Docosahexaenoic acid inhibits the invasion of MDA-MB-231 breast cancer cells through upregulation of cytokeratin-1

Abstract

Abstract. Docosahexaenoic acid (DHa), the main member of the omega-3 essential fatty acid family, has been shown to reduce the invasion of the triple-negative breast cancer cell line MDa-MB-231, but the mechanism involved remains unclear. In the present study, a proteomic approach was used to define changes in protein expression induced by DHa. Proteins from crude membrane preparations of MDa-MB-231 cells treated with 100 µM DHa were separated by two-dimensional electrophoresis (2-De) and differentially expressed proteins were identified using MALDI-TOF mass spectrometry. The main changes observed were the upregulation of Keratin, type Ⅱ cytoskeletal 1 (KRT1), catalase and lamin-A/C. immunocytochemistry analyses confirmed the increase in Krt1 induced by DHa. furthermore, in vitro invasion assays showed that sirna against Krt1 was able to reverse the DHa-induced inhibition of breast cancer cell invasion. in conclusion, Krt1 is involved in the anti-invasive activity of DHa in breast cancer cells.

Serum proteins differentially expressed in early- and late-onset preeclampsia assessed using iTRAQ proteomics and bioinformatics analyses

“…GRN, CD5L, ENO1, CHL1, CRISP3, VASN, and TNIK promote the invasive ability of tumor cells (Aran et al, 2018;Bhandari et al, 2019;Buhusi et al, 2003;Chon et al, 2016;Song et al, 2014;Voshtani et al, 2019;Wang et al, 2019). By contrast, SEMA4B, KRT1, KRT9, FBLN1, and PTGDS are involved in the anti-invasive activity of tumor cells (Blanckaert et al, 2015;Jian et al, 2015;Marano et al, 2018;Zhang et al, 2018). MMP2 plays an important role in trophoblast invasion and is generally thought to be downregulated in PE (Wu et al, 2016).…”

DHA induces apoptosis of human malignant breast cancer tissues by the TLR‑4/PPAR‑α pathways

“…Dietary unsaturated fatty acids, particularly DHA, are considered to serve an important role in reducing the risk of developing BC ( 4 ). Certain evidence has demonstrated that DHA oil is beneficial for inhibiting mammary gland carcinogenesis ( 5 – 10 ). The mechanisms of DHA oil on the inhibition of tumor proliferation and the promotion of apoptosis are complex ( 5 – 10 ).…”

“…Certain evidence has demonstrated that DHA oil is beneficial for inhibiting mammary gland carcinogenesis ( 5 – 10 ). The mechanisms of DHA oil on the inhibition of tumor proliferation and the promotion of apoptosis are complex ( 5 – 10 ). Among the numerous key factors, oxidative stress serves an important role in the initiation, promotion, progression and apoptosis of BC by interfering with the intracellular signal transduction pathways, and inducing DNA damage ( 11 ).…”

Do Olive and Fish Oils of the Mediterranean Diet Have a Role in Triple Negative Breast Cancer Prevention and Therapy? An Exploration of Evidence in Cells and Animal Models

“…Activating the EMT and progressing through the metastatic process is a key determinant of BC prognosis as the median survival rate for metastatic TNBC is ~10–13 mo ( 5 ). In addition to proliferation, DHA was shown to inhibit migration ( 44 , 49 , 51 , 52 ) and invasion ( 41 , 44 , 50 ) of TNBC in culture. Mouse experiments using the tail vein injection model in Fat-1 mice, which produce higher amounts of endogenous ω-3 essential fatty acids, also suggested a potential anti-metastatic effect ( 41 ).…”

“…Treatment with DHA (100 μM for 24 h) also decreased invasion of MDA-MB-231 cells in Boyden chamber assays by 60% compared to control cells ( 50 ). These effects were abrogated upon co-treatment with siRNA against keratin, type II cytoskeletal 1 (KRT1), which was significantly upregulated upon treatment with DHA alone.…”