Does dimethyl sulfoxide increase protein immunomarking efficiency for dispersal and predation studies?

Williams, Louis O.; Hagler, James R.; Tonkel, Kirk C.

doi:10.1111/eea.12100

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Further research is needed to determine whether various biological solvents [e.g. dimethyl sulphoxide (DMSO), Sylgard â , Dow Chemical Co., Midland, MI] could be added to the protein mixtures to increase protein mark acquisition and retention on various insect species (Jones et al 2011;Williams et al 2013).…”

Section: Markmentioning

confidence: 99%

Development of a standardized protein immunomarking protocol for insect mark–capture dispersal research

Hagler

Naranjo

Machtley

et al. 2014

J Applied Entomology

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A field study was conducted to test the marking efficiency of broadcast spray applications of protein marks on stationary (represented by cadavers) and free‐roaming lady beetles Hippodamia convergens Guérin‐Méneville that were strategically placed in blooming alfalfa plots. The marks tested included three different concentrations of egg albumin from chicken egg white, casein from bovine milk and trypsin inhibitor from soy milk. The cadaver and free‐roaming beetle treatments served to measure the acquisition and retention of each protein treatment regime by direct contact with the spray solution and by residual contact with protein‐marked residue on alfalfa, respectively. In addition, the vertical distribution of marking efficacy was determined by sampling alfalfa plant tissue and beetle cadavers that were located on the upper and lower portion of the plant canopy. The data indicated that the backpack spray apparatus was very effective at uniformly administering the various protein marks, regardless of the concentration, throughout the entire plant canopy. Also, the free‐roaming beetles readily self‐marked by contact exposure to protein‐treated plants. We also identified concentrations of each protein type that will mark about 90% of the resident beetle population. Moreover, if a mark–capture‐type study only requires two unique protein marks, we determined that concentrations of 25% for egg white and 100% for bovine milk could be used to mark 98% of the population. Our results provide a significant step towards standardizing protein immunomarking protocols for insect mark–capture dispersal research. In addition, we identify several areas of research that are needed to further standardize the protein mark–capture procedure.

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Section: Markmentioning

confidence: 99%

Development of a standardized protein immunomarking protocol for insect mark–capture dispersal research

Hagler

Naranjo

Machtley

et al. 2014

J Applied Entomology

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“…Hagler & Jones () showed that Hippodamia convergens Guérin‐Méneville and Lygus hesperus Knight obtained an egg albumin mark after only 5 min of contact exposure to protein‐treated cotton leaf tissue; however, acquisition of the protein by Trichoplusia ni (Hübner) took between 20 and 240 min. Various additives incorporated into protein solutions may also influence protein detection; however, their effects remain uncertain (Jones et al., ; Williams et al., ). The water softener, EDTA, when added to protein solutions resulted in a positive effect (Jones et al., ) or no effect (Boina et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, protein persistence on pubescent (apple leaves used in Jones et al., ) vs. glossy (citrus leaves used in Boina et al., ) leaf surfaces has not been tested. The biological solvent, dimethyl sulfoxide, when added to the protein solutions enhanced rabbit and chicken IgG protein retention for marking a beetle and its predator, but may have had a deterrent effect on beetles (Williams et al., ). Future studies are needed to test the effect of these factors on protein mark persistence and insect behavior in the environment.…”

Section: Discussionmentioning

confidence: 99%

Evaluating Drosophila suzukii immunomarking for mark‐capture research

Klick

Lee

Hagler

et al. 2014

Entomologia Exp Applicata

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Drosophila suzukii Matsumura (Diptera: Drosophilidae) utilizes ‘Himalaya’ blackberry, Rubus armeniacus Focke (Rosaceae), as a host and may invade berry and stone fruit crops from field margins containing this invasive weed. Laboratory and semi‐field studies were conducted to determine (1) the persistence of protein marks including 10% chicken egg whites (egg albumin protein), 20% bovine milk (milk casein protein), and 20% soy milk (soy trypsin inhibitor protein) on topically sprayed D. suzukii, (2) protein retention on blackberry leaves, and (3) D. suzukii acquisition of protein after exposure to marked blackberry leaves for up to 14 days after application. All flies and leaves were assayed for the presence of the protein marks using protein‐specific enzyme‐linked immunosorbent assays. Egg albumin, milk casein, and soy trypsin proteins persisted on 94, 49, and 25% of the topically marked D. suzukii, respectively, throughout the 14‐day study period. Egg albumin was retained on 100% of treated leaves for 14 days, regardless of environmental conditions. At least 50% of flies exposed residually to egg albumin‐treated leaves were marked for 3 days, regardless of exposure time and environmental conditions. However, increasing fly exposure time to treated leaves in April and June appeared to improve protein mark acquisition. Acquisition of protein by flies from treated leaves for milk casein was inconsistent, and poor for soy trypsin, despite detectable levels on treated leaves. Egg albumin had the longest and most consistent persistence on flies, leaves, and flies exposed to leaves in laboratory and semi‐field studies, under a variety of environmental conditions and exposure times.

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“…bras., Brasília, v.50, n.5, p.399-406, maio 2015 DOI: 10.1590/S0100-204X2015000500007 immunomarking -enzyme-linked immunosorbent assay (Elisa) -has been developed for marking insects. Its benefits include being of low cost, simple, nontoxic, rapid, sensitive, applicable on a large scale, and capable of marking small insects (Jasrotia & Ben-Yakir, 2006;Williams et al, 2013). Therefore, this method may be used over large areas, to study the dispersion of insects, such as thrips.…”

Section: Introductionmentioning

confidence: 99%

Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato

Fernandes

2015

Pesq. agropec. bras.

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-The objective of this work was to evaluate the spatial distribution of thrips in different crops, and the correlation between meterological parameters and the flight movements of this pest, using immunomarking. The experiment was conducted in cultivated areas, with tomato (Solanum lycopersicum), potato (Solanum tuberosum), and onion (Allium cepa); and non-cultivated areas, with weedy plants. The areas with tomato (100 days), potato (20 days), and weeds were sprayed with casein, albumin, and soy milk, respectively, to mark adult thrips; however, the areas with onion (50 days) and tomato (10 days) were not sprayed. Thrips were captured with georeferenced blue sticky traps, transferred into tubes, and identified by treatment area with the Elisa test. The dependence between the samples and the capture distance was determined using geostatistics. Meteorlogical parameters were correlated with thrips density in each area. The three protein types used for immunomarking were detected in different proportions in the thrips. There was a correlation between casein-marked thrips and wind speed. The thrips flew a maximum distance of 3.5 km and dispersed from the older (tomato) to the younger crops (potato). The immunomarking method is efficient to mark large quantities of thrips.Index terms: Alliaceae, dispersal pattern, Elisa, integrated pest management, Solanaceae, Thysanoptera. Movimento de voo e distribuição espacial de tripes imunomarcados em cebola, batata e tomateResumo -O objetivo deste trabalho foi avaliar a distribuição espacial de tripes em diferentes cultivos, e a correlação entre os parâmetros meteorológicos e o movimento de voo dessa praga, com uso de imunomarcação. O experimento foi conduzido em área cultivada com tomate (Solanum lycopersicum), batata (Solanum tuberosum) e cebola (Allium cepa); e não cultivada, com plantas daninhas. As áreas com tomate (100 dias), batata (20 dias) e plantas daninhas foram pulverizadas com caseína, albumina e leite de soja, respectivamente, para marcar os tripes adultos; no entanto, as áreas com cebola (50 dias) e tomate (10 dias) não foram pulverizadas. Os tripes foram capturados com armadilha adesiva de cor azul georeferenciada, acondicionados em tubos e identificados, por área de tratamento, com o teste de Elisa. Determinou-se a dependência entre as amostras e a distância de captura com uso de geoestatística. Os parâmetros meteorológicos foram correlacionados com a densidade de tripes em cada área. Os três tipos de proteína usados na imunomarcação foram detectados, em diferentes proporções, nos tripes. Houve correlação entre os tripes marcados com caseína e a velocidade do vento. Os tripes voaram uma distância máxima de 3,5 km e dispersaram dos cultivos mais velhos (tomate) para os mais jovens (batata). O método de imunomarcação é eficiente para marcar grandes quantidades de tripes.Termos para indexação: Alliaceae, padrão de dispersão, Elisa, manejo integrado de pragas, Solanaceae, Thysanoptera.

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Does dimethyl sulfoxide increase protein immunomarking efficiency for dispersal and predation studies?

Cited by 6 publications

References 42 publications

Development of a standardized protein immunomarking protocol for insect mark–capture dispersal research

Development of a standardized protein immunomarking protocol for insect mark–capture dispersal research

Evaluating Drosophila suzukii immunomarking for mark‐capture research

Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato

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