Abstract:Typhoid fever is one of the prevalent pandemics across many developing countries caused by Salmonella typhi. Vi capsular polysaccharide obtained from S. typhi has been licensed for use as a vaccine for children aged 2 years and above to prevent typhoid fever. Production and purification of Vi polysaccharide play a vital role in the immunogenicity and cost of vaccines. Four critical parameters, cetrimide concentration (% w/v), sodium acetate concentration (% w/v), alcohol concentration (% w/v), and operational … Show more
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