Does a different dose of gamma irradiation have the same effect on five different types of tendon allografts? — a biomechanical study

Hangody, György Márk; Szebényi, Gábor; Abonyi, Bence; Kiss, Rita M.; Hangody, László; Pap, Károly

doi:10.1007/s00264-016-3336-7

Cited by 16 publications

(25 citation statements)

References 25 publications

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“…The results confirm the findings of our previous research [11]. The aggregate results of the present and prior studies [11,12] show that there is a difference in irradiation sensitivity of the examined tendons, which has to be considered before application. The tendons types which were the most affected by the treatments were quadricep grafts.…”

Section: Discussionsupporting

confidence: 92%

“…The typical force-elongation curves of each type of tendon are presented in Figure 2. According to the results of a preliminary study [11], the freezing, the irradiation, and thawing were unified at all graft types. Irradiation was performed on frozen grafts, with the same method at all grafts.…”

Section: Resultsmentioning

confidence: 99%

“…A radio-protectant solution is used to avoid side effects and protect tissues. A lower dose of gamma radiation (10-15 kGy) is bactericidal, whereas higher doses (30-50 kGy) are antiviral [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Effect of Freezing and Gamma Irradiation on Different Types of Tendon Allografts by DIC Assisted Tensile Testing

et al. 2020

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This study aimed to evaluate the changes in the endurance properties of four types of tendons caused by freezing and gamma irradiation. Four types of grafts were harvested: quadriceps, semitendinosus + gracilis, tibialis anterior, and peroneus longus. These were put into three groups: Group A was the control group, tested without freezing or irradiation. Grafts in Group B were frozen and irradiated (target dose: 21 kGy), while grafts in Group C were again frozen and irradiated (target dose: 42 kGy). Maximum load, tensile modulus, tensile strength, strain at maximum force and fracture strain were calculated from the force-elongation graphs of cyclic load tests. The higher gamma irradiation dose (Group C) significantly decreased the strains at tensile strength of the quadriceps tendons (p = 0.0004–0.0237), compared to the other two groups. In the case of the quadricep tendons (p = 0.0151), there is a significant decrease in Young’s modulus after gamma irradiation with the dose of 42 kGy. According to the results of the study, the tibialis anterior and the peroneus longus are recommended in ACL reconstruction when gamma irradiation is required, while quadricep tendons, which are usually used for an autograph, are not suitable for allograft reconstruction after gamma irradiation from the viewpoint of mechanical properties.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

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Evaluation of the Effect of Freezing and Gamma Irradiation on Different Types of Tendon Allografts by DIC Assisted Tensile Testing

et al. 2020

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“…Finally, the study was performed using rabbit tendons instead of human tissues. The procurement of human tendons for such a study would have been possible from cadavers, who are usually aged, and potentially have degenerative tendon tears 53 . By using animal tendons, we were able to standardize the procedure, and to implant treated scaffolds in the same species.…”

Section: Discussionmentioning

confidence: 99%

Use of supercritical carbon dioxide technology for fabricating a tissue engineering scaffold for anterior cruciate ligament repair

Sherifi

Bachy

Laumonier³

et al. 2020

Sci Rep

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Tissue-engineered grafts may be useful in Anterior Cruciate Ligament (ACL) repair and provide a novel, alternative treatment to clinical complications of rupture, harvest site morbidity and biocompatibility associated with autografts, allografts and synthetic grafts. We successfully used supercritical carbon dioxide (Sc-co 2) technology for manufacturing a "smart" biomaterial scaffold, which retains the native protein conformation and tensile strength of the natural ACL but is decellularized for a decreased immunogenic response. We designed and fabricated a new scaffold exhibiting (1) high tensile strength and biomechanical properties comparable to those of the native tissue, (2) thermodynamically-stable extra-cellular matrix (ECM), (3) preserved collagen composition and crosslinking, (4) a decellularized material milieu with potential for future engineering applications and (5) proven feasibility and biocompatibility in an animal model of ligament reconstruction. Because of the "smart" material ECM, this scaffold may have the potential for providing a niche and for directing stem cell growth, differentiations and function pertinent to new tissue formation. Sc-CO 2-related technology is advanced and has the capability to provide scaffolds of high strength and durability, which sustain a lifetime of wear and tear under mechanical loading in vivo. The anterior cruciate ligament (ACL) is the most frequently injured ligament in the knee. More than 200,000 patients are diagnosed yearly with ACL disruptions 1,2 and approximately 120,000 ACL reconstructions are performed annually in the United States 3. Because it receives nourishment mainly from the surrounding synovial fluid, the ACL has poor natural healing ability and thus necessitates surgical reconstruction when ruptured 4. Primary surgical repair of ligaments has shown poor results in clinical practice and is not commonly used as a treatment option today 5. Since the reconstructed knees need to last the lifetime of the patient, the repaired ligament needs to possess reliable durability under the body's mechanical loading and active stress during daily activities. This requirement translates into the need for a biomechanically and biologically stable replacement that must withstand a lifetime of wear and tear. Historically, the medical field has explored various approaches in search of the perfect tissue substitute for the natural ACL. Autografts, which involve reconstruction using the patient's own tendons to replace the damaged ACL, have offered good strength and served as successful surgical substitutes in 85-90% of clinical cases 6. However, autograft harvesting causes significant donor site morbidity, including anterior knee pain, patellar tendinitis, and infra-patellar contracture, following the harvesting of the patellar tendon; hamstring weakness, and saphenous nerve injury after harvesting of the hamstring tendons 7. Synthetic materials have been extensively studied as replacements of the native ACL 8,9. These synthetic substitutes have generally exhibite...

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“…As an allograft, Achilles tendon has been used to reconstruct joints 12,13 and to repair rotator cuff 14 , patellar tendon 15 , and anterior cruciate ligament 16 , but only occasionally transplanted to replace an Achilles tendon. In a group of 14 cases, an Achilles allograft was transplanted to reconstruct a large Achilles defect (7.0 + 3 cm) 17 .…”

Section: Introductionmentioning

confidence: 99%

Orthotopic Transplantation of Achilles Tendon Allograft in Rats

et al. 2018

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The biology and function of orthotopic transplantation of Achilles tendon allograft are unknown. Particularly, the revitalization of Achilles allograft is a clinical concern. Achilles allografts were harvested from donor rats and stored at −80 °C. Subcutaneous adipose tissue was harvested from the would-be allograft recipient rats for isolation of mesenchymal stem cells (MSCs). MSCs were cultured with growth differentiation factor-5 (GDF-5) and applied onto Achilles allografts on the day of transplantation. After the native Achilles tendon was resected from the left hind limb of the rats, Achilles allograft, with or without autologous MSCs, was implanted and sutured with calf muscles proximally and calcaneus distally. Animal gait was recorded presurgery and postsurgery weekly. The animals were sacrificed at week 4, and the transplanted Achilles allografts were collected for biomechanical testing and histology. The operated limbs had altered gait. By week 4, the paw print intensity, stance time, and duty cycle (percentage of the stance phase in a step cycle) of the reconstructed limbs were mostly recovered to the baselines recorded before surgery. Maximum load of failure was not different between Achilles allografts, with or without MSCs, and the native tendons. The Achilles allograft supplemented with MSCs had higher cellularity than the Achilles allograft without MSCs. Deposition of fine collagen (type III) fibers was active in Achilles allograft, with or without MSCs, but it was more evenly distributed in the allografts that were incubated with MSCs. In conclusion, orthotopically transplanted Achilles allograft healed with host tissues, regained strength, and largely restored Achilles function in 4 wk in rats. It is therefore a viable option for the reconstruction of a large Achilles tendon defect. Supplementation of MSCs improved repopulation of Achilles allograft, but large animal models, with long-term follow up and cell tracking, may be required to fully appreciate the functional benefits of MSCs.

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Does a different dose of gamma irradiation have the same effect on five different types of tendon allografts? — a biomechanical study

Abstract: The vulnerability of gamma irradiation of TA was less than Achilles and quadriceps tendons.

Cited by 16 publications

References 25 publications

Evaluation of the Effect of Freezing and Gamma Irradiation on Different Types of Tendon Allografts by DIC Assisted Tensile Testing

Evaluation of the Effect of Freezing and Gamma Irradiation on Different Types of Tendon Allografts by DIC Assisted Tensile Testing

Use of supercritical carbon dioxide technology for fabricating a tissue engineering scaffold for anterior cruciate ligament repair

Orthotopic Transplantation of Achilles Tendon Allograft in Rats

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