Does Cord Blood Transplantation Result in Lower Graft-Versus-Host Disease?

Madrigal, J. Alejandro; Cohen, S. B. A.; Gluckman, Éliane; Charron, Dominique

doi:10.1016/s0198-8859(97)00125-0

Cited by 74 publications

(39 citation statements)

References 12 publications

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“…7,42,43 We recently demonstrated that cord blood transplant recipients, in particular those who did not receive total body irradiation in the conditioning regimen and did not experience GvHD, may harbor several circulating Tlymphocyte clones of recipient origin, specific for widespread pathogens, such as human cytomegalovirus and Candida albicans. 29 The early post-transplantation occurrence of episodes of infection/reactivation, together with the naïve state of cord blood T lymphocytes adoptively transferred with the graft, could also have favored the establishment of mixed chimerism.…”

Section: Discussionmentioning

confidence: 99%

Donor/recipient mixed chimerism does not predict graft failure in children with -thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling

et al. 2008

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Section: Discussionmentioning

confidence: 99%

Donor/recipient mixed chimerism does not predict graft failure in children with -thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling

et al. 2008

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“…It is widely accepted that activation of mature donor T lymphocytes, after exposure to the antigens of the recipient, is the main event involved in the onset of acute GVHD. 42,43 For this reason, most of the studies on the immaturity of the CB immune system are focused on the evaluation of the relative numbers of activated (defined as CD45RO + ) with respect to naive or unprimed (defined as CD45RA + ) T cells present in these specimens. We confirm the previously reported differences 44 between CB and adult PB in the number of T cells expressing CD45RA or CD45RO.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the T cell receptor repertoire of neonatal T cells by RT-PCR and single strand conformation polymorphism analysis

Alfani

Migliaccio

Sanchez

et al. 2000

Bone Marrow Transplant

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Summary:We have analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) the individual non-germ line configurations of the T cell receptor (TCR) V␤ chains expressed by T cells from eight individual cord blood specimens. cDNA from each cord blood was amplified using a common primer coupled with a primer specific for each of 22 variable elements of the V␤ chain family and the amplified fragments were separated under high resolution conditions. With cDNA from adult blood (as a control), all of the TCR chains were amplified as a smear consistent with the extensive polyclonality of adult T cells. In contrast, a heterogeneous pattern of amplification was observed with cDNAs from cord blood: only 26.7 ± 21.9% of the 22 V␤ chains analyzed were amplified as a smear. The majority of them were amplified as a discrete number of bands (up to 10) (in 68.2 ± 18.7% of samples) and some of them as a single fragment (4.0 ± 7.8%). Only one of the eight samples analyzed expressed the majority (72.7%) of its V␤ chains as a smear, consistent with an adult-like TCR repertoire. In conclusion, cord blood expressed, on average, a less complex TCR repertoire than adult blood. Bone Marrow Transplantation (2000) 26, 83-89. Keywords: cord blood; T cell receptor; single strand conformation polymorphism; cord blood transplantation; graft-versus-host disease T lymphocytes are cells produced in the thymus that, once activated, are responsible for the humoral and cellular immune responses. The process of T cell activation is mediated by the T cell receptor (TCR), a membrane-bound heterodimer composed of an ␣ and a ␤ chain, that recognizes antigens once coupled with the major histocompatibility complex expressed by antigen presenting cells (APC). During the process of T cell differentiation, the ␤ chains undergo rearrangements through somatic recombination among multiple coding segments, namely the variable (V), diversity (D), joining (J) and constant (C) genes. 1,2 At present, more than 65 different V␤ genes, a quarter of which are represented by pseudogenes, have been identified. [3][4][5] The V(D)J junctional diversity is further increased by other molecular mechanisms which include imprecise joining of V(D)J recombination, 6 N-region diversification (random addition of nucleotides by terminal deoxy-ribonucleotidyl transferase) 7,8 and insertions of palindromic nucleotides 9 at specific points of the VD, DJ and VJ junctions. As a result of allele exclusion, 10 each T cell clone expresses one and only one specifically rearranged TCR V␤ chain. Therefore, the number of TCR V␤ chain rearrangements expressed by a given population correlates with its T cell clonality.Cord blood (CB) has been proven to be a good source of stem/progenitor cells for related and unrelated transplants. 11,12 Successful engraftment with low graft-versushost disease (GVHD) has been observed even in the case of unrelated poorly matched transplants. 13,14 The reduced GVHD severity observed in allogeneic CB transplantation is explained by the low immunologic react...

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“…Compared to peripheral blood, cord blood contains a higher proportion of primitive HSC (CD34+HLA-DR-) and of immature and non-functional T cells (Cairo & Wagner 1997, Madrigal et al 1997, Dimitriou et al 1998). These features suggest that cord blood cells may be more efficient and cause less GVHD than other sources of HSCT.…”

Section: Clinical Applications Of Flow Cytometry To Stem Cell Transplmentioning

confidence: 99%

Applications of flow cytometry to hematopoietic stem cell transplantation

Voltarelli

2000

Mem. Inst. Oswaldo Cruz

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Does Cord Blood Transplantation Result in Lower Graft-Versus-Host Disease?

Cited by 74 publications

References 12 publications

Donor/recipient mixed chimerism does not predict graft failure in children with -thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling

Donor/recipient mixed chimerism does not predict graft failure in children with -thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling

Characterization of the T cell receptor repertoire of neonatal T cells by RT-PCR and single strand conformation polymorphism analysis

Applications of flow cytometry to hematopoietic stem cell transplantation

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