Doença do armazenamento lisossomal induzida pelo consumo de Sida carpinifolia em bovinos do Rio Grande do Sul

Pedroso, Pedro Miguel Ocampos; Oliveira, Luiz Gustavo Schneider de; Cruz, Cláudio Estêvão Farias da; Soares, Mauro Pereira; Barreto, Laura; Driemeier, David

doi:10.1590/s0100-736x2010001000005

Cited by 11 publications

(20 citation statements)

References 29 publications

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“…There was also moderate proliferation of Bergmann astrocytes in the cerebellum, moderate vacuolization of thyroid follicular epithelial cells and proximal convoluted tubular cells, and mild vacuolization of hepatocytes. These were similar to what has already been described in natural and experimental poisoning in cattle (Furlan et al 2008, Pedroso et al 2010, sheep (Seitz et al 2005), goats (Driemeier et al 2000, Colodel et al 2002a) and ponies (Loretti et al 2003). Other authors have also described cytoplasmic vacuolization of acinar pancreatic epithelial cells (Driemeier et al 2000, Seitz et al 2005, Furlan et al 2008, Pedroso et al 2010.…”

Section: Discussionsupporting

confidence: 87%

“…Brain sections (cerebellum, pons, thalamus and hippocampus) were stained using lectin histochemistry (Goldstein & Hayes 1978) with commercial lectins (Vector Laboratories, Burlingame, CA) (Chart 1) through a previously protocol described by Pedroso et al 2010. The lectins were diluted to 5mg/ml in PBS (phosphate buffered saline), except for Concanavalia ensiformis lectin, which was diluted to 1mg/ml.…”

Section: Methodsmentioning

confidence: 99%

“…Sida carpinifolia is an American native plant that occurs in almost all of the national territory (Kissmann & Groth 2000, Lorenzi 2000. In the state of Rio Grande do Sul, this plant occurs mainly in humid and shady areas, where it has already been diagnosed as a cause of poisoning in goats (Driemeier et al 2000), ponies (Loretti et al 2003), sheep (Seitz et al 2005), cattle (Furlan et al 2009, Pedroso et al 2010, and deer (Dama dama) (Pedroso et al 2009). The poisoning is associated with lysosomal storage of oligosaccharides caused by the indolizidine alkaloid swainsonine (Colodel et al 2002b), which triggers the inhibition of lysosomal α-mannosidase and α-mannosidase II from the Golgi complex (Agamanolis 1995, Moremen 2002, thus affecting neurons (Molyneux & James 1982, Stegelmeier et al 1995.…”

Section: Introductionmentioning

confidence: 99%

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Spontaneous poisoning by Sida carpinifolia (Malvaceae) in horses

et al. 2017

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Sida carpinifolia poisoning causes a chronic neurodegenerative disorder associated with lysosomal storage by indolizidine alkaloids (swainsonine). The epidemiological, clinical, pathological and lectin histochemistry findings of an outbreak of natural poisoning by S. carpinifolia in horses in Rio Grande do Sul state, Brazil, are described. Five horses from a total of 15 that were kept on native pasture with large amounts of S. carpinifolia presented during 90 days clinical signs of progressive weight loss, incoordination, stiff gait and ramble, in addition to exacerbated reactions and locomotion difficulty after induced movement. Four horses died, and one of them was submitted for necropsy. At necropsy, no significant gross lesions were observed. Histological findings observed in the central nervous system were characterized by swollen neurons with cytoplasm containing multiple microvacuoles; these abnormalities were more severe in the thalamus, hippocampus, cerebellum and pons. Using lectin histochemistry, the pons and hippocampus sections stained positive for commercial lectin Con-A, sWGA and WGA. This study aimed to detail S. carpinifolia poisoning in horses to be included in the differential diagnoses of neurological diseases of horses.

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spontaneous poisoning by Sida carpinifolia (Malvaceae) in horses

et al. 2017

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“…Lectins WGA and sWGA characteristically mark GlcNAc residues (Monsingny et al 1980), and lectin Con-A binds speciϐically to α-mannose (Goldstein & Hayes 1978). Pattern seen on lectin-histochemistry here is similar to that observed previously in S. carpinifolia poisoned sheep, deer and cattle (Seitz et al 2005b, Pedroso et al 2009, Pedroso et al 2010, 2011.…”

Section: Discussionsupporting

confidence: 84%

Pathological findings in fetuses of goats and cattle poisoned by Sida carpinifolia (Malvaceae)

et al. 2012

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In Brazil, the consumption of Sida carpinifolia by livestock has been associated with neurological diseases linked to lysosomal storage disorders. This paper describes the pathological ϐindings in two caprine fetuses from dams that were experimentally poisoned with S. carpinifolia. The goats were orally dosed with 10 and 13g/kg of a paste of green chopped S. carpinifolia for 30 days and were observed for an additional 15 days period after the last dosage with the plant; thereafter they were euthanized and necropsied. The dams showed only slight clinical signs. The study also includes the ϐindings in one bovine fetus from a naturally S. carpinifolia poisoned cow which showed mild incoordination, generalized tremors, staggering, and frequent falls. The cow was euthanized and necropsied. While there were no signiϐicant histopathological changes in the goats, in the cow vacuolation of Purkinje neurons of the cerebellum, pancreatic acinar cells, and thyroid follicular cells were observed. The main microscopic changes observed in the caprine and bovine fetuses were vacuolation in the epithelium of renal tubules, thyroid follicular cells, and Purkinje neurons of the cerebellum. Transmission electron microscopy of sections from CNS of the cow and its fetus revealed vacuoles containing ϐine granular material surrounded by membrane. Lectin-histochemistry of CNS sections from goat fetuses marked lightly to sWGA lectins, WGA, and Con-A.INDEX TERMS: Poisonous plants, Swainsonine, Sida carpinifolia, Malvaceae, plant poisoning, lysosomal storage disease, pathological ϐindings, lectin-histochemistry, transmission electron microscopy, caprine and bovine fetuses.

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“…Ovinos parecem não ser afetados, e muitos criadores tem evitado o problema substituindo rebanhos caprinos por ovinos (Dantas et al 2007). Nas regiões Sul e Sudeste, esse grupo de plantas é representado por Sida carpinifolia, que causa intoxicações em caprinos, ovinos, bovinos e equinos (Colodel et al 2002, Loretti et al 2003, Pedroso et al 2010. Caracteristicamente, os animais que começam a se alimentar com plantas que contêm swainsonina, desenvolvem o hábito compulsivo de ingeri-las independentemente da palatabilidade e, mediante o mecanismo de facilitação social, induzem aos outros animais da mesma espécie a consumi-las (Barbosa et al 2006;Dantas et al 2007, Oliveira et al 2009, Pimentel et al 2012.…”

Section: Introductionunclassified