Does copper stress lead to spindle misposition-dependent cell cycle arrest?

Abstract

ABSTRACT. Because of its specific electrochemical properties, copper is an essential heavy metal for living organisms. As with other heavy metals, high levels can provoke damage. We examined gene expression under copper stress in wild-type fission yeast (Schizosaccharomyces pombe) through differential display. After the EC 50 concentration of CuSO 4 was determined as 50 µM, total RNA was isolated from cells treated or not with copper. The expression level of SPCC1682.13, ppk1, SPBC2F12.05c, and adg2 genes increased significantly under copper stress. Considering the functions of these genes are related to the cell cycle, cell division and chromosome dynamics, we hypothesize that retardation of the cell cycle under copper stress is relevant to the events that depend on the functions of these genes.

Copper transport and regulation in Schizosaccharomyces pombe

“…On the other hand, copper can engage in detrimental chemical reactions or compete with other metal ions at enzyme-active sites, due to its ability to lose or gain a single electron [2]. It has also been reported that excess copper may cause cell-cycle misregulation [3]. It is therefore critical that cells use tightly regulated homoeostatic mechanisms to acquire sufficient amounts of copper, while at the same time preventing its accumulation to cytotoxic levels.…”