Dodine: A selective agent for certain soil fungi

Beilharz, Vyrna C.; Parbery, D. G.; Swart, H.J.

doi:10.1016/s0007-1536(82)80043-0

Cited by 67 publications

(22 citation statements)

References 7 publications

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“…The most common methods of determining levels of inoculum is based on isolation using selective media (Veen and Ferron 1966;Beilharz, Parberry, and Swart 1982;Baath 1991) and insect baiting methods (Zimmermann 1986). Selective media can be viewed as a semi-quantitative method.…”

Section: Selective Mediamentioning

confidence: 99%

Natural and released inoculum levels of entomopathogenic fungal biocontrol agents in soil in relation to risk assessment and in accordance with EU regulations

Scheepmaker

Butt

2010

Biocontrol Science and Technology

118

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Entomopathogenic fungi being developed as biological control agents (BCAs) may have the potential to spread and become established in the environment. For registration purposes, the risks concerning their persistence have to be evaluated according to EU legislation which requires the decline of BCAs to acceptable background levels unless related risks are acceptable. In order to deal with this requirement, applicants of a BCA need to give information on its persistence and natural background levels. For risk assessors and registration authorities guidance on how to evaluate data on natural background levels of indigenously occurring species (0same species as the introduced BCA) is needed. For this purpose, an overview is presented on background levels of some indigenous fungi as well as persistence data of some applied fungal BCAs. Data were restricted to commerical species. It was found that for the species Metarhizium anisopliae, Beauveria bassiana and Beauveria brongniartii natural densities were relatively low and introduced strains of these fungi decreased gradually in time. Many factors were found in the literature, such as intrinsic, edaphic, biotic, climatic, and cultural factors, that could explain this decline.

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Section: Selective Mediamentioning

confidence: 99%

Natural and released inoculum levels of entomopathogenic fungal biocontrol agents in soil in relation to risk assessment and in accordance with EU regulations

Scheepmaker

Butt

2010

Biocontrol Science and Technology

118

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“…EPF can be isolated either from the insect cadavers during natural epizootics or directly from soil from crop rhizosphere. The two most commonly used methods by researchers for soil isolation are either baiting the soil with an insect host, for example, Galleria mellonella (Zimmermann 1986) or using specific selective media (Veen and Ferron 1966; Doberski and Tribe 1980; Beilharz et al 1982). Since EPF are generally slow growers, other fast growing opportunistic fungi and bacteria dominate while culturing, which is addressed by the selective use of bacterial antibiotics and fungicides.…”

Section: Introductionmentioning

confidence: 99%

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

Teja

Rahman

2016

Mycology

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Entomopathogenic fungal species from the genera Beauveria, Metarhizium and Lecanicillium are important components in biological control of insect pests. However, temperature, humidity and UV radiation are among the important abiotic factors, which limit their effective usage. In this study, four local isolates of Metarhizium were isolated from different crop rhiospheres of Telangana and Andhra Pradesh states of India, identified and tested for their temperature tolerance in terms of radial growth, conidial yield per 10 mm disc and rate of conidial germination at different incubation temperatures. The results revealed that strains LaMa1 and MaAICRP performed well in terms of radial growth, conidial yield and rate of conidial germination, even at 35°C temperature. The role of such temperature tolerant strains in agriculture is discussed.

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“…In the development of selective media, dodine have been commonly used in several previous studies (Beilharz et al, 1982;Chase et al, 1986;Liu et al, 1993;Strasser et al, 1996;Rangel et al, 2010). Other studies have shown that fungicides such as thiabendazole, benomyl and cupric sulphate can also be used in selective media for isolating EPF from soils (Luz et al, 2007;Fernandes et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

The Occurrence of Entomopathogenic Fungi on Mineral and Peat Soils in Peninsular Malaysia

Kin¹,

Azmi²,

Kamarudin³

et al. 2017

American Journal of Agricultural and Biological Sciences

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The aims of this study are to isolate and identify Entomopathogenic Fungi (EPF) from mineral and peat soils in relation with the soil physico-chemical parameters. The mineral soil was sampled from the MPOB Research Station Hulu Paka in Terengganu, whereas peat soil was sampled from the MPOB Research Station Teluk Intan in Perak. Isolation of these fungi was carried out using a selective medium. Morphological characteristics of fungi were studied by observing the mycelium and conidia grown on agar plates using a light microscope. Soil physico-chemical parameters such as pH, water content, carbon and nitrogen content were also determined. Two species of EPF isolated from both types of soils were identified as Isaria amoenerosea and Metarhizium anisopliae. On potato dextrose agar, the colony of I. amoenerosea was pink in colour and slow growing with floccose mycelium which producing conidiophores with 3 to 4 phialides. The conidia were subglobose or irregular shapes between 2.0-3.0 µm long × 1.7-2.0 µm wide. The colony of M. anisopliae was whitish yellow and turned to dark green when matured; slow growing with floccose mycelium. The conidia were cylindrical with the dimension ranging from 6.0-7.0 µm long × 2.0-2.8 µm wide. The result shows that the occurrence of I. amoenerosea was more dominant than M. anisopliae. In mineral soil, out of 30 samples, I. amoenerosea was isolated from 25 soil samples (83%), while the M. anisopliae was only found in 15 samples (50%). In peat soil, out of 36 samples collected, 26 samples (72%) were found with I. amoenerosea, while the fungus M. anisopliae was isolated from eight samples (22%). In this study, the occurrence of EPF on mineral soil was higher than from peat soil, which was possibly due to low water content, high soil temperature and low C/N ratio.

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Dodine: A selective agent for certain soil fungi

Cited by 67 publications

References 7 publications

Natural and released inoculum levels of entomopathogenic fungal biocontrol agents in soil in relation to risk assessment and in accordance with EU regulations

Natural and released inoculum levels of entomopathogenic fungal biocontrol agents in soil in relation to risk assessment and in accordance with EU regulations

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

The Occurrence of Entomopathogenic Fungi on Mineral and Peat Soils in Peninsular Malaysia

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