A. nilotica , 22–66 nm [ 124 ]; P. putida , 6–16 nm [ 35 ]; A. calamus , 5–60 nm [ 129 ]; A. nilotica , 22–55 nm [ 130 ]. The most common effect on the bacterial colonies was inhibition of growth, which was presented in six of the papers [ 124 , 125 , 127 , 128 , 131 , 132 , 133 , 134 ], followed by activation of reactive oxygen species production [ 130 , 132 ], inactivation of H. pylori urease [ 131 , 135 ], bacterial DNA fragmentation and consecutive loss of viability [ 130 ], damage to the bacterial cellular membrane [ 132 ], and activation of antioxidant activity [ 136 ].…”

Section: The Antibacterial Mechanism Of Silver Nanoparticles: An Exte...mentioning

confidence: 99%

“…There are few in vivo studies addressing the antibacterial effect of AgNPs against H. pylori (summarized in Table 3 ). One relevant study was performed in vivo by Kuo et al [ 136 ] on a batch of 8-week-old gerbils with bodyweight between 30 and 40 g which were fed with AgNp/clay complex at 0.1% of their bodyweight and another batch that was fed with the same complex at 1% of their bodyweight. In a study in 2020, Al-Bahrani et al showed a good inhibition of growth using an extract of A. bisporus [ 138 ].…”

Section: The Antibacterial Mechanism Of Silver Nanoparticles: An Exte...mentioning

confidence: 99%

Helicobacter Pylori-Induced Gastric Infections: From Pathogenesis to Novel Therapeutic Approaches Using Silver Nanoparticles

et al. 2022

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Helicobacter pylori is the first formally recognized bacterial carcinogen and the most important single digestive pathogen responsible for the induction of gastroduodenal diseases such as gastritis, peptic ulcer, and, finally, gastric neoplasia. The recently reported high rates of antimicrobial drug resistance hamper the current therapies of H. pylori, with therapeutic failure reaching up to 40% of patients. In this context, new treatment options and strategies are urgently needed, but the successful development of these new therapeutic tools is conditioned by the understanding of the high adaptability of H. pylori to the gastric acidic environment and the complex pathogenic mechanism. Due to several advantages, including good antibacterial efficiency, possible targeted delivery, and long tissular persistence, silver nanoparticles (AgNPs) offer the opportunity of exploring new strategies to improve the H. pylori therapy. A new paradigm in the therapy of H. pylori gastric infections using AgNPs has the potential to overcome the current medical limitations imposed by the H. pylori drug resistance, which is reported for most of the current organic antibiotics employed in the classical therapies. This manuscript provides an extensive overview of the pathology of H. pylori-induced gastritis, gastric cancer, and extradigestive diseases and highlights the possible benefits and limitations of employing AgNPs in the therapeutic strategies against H. pylori infections.

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“…as well as oldness and weak-ness [7]. Therefore, the main principles for insomnia treatment in Mongolian medicine are inhibition of "Haoyi" and regulation of "Sangen" [8]. The action of warm needles on the appropriate acupoints can properly alleviate "Haoyi" and balance "Sangen" for the purpose of insomnia treatment.…”

Section: Introductionmentioning

confidence: 99%

Research on the Mechanism of Mongolian Medical Warm Acupuncture in Alleviating Insomnia by Increasing the BCL-2/BAX Ratio and Decreasing the Hormones Related to the Central HPA Axis of the Stress Response

Wang

et al. 2017

Phys Med Rehab Kuror

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Background Mongolian medical warm acupuncture is efficacious in treatment of insomnia but the mechanism still remains unclear. There is no research on the effect on the changes in expression of mRNA in the receptors of 5HT, DA, 5HT1α, 5HT2α, and DRD2 and the levels of hormones related to the central HPA axis in the stress response. Objective To further explain the mechanism of Mongolian medical warm acupuncture in insomnia treatment and its role in protecting cerebral neurons, elaborate the effect of Mongolian medical warm acupuncture on the changes in expression of 5HT, DA, 5HT1α, and 5HT2α at different sites in the brains of the PCPA-induced insomnia model rats and the levels of hormones related to the central HPA axis in the stress response. Method 72 Wistar rats were randomly divided into blank group, model group, Mongolian medical warm acupuncture group, and diazepam group of 18 each. The rats were intraperitoneally injected with PCPA (400 mg/kg) for 2 consecutive days to induce insomnia. The sleep latency and sleep duration before and after treatment were observed. The content of 5HT in the hypothalamus and hippocampus was measured with the ELISA method. The content of mRNA in the 5HT1A and 5HT2A receptors in the hypothalamus and hippocampus with the qPCR technique. The content of DA in the hypothalamus and corpus striatum was determined with the ELISA method. The content of ELISA in the hypothalamus and the content of ACTH in the serum of the insomnia rats were determined with the ELISA method. The changes in content of the BCL-2 and BAX proteins in the hippocampus were observed with the immunohistochemical method. Result PCPA intraperitoneal injections were able to prolong the sleep latency of the rats and shorten the sleep duration (P<0.05) when compared with the blank group. Mongolian medical warm acupuncture was able to alleviate the tendency of decreasing weight gain arising from insomnia (P<0.05). Mongolian medical warm acupuncture was able to decrease the levels of CRH and ACTH in the hormones related to central HPA axis in the stress response (P=0.017, P=0.015) and alleviate the overexciting state of the HPA axis arising from insomnia. Mongolian medical warm acupuncture group was able to up-regulate the expression of the BCL-2 protein in the hippocampus, down-regulate the expression of the BAX protein, and increase the BCL-2/BAX ratio (P=0.011). Mongolian medical warm acupuncture was able to protect the hippocampal neurons by changing the content of the apoptosis factors (P=0.024). Conclusion Mongolian medical warm acupuncture decreases the levels of CRH and ACTH in the hormone related to the HPA axis, up-regulates the expression of the BCL-2 protein in the hippocampus, and down-regulates the expression of the BAX protein for improving rat insomnia by influencing the expression of 5HT1A and 5HT2A receptors in the hypothalamus and hippocampus.

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Does Long-Term Use of Silver Nanoparticles Have Persistent Inhibitory Effect onH. pyloriBased on Mongolian Gerbil’s Model?

Cited by 5 publications

References 27 publications

Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Helicobacter Pylori-Induced Gastric Infections: From Pathogenesis to Novel Therapeutic Approaches Using Silver Nanoparticles

Research on the Mechanism of Mongolian Medical Warm Acupuncture in Alleviating Insomnia by Increasing the BCL-2/BAX Ratio and Decreasing the Hormones Related to the Central HPA Axis of the Stress Response

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