Does exposure to reduced pH and diclofenac induce oxidative stress in marine bivalves? A comparative study with the mussel Mytilus galloprovincialis and the clam Ruditapes philippinarum

Munari, Marco; Matozzo, Valerio; Gagné, François; Chemello, Giulia; Riedl, Verena; Finos, Livio; Pastore, Paolo; Badocco, Denis; Marin, María Gabriella

doi:10.1016/j.envpol.2018.05.005

Cited by 65 publications

(25 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These may be due to a rapid and efficient neutralization of ROS by antioxidants to preserve a cellular redox balance and to prevent potential damages that they may exert altering sperm function. This is also supported by a previous report demonstrating that in M. galloprovincialis seawater acidification exposure induced an increase of antioxidant defenses and, consequently, this species results more resistant to reduced seawater pH (Munari et al, 2018).…”

Section: Discussionsupporting

confidence: 89%

Sperm Motility Impairment in Free Spawning Invertebrates Under Near-Future Level of Ocean Acidification: Uncovering the Mechanism

et al. 2020

View full text Add to dashboard Cite

Ocean acidification (OA) refers to the decrease in ocean water pH resulting from the increasing absorption of atmospheric CO 2. This will cause changes in the ocean's carbonate chemistry system with a resulting impact on reproduction of marine organisms. Reproduction is the fundamental process that allows the conservation of the species; in free-spawning marine invertebrates, this process is highly sensitive to changes in seawater quality and chemistry. To date, the majority of the studies concerned OA effects on reproduction has been focused on embryo and larval development. Despite several evidence for the impairment of reproductive success by environmental perturbations through altering gamete quality, sperm physiological responses to OA are poorly investigated. In this study, we evaluated the effects of exposure to acidified seawater (AcSW) (pH 7.8), which approximate the predicted global averages for oceanic surface waters at the end of this century, on sperm quality of the mussel Mytilus galloprovincialis and the ascidian Ciona robusta by evaluating several endpoints, such as motility, vitality, mitochondrial activity, oxidative state, and intracellular pH (pH i). Following sperm exposure to AcSW, the percentage of motile spermatozoa, mitochondrial activity and pH i decreased in comparison to the current seawater pH of 8.1, whereas vitality and oxidative state were unaffected by the low external pH in both the species. In broadcast spawners, a relationship between sperm intracellular pH and the initiation of motility are well known. Spermatozoa are immotile in the testes and motility is induced after the spermatozoa are released into seawater; the alkaline pH of seawater, in fact, increases the pH i activating motility and mitochondrial respiration. The results of this study suggest that the lowering of seawater pH as predicted to occur for 2100, through the inhibition of pH i increase, prevent sperm motility activation. Sperm motility is a key determinant of fertilization success; consequently, a corresponding drop in fertilization success would be expected with important implications for the fitness and the survival of marine invertebrates.

show abstract

Section: Discussionsupporting

confidence: 89%

Sperm Motility Impairment in Free Spawning Invertebrates Under Near-Future Level of Ocean Acidification: Uncovering the Mechanism

et al. 2020

View full text Add to dashboard Cite

show abstract

“…2016; Freitas et al, 2017;Lannig et al, 2010;Matoo et al, 2013;Moreira et al, 2018;Munari et al, 2018). In the scenario of global warming, aquatic organisms are subjected to water temperature fluctuations that may alter their thermal tolerances.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The effect of temperature on Triclosan and Lead exposed mussels

Pirone

Coppola

Pretti

et al. 2019

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

Interest on the effects of emerging contaminants over aquatic organisms has increased in the last years. Nonetheless, the toxic action of classical natural and anthropogenically-driven metals has also to be monitored, especially because they reflect real environmental situations. For that, in the present study we focused on the effects on the marine mussel Mytilus galloprovincialis of the personal care product Triclosan (TCS) and Lead (Pb), as toxic metal, under separate and co-exposure situations at environmentally relevant concentrations: TCS (1 µg/L) and Pb (50 µg/L). The consideration of an additional factor such as an increase in ambient temperature was also included to provide a forecasted scenario of climate change: from the ambient temperature at actual conditions (17ºC) to a predicted warming situation (22ºC). Water chemical characterization and some physical properties and bioaccumulation of TCS and Pb in mussels at the end of the experiment (28 days) was considered. The parameters followed up comprise the energy related system production (electron transport system) and glycogen and protein reserves. Antioxidant enzymatic defenses towards reactive oxygen species (ROS) and the consequences of ROS damage over endogenous lipids (LPO) and proteins (PC). Overall the results suggested only occasional/particular responses to chemical exposures at 17ºC whereas at 22ºC the detoxification machinery was set up and this prevented the occurrence of LPO. Nonetheless, PC formation occurred under Pb and TCS+Pb co-exposure at the highest temperature. Due to the complexity of the study: 4 chemical conditions, 2 temperatures and 10 biomarkers considered, a principal component ordination (PCO) analysis was included. The results of this integrative analysis confirmed a clear effect of the temperature, more responsiveness to drugs at 22ºC and in all likelihood due to Pb presence.

show abstract

“…galloprovincialis. Another recent study from Munari et al (2018) The use of biomarkers indicative of sublethal responses that are induced by PPCPs and temperature in terms of metabolic and oxidative stress related activities in bivalves has already been proven to be adequate. Metabolic capacity was evaluated by measuring the electron transport system (ETS) activity, as it had previously been shown that this parameter responded to warming conditions (Le Moullac et al, 2007;Freitas et al, 2017a; and the presence of pollutants (Andrade et al, 2018;Freitas et al, 2018;Monteiro et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…presence of PPCPs in aquatic ecosystems worldwide and their toxic effects on inhabiting organisms has been previously demonstrated. Recently, special attention has been given to the impact that environmental parameters, such as those related to climate change, have on PPCPs toxicity in aquatic invertebrates(Gonzalez-Ortegon et al, 2013;Munari et al, 2018; Serra-Compte et al, 2018). Despite these advances, current understanding of the effect of temperature on PPCP toxicity is still limited.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The influence of temperature on the effects induced by Triclosan and Diclofenac in mussels

Freitas

Coppola

Costa

et al. 2019

Science of The Total Environment

View full text Add to dashboard Cite

Little is known about the consequences of exposure to pharmaceuticals and personal care products (PPCPs) in elevated temperatures associated with climate change. To increase the knowledge on this topic, Mytilus galloprovincialis mussels were exposed to 1.0 µg/L of either the antimicrobial Triclosan (TCS) or the pharmaceutical drug Diclofenac (DIC), at control (17ºC) and 4ºC raised (21ºC) temperatures for 28 days.Triclosan and DIC concentrations in the water and tissues were subsequently measured and related to biomarker responses including: energy metabolism (electron transport system (ETS) activity, glycogen and protein reserves), oxidative stress markers, glutathione balance between the reduced and the oxidised form (GSH/GSSG), and damage to proteins and lipids. Mussels responded to the increase in temperature and drug exposure by lowering their metabolic rate (decreased ETS), increasing their endogenous reserves and antioxidant defences, thus preventing oxidative stress damage, with the exception of DIC exposure at the higher temperature.In all cases, GSH/GSSG ratio was reduced in detriment of the antioxidant form at both PPCPs exposures and elevated temperature with no additive effect due to combined stressors. Overall, either drug exposure or increased temperature could compromise the ability of mussels to withstand further insults.

show abstract

Does exposure to reduced pH and diclofenac induce oxidative stress in marine bivalves? A comparative study with the mussel Mytilus galloprovincialis and the clam Ruditapes philippinarum

Cited by 65 publications

References 85 publications

Sperm Motility Impairment in Free Spawning Invertebrates Under Near-Future Level of Ocean Acidification: Uncovering the Mechanism

Sperm Motility Impairment in Free Spawning Invertebrates Under Near-Future Level of Ocean Acidification: Uncovering the Mechanism

The effect of temperature on Triclosan and Lead exposed mussels

The influence of temperature on the effects induced by Triclosan and Diclofenac in mussels

Contact Info

Product

Resources

About