Docosahexaenoic acid promotes neurogenesis in vitro and in vivo

Kawakita, Eisuke; Hashimoto, Michio; Shido, Osamu

doi:10.1016/j.neuroscience.2006.01.021

Cited by 367 publications

(276 citation statements)

References 36 publications

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“…This concentration is lower than typically applied to NSCs, where 1 µM DHA has been shown to induce differentiation (Katakura et al, 2009;Kawakita et al, 2006). However, our analysis showed that even at these low concentrations there was significant enrichment of the membrane phospholipids, consistent with NSCs incorporating and metabolizing PUFAs readily (Langelier et al, 2010).…”

Section: Discussionsupporting

confidence: 48%

“…Furthermore, the initial reports of cell viability assays showed cells were most viable when DHA was added at the lowest dose (0.1 µM) (Katakura et al, 2009). There are also differences in methodologies used, as in these other studies the fatty acids were dissolved in media containing 1.0% fattyacid free bovine serum albumin (Katakura et al, 2009;Kawakita et al, 2006), whereas in the present study DHA was dissolved in ethanol and then diluted into the media.. DHA inhibited NSC proliferation consistent with previous studies Katakura et al, 2009); however, others have reported DHA enhances NSC proliferation (Sakayori et al, 2011). Sakoyori and co-workers suggested that the difference in effects on proliferation was the due to the lower DHA concentration promoting the maintenance of gliogenic NSCs.…”

Section: Discussionmentioning

confidence: 99%

“…EPA and DHA enhance adult hippocampal neurogenesis in a variety of animal models (Dyall, 2011b) and DHA has consistently been shown to promote differentiation of neural stem cells (NSCs) (Katakura et al, 2009;Kawakita et al, 2006;Ma et al, 2010;Rashid et al, 2013). Elucidating the mechanisms behind these effects has been complicated by the convergent pathways involved in regulating neurogenesis and the pleiotropic effects of omega-3 PUFAs.…”

Section: Introductionmentioning

confidence: 99%

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Distinctive effects of eicosapentaenoic and docosahexaenoic acids in regulating neural stem cell fate are mediated via endocannabinoid signalling pathways

et al. 2016

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Running title: EPA, but not DHA induces proliferation in NSCs Abbreviations: AA, Arachidonic acid, AEA, anandamide, DHA. Docosahexaenoic acid; EPA, eicosapentaenoic acid, 2-AG, 2-arachidonylglycerol 2 Title: Distinctive effects of eicosapentaenoic and docosahexaenoic acids in regulating neural stem cell fate are mediated via endocannabinoid signalling pathways Emerging evidence suggests a complex interplay between the endocannabinoid system, omega-3 fatty acids and the immune system in the promotion of brain self-repair. However, it is unknown if all omega-3 fatty acids elicit similar effects on adult neurogenesis and if such effects are mediated or regulated by interactions with the endocannabinoid system. This study investigated the effects of DHA and EPA on neural stem cell (NSC) fate and the role of the endocannabinoid signalling pathways in these effects.EPA, but not DHA, significantly increased proliferation of NSCs compared to controls, an effect associated with enhanced levels of the endocannabinoid 2-arachidonylglycerol (2-AG) and p-p38 MAPK, effects attenuated by pre-treatment with CB1 (AM251) or CB2 (AM630) receptor antagonists. Furthermore, in NSCs derived from IL-1 deficient mice, EPA significantly decreased proliferation and p-p38 MAPK levels compared to controls, suggesting a key role for IL-1 signalling in the effects observed. Although DHA similarly increased 2-AG levels in wild-type NSCs, there was no concomitant increase in proliferation or p-p38 MAPK activity. In addition, in NSCs from IL-1 deficient mice, DHA significantly increased proliferation without effects on p-P38 MAPK, suggesting effects of DHA are mediated via alternative signalling pathways. These results provide crucial new insights into the divergent effects of EPA and DHA in regulating NSC proliferation and the pathways involved, and highlight the therapeutic potential of their interplay with endocannabinoid signalling in brain repair.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distinctive effects of eicosapentaenoic and docosahexaenoic acids in regulating neural stem cell fate are mediated via endocannabinoid signalling pathways

et al. 2016

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“…For instance, the number of BrdU-positive dividing cells is significantly higher in the hippocampus of fat-1 transgenic mice compared to WT littermates (He et al, 2009). The fat-1 mouse is a transgenic model rich in endogenous n −3 fatty acids; furthermore, similar results have also been reported with dietary supplementation (Kawakita et al, 2006) (Beltz et al, 2007) (Venna et al, 2009). The mechanisms by which n −3 PUFA exert modulatory effect on DG neurogenesis need to be identified.…”

Section: Discussionmentioning

confidence: 57%

n−3 fatty acids prevent impairment of neurogenesis and synaptic plasticity in B-cell activating factor (BAFF) transgenic mice

Crupi

Cambiaghi

Deckelbaum

et al. 2012

Preventive Medicine

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Objective. Autoimmune-prone B-cell activating factor transgenic mice, a mouse model of systemic lupus erythematosus and Sjögren's syndrome exhibit neuroinflammation, anxiety-like phenotype, deficit in adult hippocampal neurogenesis and impaired neurogenesis-dependent and neurogenesis-independent dentate gyrus long-term potentiation. Given that n−3 polyunsaturated fatty acids regulate hippocampal plasticity and inflammatory responses, we investigated whether n−3 polyunsaturated fatty acids-enriched diet might prevent age-dependent hippocampal changes in B-cell activating factor transgenic mice.Methods. B-cell activating factor transgenic mice were fed for 12 weeks with either n−3 polyunsaturated fatty acids-enriched or control diet and we tested the effect of this dietary supplementation on hippocampal inflammation, progenitor cell proliferation and neurogenesis-dependent and neurogenesis-independent long-term potentiation.Results. Dietary supplementation with n−-3 polyunsaturated fatty acids significantly decreased hippocampal microglial activation and increased the density of bromodeoxyuridine and doublecortin-positive newly-formed cells in the subventricular zone of hippocampus. Furthermore, B-cell activating factor transgenic mice fed with n-3 polyunsaturated fatty acids-enriched diet displayed normal long-term potentiation at the medial perforant pathway/dentate gyrus connections.Conclusions. The results indicate that n-3 fatty acids prevent neuroinflammation and deficits of hippocampal plasticity in B-cell activating factor transgenic mice and suggest that increased n-3 fatty acids intake might represent a potential therapeutic option to prevent neuropsychiatric symptoms associated with autoimmune diseases.

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“…However, neurons induced in response to supra-physiological levels of caffeine have a lower survival rate than control cells and increased proliferation does not yield an increase in AHN [121]. Curcumin is a natural phenolic component of yellow curry spice that increases AHN in rodents [53] and epidemiological studies have reported better cognitive performance from curry Mouse Increased survival [62], [61], [54], [8] Omega 3 fatty acids Rat Increased (DHA) [46] Flavonoids Rat, chronically stressed Increased proliferation [3] Blueberry Rat Increased proliferation [12] Curcumin low concentrations Mouse Increased proliferation [53] Retinoic acid excess Mouse Decreased proliferation [16] Vitamin A deficiency Rat Decreased proliferation (rescued with retinoic acid) [9] Thiamine deficiency Mouse Decreased proliferation/survival [128] Zinc deficiency Rat male Decreased proliferation/survival [14] Folate consumption in ageing populations [83]. Moreover, in vitro studies have shown that curcumin exerted biphasic effects on progenitor cells; low concentrations stimulated cell proliferation, whereas high concentrations were cytotoxic.…”

Section: Dietary Modulation Of Adult Hippocampal Neurogenesismentioning

confidence: 99%

Impact of diet on adult hippocampal neurogenesis

2009

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Research over the last 5 years has firmly established that learning and memory abilities, as well as mood, can be influenced by diet, although the mechanisms by which diet modulates mental health are not well understood. One of the brain structures associated with learning and memory, as well as mood, is the hippocampus. Interestingly, the hippocampus is one of the two structures in the adult brain where the formation of newborn neurons, or neurogenesis, persists. The level of neurogenesis in the adult hippocampus has been linked directly to cognition and mood. Therefore, modulation of adult hippocampal neurogenesis (AHN) by diet emerges as a possible mechanism by which nutrition impacts on mental health. In this study, we give an overview of the mechanisms and functional implications of AHN and summarize recent findings regarding the modulation of AHN by diet.

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Docosahexaenoic acid promotes neurogenesis in vitro and in vivo

Cited by 367 publications

References 36 publications

Distinctive effects of eicosapentaenoic and docosahexaenoic acids in regulating neural stem cell fate are mediated via endocannabinoid signalling pathways

Distinctive effects of eicosapentaenoic and docosahexaenoic acids in regulating neural stem cell fate are mediated via endocannabinoid signalling pathways

n−3 fatty acids prevent impairment of neurogenesis and synaptic plasticity in B-cell activating factor (BAFF) transgenic mice

Impact of diet on adult hippocampal neurogenesis

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