Does Immunohistochemistry Allow Easy Detection of Lymphatics in the Optic Nerve Sheath?

Killer, Hanspeter E.; Jaggi, Gregor P.; Miller, Neil R.; Meyer, Peter

doi:10.1369/jhc.2008.950840

Cited by 45 publications

(34 citation statements)

References 30 publications

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“…However, a larger number of eyes from young children need to be analyzed to confirm this observation. We also failed to find lymphatics/D2-40 positive structures in the ciliary body as was reported by Yucel et al (2009) but could confirm podoplanin expression in the optic nerve sheaths which was interpreted as labeling of lymphatic vessels in the dura mater of the optic nerve and as a nonvascular pattern in the arachnoid mater by others Killer et al, 2008). The latter in particular suggests that podoplanin is not a selective marker for lymphatics (Killer et al, 2008).…”

Section: Expression Of Podoplanin In Our Cohort In Comparison With Thsupporting

confidence: 64%

“…We also failed to find lymphatics/D2-40 positive structures in the ciliary body as was reported by Yucel et al (2009) but could confirm podoplanin expression in the optic nerve sheaths which was interpreted as labeling of lymphatic vessels in the dura mater of the optic nerve and as a nonvascular pattern in the arachnoid mater by others Killer et al, 2008). The latter in particular suggests that podoplanin is not a selective marker for lymphatics (Killer et al, 2008). It seems worth noting that we found a non-vascular, non-lymphatic pattern of podoplanin labeling also in the suprachoroidal space, the arachnoid/optic nerve sheaths (and also the meninges (Nakamura et al, 2006)), and the trabecular meshwork with the latter two being involved in fluid circulation (liquor resp.…”

Section: Expression Of Podoplanin In Our Cohort In Comparison With Thsupporting

confidence: 57%

“…Similar to the nonvascular staining reaction in arachnoid mater that was attributed to the artificial detachment of the arachnoid layer (Killer et al, 2008), this reaction is more likely to be explained by accumulation of antibody in an artificial slit (e.g. the suprachoroidal space) that occurs by shrinkage of the tissue and detachment of the choroid from the underlying sclera or a genuine immunoreactivity of the suprachoroidal space.…”

Section: Implications On the Ongoing Discussion Regarding The Presumementioning

confidence: 85%

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Expression of the lymphatic marker podoplanin (D2-40) in human fetal eyes

Herwig

Münstermann

Klarmann‐Schulz

et al. 2014

Experimental Eye Research

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Section: Expression Of Podoplanin In Our Cohort In Comparison With Thsupporting

confidence: 64%

Section: Expression Of Podoplanin In Our Cohort In Comparison With Thsupporting

confidence: 57%

Section: Implications On the Ongoing Discussion Regarding The Presumementioning

confidence: 85%

See 1 more Smart Citation

Expression of the lymphatic marker podoplanin (D2-40) in human fetal eyes

Herwig

Münstermann

Klarmann‐Schulz

et al. 2014

Experimental Eye Research

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“…In fact, the existence of orbital lymphatic vessels has been debated due to the lack of proof of their existence in the orbital fat and extraocular muscles. Only recently has it been shown that a few lymphoid capillaries are present in the arachnoid and dura mater of the intraocular part of the optic nerve (Fogt et al 2004;Gausas et al 2007;Killer et al 2008). Therefore, it is speculated that the main supply of lymphocytes to the orbit comes from the orbital part of the lacrimal gland if needed, where lymphatic cells are abundant (Dickinson & Gausas 2006).…”

Section: Immunology Of the Orbitmentioning

confidence: 99%

Diffuse large B‐cell lymphoma and mantle cell lymphoma of the ocular adnexal region, and lymphoma of the lacrimal gland: An investigation of clinical and histopathological features

Rasmussen

2013

Acta Ophthalmologica

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Diffuse large B‐cell lymphoma (DLBCL) and mantle cell lymphoma (MCL) constitute two distinct subtypes of non‐Hodgkin lymphoma (NHL) associated with considerable morbidity and mortality.Marked diversities with regard to molecular biology and clinical features are recognized in different subsets of the two lymphomas. Because these differences could be related to the location of the lymphoma, it is of interest to investigate the clinical and histopathological features of DLBCL and MCL involving the ocular adnexal region (i.e. the orbit, eyelids, conjunctiva, lacrimal gland and lacrimal sac). Similarly, the lacrimal gland is the only glandular structure within the orbit. Because the lacrimal gland represents an important part of the immunological system, it is of interest to investigate lymphomas involving this location with regard to clinical and histological characteristics.Purpose: To characterize the clinical and histopathological features of Danish patients with DLBCL of the ocular adnexal region between 1980 and 2009 and of Danish ocular adnexal MCL patients from 1980 to 2005. Furthermore, the aim of this PhD was to review all specimens from patients with lymphoma of the lacrimal gland in Denmark between 1975 and 2009 to determine the distribution of lymphoma subtypes of the lacrimal gland and to describe the clinicopathological features of these patients.Results: A total of 34 patients with DLBCL and 21 with MCL of the ocular adnexal region were identified. Twenty‐seven patients had lacrimal gland lymphoma, including four DLBCLs and three MCLs from studies I and II.Elderly patients predominated in all three groups, with median ages of 78, 75 and 69 years in the DLBCL, the MCL and the lacrimal gland lymphoma groups, respectively. MCL patients had a preponderance of males, whereas females prevailed among lacrimal gland lymphoma patients. The orbit was the most common site of involvement in DLBCL and MCL. Most DLBCL patients had unilateral involvement, while MCL patients had a high frequency of bilateral involvement. Similarly, localized lymphoma was relatively frequently seen in DLBCL patients in contrast to the predominance of disseminated lymphoma in the MCL group.The majority of lacrimal gland lymphomas were low grade, and the distribution of subtypes was as follows: extranodal marginal zone lymphoma, 10 (37%); follicular lymphoma, 5 (19%); DLBCL, 4 (15%); MCL, 3 (11%); chronic lymphocytic leukaemia/small lymphatic lymphoma, 2 (7%); and unclassified B‐cell lymphoma, 3 (11%).The overall survival rates at 3 and 5 years for the entire study group of DLBCL were 42% and 20%, whereas 58% and 22% of MCL patients were alive 3 and 5 years after the time of diagnosis. The 5‐year overall survival rate of lacrimal gland lymphoma patients was 70%.Concordant bone marrow involvement and the International Prognostic Index score were predictive factors for the overall survival in the DLBCL group in Cox regression analysis. Rituximab‐containing chemotherapy was associated with an improved survival rate in MCL patients.Conclusions: Diffuse large B‐cell lymphoma and MCL involving the ocular adnexal region and lymphoma of the lacrimal gland are prevalent among elderly patients. The overall prognosis in DLBCL and MCL was poor, whereas the prognosis for lacrimal gland lymphoma patients was relatively good. Concordant bone marrow involvement and the International Prognostic Index score were independent predictive factors for mortality in the DLBCL group. Chemotherapy containing rituximab significantly improved survival in the MCL group.

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“…This thin intralobular lymphatic network may play a key pathophysiological role in a wide variety of alveolar and interstitial lung diseases and requires further investigation. LYMPHATIC VESSELS are present in almost all tissues except for avascular structures and rare vascularized organs such as brain and retina, although they have been recently described in the optic nerve sheath (Killer et al 2008). They constitute a one-way open-ended network running alongside the blood microvasculature.…”

mentioning

confidence: 99%

Intralobular Pulmonary Lymphatic Distribution in Normal Human Lung Using D2-40 Antipodoplanin Immunostaining

Kambouchner

Bernaudin

2009

J Histochem Cytochem.

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It has been assumed for a long time that except for limited areas close to respiratory bronchioles or their satellite arteries, there is no evidence of lymphatic vessels deep in the pulmonary lobule. An immunohistochemical study using the D2-40 monoclonal antibody was performed on normal pulmonary samples obtained from surgical specimens, with particular attention to the intralobular distribution of lymphatic vessels. This study demonstrated the presence of lymphatics not only in the connective tissue surrounding the respiratory bronchioles but also associated with intralobular arterioles and/or small veins even less than 50 μm in diameter. A few interlobular lymphatic vessels with a diameter ranging from 10 μm to 20 μm were also observed further away, in interalveolar walls. In conclusion, this study, using the D2-40 monoclonal antibody, demonstrated the presence of small lymphatic channels within the normal human pulmonary lobules, emerging from interalveolar interstitium, and around small blood vessels constituting the paraalveolar lymphatics. This thin intralobular lymphatic network may play a key pathophysiological role in a wide variety of alveolar and interstitial lung diseases and requires further investigation. (J Histochem Cytochem 57:643–648, 2009)

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Does Immunohistochemistry Allow Easy Detection of Lymphatics in the Optic Nerve Sheath?

Cited by 45 publications

References 30 publications

Expression of the lymphatic marker podoplanin (D2-40) in human fetal eyes

Expression of the lymphatic marker podoplanin (D2-40) in human fetal eyes

Diffuse large B‐cell lymphoma and mantle cell lymphoma of the ocular adnexal region, and lymphoma of the lacrimal gland: An investigation of clinical and histopathological features

Intralobular Pulmonary Lymphatic Distribution in Normal Human Lung Using D2-40 Antipodoplanin Immunostaining

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