Does crustacean ethoxyresorufin <i>O</i>‐deethylase activity vary during the molting cycle?

Hotard, Kate; Zou, Enmin

doi:10.1002/etc.2325

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…These stages were selected for study as they appear at the same time in synchronised cohorts of developing parasites and have approximately the same size and are well defined physiologically, whereas the large adult females undergo significant post-moulting growth and cycles of egg production and vitellogenesis [81], making this stage heterogeneous. While the moulting cycle can strongly affect CYP expression in crustaceans [82, 83], as can be expected for CYPs involved in ecdysteroid biosynthesis and metabolism, L. salmonis halloween genes and CYP18P1 were not found to be differentially expressed between preadult-II females and adult males in this study. Sex-biased transcript expression of CYPs in L. salmonis has previously been described from a microarray study, which included 12 CYPs, of which six showed sex-biased transcription [84].…”

Section: Discussionmentioning

confidence: 48%

Genome-wide survey of cytochrome P450 genes in the salmon louse Lepeophtheirus salmonis (Krøyer, 1837)

et al. 2019

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BackgroundThe salmon louse (Lepeophtheirus salmonis) infests farmed and wild salmonid fishes, causing considerable economic damage to the salmon farming industry. Infestations of farmed salmon are controlled using a combination of non-medicinal approaches and veterinary drug treatments. While L. salmonis has developed resistance to most available salmon delousing agents, relatively little is known about the molecular mechanisms involved. Members of the cytochrome P450 (CYP) superfamily are typically monooxygenases, some of which are involved in the biosynthesis and metabolism of endogenous compounds, while others have central roles in the detoxification of xenobiotics. In terrestrial arthropods, insecticide resistance can be based on the enhanced expression of CYPs. The reported research aimed to characterise the CYP superfamily in L. salmonis and assess its potential roles in drug resistance.MethodsLepeophtheirus salmonis CYPs were identified by homology searches of the genome and transcriptome of the parasite. CYP transcript abundance in drug susceptible and multi-resistant L. salmonis was assessed by quantitative reverse transcription PCR, taking into account both constitutive expression and expression in parasites exposed to sublethal levels of salmon delousing agents, ecdysteroids and environmental chemicals.ResultsThe above strategy led to the identification of 25 CYP genes/pseudogenes in L. salmonis, making its CYP superfamily the most compact characterised for any arthropod to date. Lepeophtheirus salmonis possesses homologues of a number of arthropod CYP genes with roles in ecdysteroid metabolism, such as the fruit fly genes disembodied, shadow, shade, spook and Cyp18a1. CYP transcript expression did not differ between one drug susceptible and one multi-resistant strain of L. salmonis. Exposure of L. salmonis to emamectin benzoate or deltamethrin caused the transcriptional upregulation of certain CYPs. In contrast, neither ecdysteroid nor benzo[a]pyrene exposure affected CYP transcription significantly.ConclusionsThe parasite L. salmonis is demonstrated to possess the most compact CYP superfamily characterised for any arthropod to date. The complement of CYP genes in L. salmonis includes conserved CYP genes involved in ecdysteroid biosynthesis and metabolism, as well as drug-inducible CYP genes. The present study does not provide evidence for a role of CYP genes in the decreased susceptibility of the multiresistant parasite strain studied.

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Section: Discussionmentioning

confidence: 48%

Genome-wide survey of cytochrome P450 genes in the salmon louse Lepeophtheirus salmonis (Krøyer, 1837)

et al. 2019

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Invisible endocrine disruption and its mechanisms: A current review

Zou

2020

General and Comparative Endocrinology

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Effects of nonylphenol on expression levels of GnRH-like polypeptide in Cipangopaludina chinensis

Wang¹,

Cai²,

Liu³

et al. 2015

Proceedings of the 2015 International Symposium on Material, Energy and Environment Engineering

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Abstract. Nonylphenol plays the role of estrogen in influencing male organisms in water much higher than the female biological. It is typical endocrine disruptors and does harm to biological reproductive system. Gonadotropin-releasing hormone (GnRH) is a sex hormone-containing nerve decape-ptide.The main physiological role is associated with pituitary gonadotropin secretion cells specifically bind to receptors to promote cell gonadotropin secretion. In this experiment, Cipangopaludina chinensis was as experimental subjects to detect and observe the trend of nonylphenol on ovary, brain sections, and the visceral ganglion and foot ganglion GnRH-like Peptide expression in C. chinensis which lined in bottom sediments. Then, it was discussed on the toxic effects of nonylphenol on C. chinensis in bottom sediments and provides some reasonable suggestions to address the environmental impact of hormones on aquatic organisms.

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Does crustacean ethoxyresorufin O‐deethylase activity vary during the molting cycle?

Cited by 3 publications

References 23 publications

Genome-wide survey of cytochrome P450 genes in the salmon louse Lepeophtheirus salmonis (Krøyer, 1837)

Genome-wide survey of cytochrome P450 genes in the salmon louse Lepeophtheirus salmonis (Krøyer, 1837)

Invisible endocrine disruption and its mechanisms: A current review

Effects of nonylphenol on expression levels of GnRH-like polypeptide in Cipangopaludina chinensis

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