Does biopolymers composition in seeds contribute to the flax resistance against the <i>Fusarium</i> infection?

Zeitoun, A.; Preisner, Marta; Kulma, Anna; Dymińska, Lucyna; Hanuza, J.; Starzycki, M.; Szopa, Jan

doi:10.1002/btpr.1965

Cited by 8 publications

(6 citation statements)

References 54 publications

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“…The esterification of these compounds in the cell walls forms a physical barrier against fungal penetration and enzymes responsible for damaging cell walls. Thus the increased level of lignin precursors may be implicated in the resistance responses of vanilla to Forv infection ( Hammerschmidt and Kuc, 1982 ; Cvikrová et al, 1993 ; El Modafar and El Boustani, 2001 ; de Ascensao and Dubery, 2003 ; Zeitoun et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Differential Responses of Vanilla Accessions to Root Rot and Colonization by Fusarium oxysporum f. sp. radicis-vanillae

et al. 2015

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Root and stem rot (RSR) disease caused by Fusarium oxysporum f. sp. radicis-vanillae (Forv) is the most damaging disease of vanilla (Vanilla planifolia and V. × tahitensis, Orchidaceae). Breeding programs aimed at developing resistant vanilla varieties are hampered by the scarcity of sources of resistance to RSR and insufficient knowledge about the histopathology of Forv. In this work we have (i) identified new genetic resources resistant to RSR including V. planifolia inbreds and vanilla relatives, (ii) thoroughly described the colonization pattern of Forv into selected vanilla accessions, confirming its necrotic non-vascular behavior in roots, and (iii) evidenced the key role played by hypodermis, and particularly lignin deposition onto hypodermal cell walls, for resistance to Forv in two highly resistant vanilla accessions. Two hundred and fifty-four vanilla accessions were evaluated in the field under natural conditions of infection and in controlled conditions using in vitro plants root-dip inoculated by the highly pathogenic isolate Fo072. For the 26 accessions evaluated in both conditions, a high correlation was observed between field evaluation and in vitro assay. The root infection process and plant response of one susceptible and two resistant accessions challenged with Fo072 were studied using wide field and multiphoton microscopy. In susceptible V. planifolia, hyphae penetrated directly into the rhizodermis in the hairy root region then invaded the cortex through the passage cells where it induced plasmolysis, but never reached the vascular region. In the case of the resistant accessions, the penetration was stopped at the hypodermal layer. Anatomical and histochemical observations coupled with spectral analysis of the hypodermis suggested the role of lignin deposition in the resistance to Forv. The thickness of lignin constitutively deposited onto outer cell walls of hypodermis was highly correlated with the level of resistance for 21 accessions tested. The accumulation of p-coumaric and sinapic acids, two phenolic precursors of lignin, was observed in the resistant plants inoculated with Fo072, but not in the susceptible one. Altogether, our analyses enlightened the mechanisms at work in RSR resistant genotypes and should enhance the development of novel breeding strategies aimed at improving the genetic control of RSR of vanilla.

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Section: Discussionmentioning

confidence: 99%

Differential Responses of Vanilla Accessions to Root Rot and Colonization by Fusarium oxysporum f. sp. radicis-vanillae

et al. 2015

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“…The latter group also includes sections Linum (Schmidt et al 2010). The content of lignans in flaxseed significantly correlates with antioxidant activity (Kasote 2013;Zeitoun et al 2014;Kyselka et al 2017). The major flax lignan compounds, secoisolariciresinol (SECO), and its diglucoside (SDG) prove their antioxidant activity as well as antiviral, antibacterial, and antifungal properties (Kasote 2013;Zeitoun et al 2014;Kyselka et al 2017;Wu et al 2021).…”

Section: Lignans In Flaxmentioning

confidence: 99%

“…The content of lignans in flaxseed significantly correlates with antioxidant activity (Kasote 2013;Zeitoun et al 2014;Kyselka et al 2017). The major flax lignan compounds, secoisolariciresinol (SECO), and its diglucoside (SDG) prove their antioxidant activity as well as antiviral, antibacterial, and antifungal properties (Kasote 2013;Zeitoun et al 2014;Kyselka et al 2017;Wu et al 2021). Some types of lignans as pinoresinol, isolariciresinol, matairesinol, hydroxymatairesinol, and lariciresinol were detected in a mucilage layer of linseeds (Kyselka et al 2017).…”

Section: Lignans In Flaxmentioning

confidence: 99%

Biological functions of lignans in plants

Ražná

Nôžková

Vargaová

et al. 2021

Agriculture (Pol'nohospodárstvo)

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Naturally occurring lignans are present in seeds, nuts, cereals, vegetables, and fruits. Lignans play various roles in plants and their multipurpose functions of different organisms including humans is considerable. They are involved in plant defence mechanisms through their antioxidant, antiviral, antibacterial, and antifungal properties. The lignans content may be affected by a variety of factors such as genotype, tissue type, geographic origin, local environmental conditions, nutrition, and plant maturity. Interactive relationships between individual factors are also considered. This review aimed to summarize the biological functions of lignans for plants and empasize the importance of these compounds for the added value of individual genotypes of plant food resources. Understanding the biological functions of lignans in plants can provide solutions to the ever-increasing requirements for the production of functional foods. Flaxseed is the richest source of lignans, and as such is considered the model species for lignans studies. Within our review, one paragraph is focused on the properties and biological functions of flax lignans.

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“…However, the molecular mechanisms underlying resistance to Fusarium wilt remain unclear, and the search for genes involved in the response to F. oxysporum is an area of active research. The involvement of pathogenesisrelated proteins in the response to F. oxysporum infection was demonstrated (Wrobel-Kwiatkowska et al, 2004;Wojtasik et al, 2014;Galindo-Gonzalez, Deyholos, 2016), and the roles of antioxidants, polyamines, and phenolic compounds in response to the pathogen were shown (Lorenc-Kukula et al, 2007Boba et al, 2011Boba et al, , 2016Zeitoun et al, 2014;Wojtasik et al, 2015). Moreover, F. oxysporum-infected flax plants show cell wall rearrangements (Wojtasik et al, 2015(Wojtasik et al, , 2016Boba et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The cinnamyl alcohol dehydrogenase gene family is involved in the response to Fusarium oxysporum in resistant and susceptible flax genotypes

et al. 2019

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Flax (Linum usitatissimum L.) is used for the production of textile, oils, pharmaceuticals, and composite materials. Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. lini, is a very harmful disease that reduces flax production. Flax cultivars that are resistant to Fusarium wilt have been developed, and the genes that are involved in the host response to F. oxysporum have been identified. However, the mechanisms underlying resistance to this pathogen remain unclear. In the present study, we used transcriptome sequencing data obtained from susceptible and resistant flax genotypes grown under control conditions or F. oxysporum infection. Approximately 250 million reads, generated with an Illumina NextSeq instrument, were analyzed. After filtering to exclude the F. oxysporum transcriptome, the remaining reads were mapped to the L. usitatissimum genome and quantified. Then, the expression levels of cinnamyl alcohol dehydrogenase (CAD) family genes, which are known to be involved in the response to F. oxysporum, were evaluated in resistant and susceptible flax genotypes. Expression alterations in response to the pathogen were detected for all 13 examined CAD genes. The most significant differences in expression between control and infected plants were observed for CAD1B, CAD4A, CAD5A, and CAD5B, with strong upregulation of CAD1B, CAD5A, and CAD5B and strong downregulation of CAD4A. When plants were grown under the same conditions, the expression levels were similar in all studied flax genotypes for most CAD genes, and statistically significant differences in expression between resistant and susceptible genotypes were only observed for CAD1A. Our study indicates the strong involvement of CAD genes in flax response to F. oxysporum but brings no evidence of their role as resistance gene candidates. These findings contribute to the understanding of the mechanisms underlying the response of flax to F. oxysporum infection and the role of CAD genes in stress resistance.

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Does biopolymers composition in seeds contribute to the flax resistance against the Fusarium infection?

Cited by 8 publications

References 54 publications

Differential Responses of Vanilla Accessions to Root Rot and Colonization by Fusarium oxysporum f. sp. radicis-vanillae

Differential Responses of Vanilla Accessions to Root Rot and Colonization by Fusarium oxysporum f. sp. radicis-vanillae

Biological functions of lignans in plants

The cinnamyl alcohol dehydrogenase gene family is involved in the response to Fusarium oxysporum in resistant and susceptible flax genotypes

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