Docosahexaenoic acid-containing choline phospholipid modulates LPS-induced neuroinflammation in vivo and in microglia in vitro

Fourrier, Célia; Remus-Borel, Julie; Greenhalgh, Andrew D.; Guichardant, Michel; Bernoud-Hubac, Nathalie; Lagarde, Michel; Joffre, Corinne; Layé, Sophie

doi:10.1186/s12974-017-0939-x

Cited by 92 publications

(62 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, cytokines (especially HMGB1, NFκB, and TNFα [30]) secreted from circulating or fixed mononuclear cells regulated microglial feedback reactions [31], upregulating MCP-1 expression and inflammatory cascade signaling pathways and triggering neuroinflammation in the hippocampus [32]. In addition, microglial elimination or inhibition suppressed hippocampal inflammatory responses and reduced the levels of cytokines such as IL-6, IL-1β, and TNFα following anesthesia and other pathological conditions [17,33,34]. Moreover, the level of inositol 1,4,5-triphosphate type 2 receptor-dependent calcium was reduced in astrocytes, even after a sedative dose of anesthetic [35], and long-term inhibition of astrocytes is consistent with synaptic suppression and cognitive deficiencies [24].…”

Section: Discussionmentioning

confidence: 99%

Hippocampal microglial activation triggers a neurotoxic-specific astrocyte response and mediates etomidate-induced long-term synaptic inhibition

Chen

Meng

et al. 2020

J Neuroinflammation

View full text Add to dashboard Cite

Background: Accumulating evidence has highlighted the importance of microglial and astrocyte responses in the pathological development of postoperative cognitive dysfunction (POCD). However, the mechanisms involved are not well understood. Methods: A perioperative neurocognitive disorders (PND) mouse model was generated by administering etomidate, and cognitive function was assessed using the Morris water maze and novel object recognition tests. Excitatory and inhibitory postsynaptic currents were recorded to analyze neuronal activity. In addition, microglia and astrocytes were isolated by magnetic-activated cell sorting, and genes that were activated in these cells were identified using quantitative polymerase chain reaction. Results: We observed dramatic cognitive impairment at 1 and 3 weeks after etomidate was administered to 18 month-old mice. Microglia and astrocytes isolated from the hippocampus showed significant microglial activation during the early pathological stage (i.e., 1 week after etomidate injection) and an A1-specific astrocyte response during the late pathological stage (i.e., 3 weeks after etomidate injection). Furthermore, when microglia were eliminated before etomidate was injected, the A1-specific astrocyte activation response was significantly reduced, and cognitive function improved. However, when microglia were eliminated after etomidate application, astrocyte activation and cognitive function were not significantly altered. In addition, activating microglia immediately after a sedative dose of etomidate was injected markedly increased A1-specific astrocyte activation and cognitive dysfunction. Conclusions: A1-specific astrocyte activation is triggered by activated microglia during the initial pathological stage of PND and induces long-term synaptic inhibition and cognitive deficiencies. These results improve our understanding of how PND develops and may suggest therapeutic targets.

show abstract

Section: Discussionmentioning

confidence: 99%

Hippocampal microglial activation triggers a neurotoxic-specific astrocyte response and mediates etomidate-induced long-term synaptic inhibition

Chen

Meng

et al. 2020

J Neuroinflammation

View full text Add to dashboard Cite

show abstract

“…Long-chain polyunsaturated fatty acids such as omega-3 (ω-3) have been widely investigated for their ability to alter behavior and the immune system [146]. ω-3 fatty acids can reduce microglial activation and the release of pro-inflammatory cytokines [146][147][148][149][150]. In addition to their effects on the immune system, supplementation of ω-3 fatty acids in adulthood can reduce anxiety-like and depression-like behaviors following adolescent LPS injections [151].…”

Section: Early Preventative Interventionsmentioning

confidence: 99%

Microglial Function in the Effects of Early-Life Stress on Brain and Behavioral Development

Catale

Gironda

Iacono

et al. 2020

JCM

View full text Add to dashboard Cite

The putative effects of early-life stress (ELS) on later behavior and neurobiology have been widely investigated. Recently, microglia have been implicated in mediating some of the effects of ELS on behavior. In this review, findings from preclinical and clinical literature with a specific focus on microglial alterations induced by the exposure to ELS (i.e., exposure to behavioral stressors or environmental agents and infection) are summarized. These studies were utilized to interpret changes in developmental trajectories based on the time at which the stress occurred, as well as the paradigm used. ELS and microglial alterations were found to be associated with a wide array of deficits including cognitive performance, memory, reward processing, and processing of social stimuli. Four general conclusions emerged: (1) ELS interferes with microglial developmental programs, including their proliferation and death and their phagocytic activity; (2) this can affect neuronal and non-neuronal developmental processes, which are dynamic during development and for which microglial activity is instrumental; (3) the effects are extremely dependent on the time point at which the investigation is carried out; and (4) both pre- and postnatal ELS can prime microglial reactivity, indicating a long-lasting alteration, which has been implicated in behavioral abnormalities later in life.

show abstract

“…Analyses of the physiological functions of n‐3 PUFAs have been conducted via their triacylglycerols or ethyl esters . Recently, EPA and DHA enriched PLs from marine resources such as krill oil have drawn attention for their potential health benefits . The main members of the PL class of marine products are PC and phosphatidylethanolamine (PE), but not PG.…”

Section: Introductionmentioning

confidence: 99%

Preparation of n‐3 Polyunsaturated Phosphatidylglycerol from Salmon Roe Lipids by Phospholipase D and In Vitro Digestion

Chen

Beppu

Miyashita

et al. 2019

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

Phosphatidylglycerol (PG) is a highly functional phospholipid (PL), which has many physiological functions. However, naturally occurring PG binding n‐3 polyunsaturated fatty acid (n‐3 PUFA) is low in content, resulting in a scarcity of industrial bio‐resources of n‐3 PUFA enriched PG. The current study investigates the preparation of salmon roe PG (SRPG) from three types of salmon roe lipids and glycerol via phospholipase D (PLD)‐mediated transphosphatidylation. The yields of SRPG obtained from salmon roe total lipid (SRTL) and salmon roe PL (SRPL) are higher than those obtained from purified salmon roe phosphatidylcholine (SRPC) in aqueous system. Following a 24 h reaction with 0.75 U PLD, SRTL, and SRPL yield up to 96.4 mol% and 96.7 mol% SRPG, respectively. In addition, more fatty acids are released from synthesized SRPG via hydrolysis by pancreatic enzymes than from SRPC and soybean PC in in vitro digestion model. Fatty acids at the sn‐2 position of SRPG are completely liberated by 0.04 U of phospholipase A2 (PLA2) during a 6 h reaction, whereas fatty acids of SRPC are partially unhydrolyzed even after a 24 h reaction. Our results suggest that SRPG converted from salmon lipids by PLD is a functional PL with high bioavailability of n‐3 PUFAs. Practical Applications: Phosphatidylglycerol rich in n‐3 PUFAs is prepared from salmon roe lipids (SRPG) catalyzed by PLD. The SRPG yields reach 96.4 mol% and 96.7 mol% of phosphatidylcholine contained in SRTL and SRPL, respectively, in aqueous reaction system. Fatty acids rich in n‐3 PUFAs at sn‐2 position of prepared SRPG are rapidly liberated by PLA2 in an in vitro digestion model.

show abstract

Docosahexaenoic acid-containing choline phospholipid modulates LPS-induced neuroinflammation in vivo and in microglia in vitro

Cited by 92 publications

References 68 publications

Hippocampal microglial activation triggers a neurotoxic-specific astrocyte response and mediates etomidate-induced long-term synaptic inhibition

Hippocampal microglial activation triggers a neurotoxic-specific astrocyte response and mediates etomidate-induced long-term synaptic inhibition

Microglial Function in the Effects of Early-Life Stress on Brain and Behavioral Development

Preparation of n‐3 Polyunsaturated Phosphatidylglycerol from Salmon Roe Lipids by Phospholipase D and In Vitro Digestion

Contact Info

Product

Resources

About