Does chlorophyllidea reduce reliability of chlorophylla measurements in marine coastal sediments?

Plante-Cuny, Marie-Reine; Barranguet, Christiane; Bonin, D. J.; Grenz, Christian

doi:10.1007/bf00877256

Cited by 27 publications

(21 citation statements)

References 34 publications

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“…In the stations which presented trations by up to 90%. However, by means of a previ- ous comparative study of pigment extraction from wet and freeze-dried sediments, we concluded that results obtained from wet samples were highly variable and non-reproducible (Plante-Cuny et al 1993). Deepfreezing and subsequent thawing before extraction appears to be much more harmful than using freezedried samples.…”

Section: Introductionmentioning

confidence: 99%

“…The HPLC technique, being essentially a separative method, makes it possible to isolate native chl a which can be used as a reliable index of this plant 'living mass' (biomass) of microbenthic algae (Riaux-Gobin et al 1987, Barlow et al 1990, Plante-Cuny et al 1993, Pinckney et al 1994). …”

Section: Introductionmentioning

confidence: 99%

“…The latter method furthermore allows long-term conservation. Some other problems, also discussed by Plante-Cuny et al (1993), may occur during extraction or when using the HPLC technique, especially through chlorophyllase action (Jeffrey & Hallegraeff 1987). Water content was determined as the percentage of water in relation to total fresh weight.…”

Section: Introductionmentioning

confidence: 99%

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Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal) using pigment analysis by HPLC

Brotas¹,

Plante-Cuny²

1998

Mar. Ecol. Prog. Ser.

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ABSTRACT. Measurements of natural estuarine microphytobenthos communities were carried out in samples of intertidal sedlrnents of the Tagus Estuary (Portugal) by means of pigment analysis by HPLC in order to identify microphytobenthos composition. The relative abundance of taxa was determined by microscopic observations. During spring 16 sites were sampled and 3 of them, differing in sediment grain size and tidal level, were, \ isited fortnightly for 1 yr. At all sites throughout the year, diatoms were the dominant algal group, evidenced by the. relatively high concentrations of chlorophylls cI+c2, fucoxanthin, neofucoxanthin, diadinoxanthin, diatoxanthin and 0-carotene. Chl b and diatoxanthin were related with observed euglenophyte populations, zeaxanthin with cyanobacteria, lutein with macrophyte debris and alloxanthin with falling phytoplanktonic cells of cryptophytes. The spatial study allowed a distinction between sites regarding the presence of macrophyte debris, euglenophytes or only diatolns. The temporal survey showed the seasonal trends of diatoms and cyanobacteria and the main periods of plant debris deposition. Pheophytin a and chlorophyllide a concentrations were quite low. Degraded chloropigments were constituted mainly of pheophorbides a, 9 of which were quantified. The most abundant among them indicated intensive grazing by ~nacrozoobenthos at the muddy stations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal) using pigment analysis by HPLC

Brotas¹,

Plante-Cuny²

1998

Mar. Ecol. Prog. Ser.

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“…We have indeed detected many forms of phaeopigment a, which is consistent with findings of other studies concerning sediment (Brown et al 1981, Riaux-Gobin et al 1987, Klein et Riaux-Gobin 1991, Yacobi et al 1991. The 4 phaeophorbides, which represented about 7 5 % of total phaeopigments, are markers for macrobenthic deposit-feeding processes (Bianchi et al 1988(Bianchi et al , 1993 and more generally for metazoan grazing activity (Brown et al 1981, Welschmeyer & Lorenzen 1985, Abele-Oeschger et al 1992 Based on their order of elution and retention times, the 2 major phaeophorbides a-like:, and a-like, (accounting for about 50 % of total phaeophorbides) correspond very likely to the phaeophorbides a-like in Hawkins et al (1986) study and to phaeophorbides a, and a3 reported by Plante-Cuny et al (1993). In these studies, the 2 degraded pigments are reported to result from chl a digestion by mussels.…”

Section: Qualitative Analysismentioning

confidence: 99%

“…High-performance liquid chromatography (HPLC) analysis is now commonly used in ecological studies concerning the sediment, which have largely focused on the problem of method intercalibration (usually between HPLC and the nonseparative spectrophotometric and fluorometric methods) for chloropigment measurements (Brown et al 1981, Daemen 1986, Barlow et a1 1990, Neveux et al 1990, Plante-Cuny et al 1993. Apart from these methodological works, investigations on sediment pigment measurements by HPLC h a r e also been conducted to estimate algal pigment diversity in coastal sediments (Riaux-Gobin et al 1987, Klein & RiauxGobin 1991, to trace the origin and the fate of plant material (Bianchi & Findlay 19W.…”

Section: Introductionmentioning

confidence: 99%

Monthly HPLC measurements of pigment concentration from an intertidal muddy sediment of Marennes-Oléron Bay, France

Gall¹,

Blanchard²

1995

Mar. Ecol. Prog. Ser.

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Chloropigments and carotenoids were measured by HPLC in an intertidal muddy sediment of Marennes-Oleron Bay (France). Concentrations were determined as a function of s-ent depth at low tide. The analyses were carried out at monthly intervals over l yr. Pigment analindicated that the microphytobenthic community was dominated by diatoms throughout the sampling year. Chlorophyll b was not encountered at any time or depth, indicating that no input from macmphytic detritus had occurred at the sampling site. There was a large pool of phaeopigments a. d whlch phaeophorbides were the major forms (75%). A microphytobenthic bloom occurred between March and June during which phaeopigments significantly increased with a high proportion of phaeophorb~d e s likely due to intense grazing activ~ty of benthic invertebrates. P~gment concentrat~ons were still high at 5 cm depth and changes occurred simultaneously in the aphotic (<5 mm sediment depLh] and photic (>5 mm sediment depth) layers of the sediment, reflecting an impact of bioturbation and physical mixing (resuspension/redeposition) of the upper sediment layer. There was a decrease of the pigment content of the sediment between June and July. It was attributed to grazing and to a resuspension event. Overall, the pigment analysis of this intertidal sediment indicates a predominance of t h e diatom community, a close coupling between the dynamics of microphytobenthos biomass and the grazing activity of benthic invertebrates, and the influence of sediment mixing.

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Aquatic sediments

Sylvester¹,

Garton²,

Autenrieth³

1994

Water Environment Research

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Does chlorophyllidea reduce reliability of chlorophylla measurements in marine coastal sediments?

Cited by 27 publications

References 34 publications

Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal) using pigment analysis by HPLC

Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal) using pigment analysis by HPLC

Monthly HPLC measurements of pigment concentration from an intertidal muddy sediment of Marennes-Oléron Bay, France

Aquatic sediments

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