Does Escherichia coli have pathogenic potential at a low level of bacteriuria in recurrent, uncomplicated urinary tract infection?

Naboka, Y.L.; Mavzyiutov, Ayrat R.; Kogan, M. I.; Гудима, И А; Иванов, С. Н.; Naber, Kurt G.

doi:10.1016/j.ijantimicag.2020.105983

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…However, concerns about the suitability of this threshold for different kinds of UTI (e.g., urethritis, cystitis, and pyelonephritis) were raised ( Stamm et al, 1982 ; Sathiananthamoorthy et al, 2019 ). Naboka et al (2020) showed Enterobacteriaceae from the urine of women patients with RUTI at 10 2 CFU/ml had a wide spectrum of virulence factor genes, which may play a role in RUTI. However, the bacteria of 10 2 CFU/ml could not be detected by SUC.…”

Section: Discussionmentioning

confidence: 97%

Differential Urinary Microbiota Composition Between Women With and Without Recurrent Urinary Tract Infection

Huang

Zheng

et al. 2022

Front. Microbiol.

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BackgroundRecurrent urinary tract infection (RUTI) is common and burdensome in women. Due to the low concentration or slow-growing of uropathogens in RUTI, standard urine cultures (SUCs) are often negative. Next-generation sequencing (NGS) of bacterial 16S rRNA gene is more sensitive and could be used to reveal the differential microbiota between patients with RUTI and asymptomatic controls.MethodsWomen (aged ≥ 18 years) with clinically diagnosed RUTI with negative SUC and age-matched women asymptomatic controls with normal urinalysis were enrolled. Their midstream voided urine specimens were collected and processed for NGS (Illumina MiSeq) targeting the bacterial 16S rRNA gene V3-V4 region. The dataset was clustered into operational taxonomic units (OTUs) using QIIME. Taxonomic analysis, alpha diversity, beta diversity, multivariate statistical analysis, and linear discriminant analysis effect size (LEfSe) for differential analysis were performed and compared between patients with RUTI and asymptomatic controls.ResultsA total of 90 patients with RUTI and 62 asymptomatic controls were enrolled in this study. Among them, 74.4% (67/90) and 71.0% (44/62) were successfully amplified and sequenced their bacterial 16S rRNA gene. In the alpha diversity analysis, the chao1 index and observed species index were significantly lower in the RUTI group than in the control group (P = 0.015 and 0.028, respectively). In the beta diversity analysis, there was a significant difference between the 2 groups [Analysis of similarities (ANOSIM), R = 0.209, P = 0.001]. The relative abundance of 36 bacterial taxa was significantly higher, and another 24 kinds of bacteria were significantly lower in the RUTI group compared with the control group [LEfSe analysis, P < 0.05, linear discriminative analysis (LDA) score > 3], suggesting that Ralstonia, Prevotella, Dialister, and Corynebacterium may play an important role in RUTI.ConclusionThe urinary microbiota of women with clinically diagnosed RUTI were significantly different from age-matched asymptomatic controls.

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Section: Discussionmentioning

confidence: 97%

Differential Urinary Microbiota Composition Between Women With and Without Recurrent Urinary Tract Infection

Huang

Zheng

et al. 2022

Front. Microbiol.

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“…подходы к антибиотикотерапии таких осложнений при трансуретральной хирургии, как и в целом при урологических вмешательствах, находятся в состоянии открытой дискуссии, причиной чему является не только стремительная эволюция оперативных методик и аппаратного оснащения, но и качественное изменение требований к медикации в периоперационном периоде, наряду с угрожающим темпом распространения антибиотикорезистентности микроорганизмов, имеющей региональные особенности. Безусловно, невозможно представить оценку эффективности мероприятий по ликвидации инфекционного процесса без анализа вышеупомянутых подходов к его идентификации, что, по мнению некоторых авторов, может приводить либо к существенно менее управляемому течению осложнений, а значит и избыточной антимикробной терапии с усугублением устойчивости патогенов, либо вовсе к генерализации субклинической инфекции разной степени тяжести с развитием отдалённых осложнений в скомпроментированных локусах [5,7,8,29,30]. Дальнейший анализ отчётов о лечении бактериальных осложнений несёт в себе заложенный фактор неоднородности подходов в отношении их определения, а также сроков и режимов терапии.…”

Section: факторы риска послеоперационных инфекционных осложненийunclassified

Risk factors, antibiotic prophylaxis, and treatment of urinary tract infection in transurethral surgery for benign prostatic hyperplasia

2022

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Discussion of risk factors for infectious complications in the general population of patients undergoing transurethral resection of the prostate, antibiotic prophylaxis and therapy has been going on for decades. Up to date, the problem of the manifestation of bacteriuria in clinical infectious processes at various periods, as well as the factors of the introduction of bacterial agents with their further subclinical circulation in the urinary and reproductive systems, seems to be little discussed. The insufficient knowledge of these issues is confirmed by a small number of studies covering mainly electrosurgical mono and bipolar technologies as the most common and studied over a long period of their existence. This review raises the problem of insufficient knowledge of the clinical structure of infections during transurethral surgery, assessment of their risk factors, antibiotic prophylaxis regimens and therapy.

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“…Some societies recommend lowering this cut-off value in symptomatic uncomplicated infections with only one identified uropathogen to 10 2 CFU/ml [ 41 , 42 , 46 , 55 , 62 ], as this low count of CFU suggests significant bacteria in these patients. It has even been proposed that for a more accurate diagnosis, each uropathogen should have its own unique threshold [ 35 , 50 ] due to the fact that even when a low CFU count is found, it might have a role in maintaining persistent inflammation in the bladder, especially for those bacteria having specific virulence factors [ 63 ].…”

Section: Diagnosis Of Ruti and Abmentioning

confidence: 99%

Recurrent Urinary Tract Infections and Asymptomatic Bacteriuria in Adults

Hernández-Hernández

Padilla‐Fernández

Ortega-González

et al. 2021

Curr Bladder Dysfunct Rep

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Does Escherichia coli have pathogenic potential at a low level of bacteriuria in recurrent, uncomplicated urinary tract infection?

Cited by 10 publications

References 24 publications

Differential Urinary Microbiota Composition Between Women With and Without Recurrent Urinary Tract Infection

Differential Urinary Microbiota Composition Between Women With and Without Recurrent Urinary Tract Infection

Risk factors, antibiotic prophylaxis, and treatment of urinary tract infection in transurethral surgery for benign prostatic hyperplasia

Recurrent Urinary Tract Infections and Asymptomatic Bacteriuria in Adults

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