Does CCR5 gene-D32 deletion protect C.trachomatis infected Indian women from tubal pathology?

Abstract

Deletion in chemokine receptor CCR5 gene is reported to prevent development of tubal pathology among Dutch Caucasian C. trachomatis infected women. Hence, a pilot study was undertaken, to evaluate the involvement of CCR5 gene in tubal pathology among Indian women with or without Chlamydia infection. Three hundred women with or without Chlamydia infection and with different reproductive manifestations were screened for CCR5 gene using a standardized PCR. Only 6 C. trachomatis infected women without tubal block had heterozygous deletion for this gene. The rest had wild type CCR5 gene, that includes fertile women infected and non infected; infertile women infected and non infected with and without tubal block as well as infected women with history of recurrent spontaneous abortions. The study indicates no role of heterozygous CCR5 D32 gene deletion in prevention of tubal pathology in Chlamydia infected Indian women. Only 2% heterozygous deletion in CCR5 gene is observed in the studied population.

Chemokine receptors CCR5 and CCR2 genes in HIV positive, HIV exposed seronegative and in HIV unexposed individuals: A study from Mumbai

“…The CCR5 32 deletion was genotyped by detecting size differences in the amplicons on 3% agarose gel; wild type (WT) CCR5 gene with 189 bp product, homozygote CCR5 mutant gene with 157 bp, and the heterozygote CCR5 gene with both 189 and 157 bp products. [3] No homozygous or heterozygous deletion was observed in CCR5 gene; all were with wild type gene [Figure 2a]. Our observation on complete absence of CCR5∆32 deletion is consistent with a report from a north Indian population.…”

“…Our observation on complete absence of CCR5∆32 deletion is consistent with a report from a north Indian population. [4] Although a previous study has shown a 2% heterozygous CCR5∆32 deletion in 300 studied women, these 6 women belonged to the Muslim community, [3] as was reported among the Muslims of northern India. In the present study none of the subjects were Muslim.…”

CCR5 and CCR5Δ32 in bacterial and parasitic infections: Thinking chemokine receptors outside the HIV box

“…However, the sample size used in this specific analysis was limited ( n = 41 in total), and this result must be considered with prudence. Moreover, the CCR5∆32 was not a protective factor of tubal pathology development in C. trachomatis ‐infected women in a subsequent study that evaluated 174 women with reproductive problems (99 positive for C. trachomatis ) and 126 fertile women (42 positive for C. trachomatis ), both groups from India (Mania‐Pramanik, Kerkar, Vallabhadas, Mehta, & Salvi, 2011).…”