Docosahexaenoic acid attenuates in endocannabinoid synthesis in RAW 264.7 macrophages activated with benzo(a)pyrene and lipopolysaccharide

Gdula‐Argasińska, Joanna; Bystrowska, Beata

doi:10.1016/j.toxlet.2016.06.017

Cited by 16 publications

(21 citation statements)

References 37 publications

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“…An endogenous tryptophan metabolite, for example 5-methoxytryptophan (5-MTP), controls of COX-2 overexpression (Wu et al, 2014). The anti-inflammatory and pro-resolving properties of the n-3 fatty acids were observed in our previous studies (Gdula-Argasińska et al, 2015a;Gdula-Argasińska et al, 2015b;Gdula-Argasińska et al, 2016a;Gdula-Argasińska et al, 2016b). In Park and coworkers study (2014), ethyl linoleate, which is isolated from the cloves of A. sativum, down-regulated the inducible nitric oxide synthase (iNOS) and COX-2 expression, thereby reducing the production of nitric oxide and prostaglandin E2 in the LPS-activated RAW 264.7 cells.…”

Section: Resultsmentioning

confidence: 90%

“…1H). AhR, as a nuclear factor, participates in the transcription factor κB (NF-κB) signaling pathways regulating inflammation, immune responses, and other functions (Nguyen et al, 2013;Gdula-Argasińska et al, 2015b;Gdula-Argasińska et al, 2016a;Gdula-Argasińska et al, 2016b). Accumulating evidence suggests close interactions between AhR and NF-ĸB pathways, and these interactions are potentially important mechanisms for many pathological processes, such as carcinogenesis and alteration of xenobiotic metabolism (Nguyen et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

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Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

et al. 2017

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The purpose of this study was to analyze the indolic, phenolic, and fatty acid content and antioxidant activity of garlic sprouts growing in the dark and in the daylight. The pro- or anti-inflammatory properties of the garlic sprout extract were investigated by evaluating the cyclooxygenase-2 (COX-2), prostaglandin E synthase (cPGES), glutathione S transferase (GSTM1), nuclear factor NF-κB, peroxisome proliferator-activated receptors (PPARs), and aryl hydrocarbon receptor (AhR) protein levels in the RAW 264.7 cells activated with lipopolysaccharide (LPS). The highest amount of total indolic (73.56 mg/100 g f.w.) and phenolic compounds (36.23 mg/100 g f.w.) was detected in garlic sprouts grown in the daylight. Studies on antioxidant activity (the FRAP and DPPH method) of garlic sprouts showed that this activity is significantly higher for sprouts grown in full access to light when compared to those grown in the dark. In garlic sprout extracts, α-linolenic acid (ALA) was found to be in greater amount. COX-2 and cPGES level was lower when compared to LPS alone activated cells. After garlic extract treatment, higher level of GSTM1, PPARΥ, cytosolic p50 and p65 protein, as well as a lower NF-ĸB p50/p65 activity was noted in the RAW 264.7 cells which suggested PPARs and AhR transrepression mechanism of NF-ĸB signalling. The obtained results indicate Allium sativum sprouts are a rich source of n-3 fatty acids, indolic and phenolic compounds characterized by anti-inflammatory and antioxidative activity, which may support their high therapeutic and dietary potential.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

et al. 2017

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“…The synthesis of fatty acid methyl esters (FAME) was carried out with 14% BF 3 in methanol. FAME were analyzed using gas chromatography (Agilent 6890 N, Agilent Inc., Santa Clara, CA, USA) with a J&W DB-23 (60 m, ID 0.25 mm, 0.25 μm) column, as described earlier (Gdula-Argasińska et al 2016 ; Gdula-Argasińska and Bystrowska 2016 ). FAME were identified according to standards (Sigma–Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Fatty acids and selected endocannabinoids content in cerebrospinal fluids from patients with neuroinfections

et al. 2018

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Neuroinfections are a significant medical problem and can have serious health consequences for patients. Their outcome, if not fatal, can be associated with permanent residual deficits. Cerebrospinal fluid (CSF) examination is commonly used for meningitis confirmation. Fatty acids (FA) are precursors of lipid mediators with pharmacological activity. They actively modulate inflammation as well as contribute to its resolution. Therefore the aim of this study was to determine the FA and selected endocannabinoids (ECB) content in the CSF obtained from patients with bacterial (BM) and viral meningitis (VM) using chromatographic techniques. A significantly lower level of saturated FA was found in patients with BM and VM as compared to controls. There was a significantly higher concentration of long-chain monounsaturated FA and polyunsaturated n-6 FA in the CSF obtained from patients with neuroinfection. Moreover, a significant reduction of n-3 FA in CSF obtained from patients with BM and VM was demonstrated. The highest amount of ECB was detected in the CSF of patients with VM: eicosapentaenoyl ethanolamide (1.65 pg/mL), docosahexaenoyl ethanolamide (655.5 pg/mL) and nervonoyl ethanolamide (3.09 ng/mL). Results indicate the participation of long-chain monounsaturated and polyunsaturated FA and their derivatives in the inflammatory process and likely in the process of resolution of inflammation during neuroinfection. It seems that the determination of the FA and ECB profile in CSF may be a valuable biomarker of health and may allow the development of new pharmacological strategies, therapeutic goals and fatty acids supplementation necessary in the fight against inflammation of the central nervous system.

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“…Apart from the studies aiming at analyzing the influence of individual products with a specific content on the cascade of changes induced by BaP, there are many papers describing the influence of isolated single compounds, such as vitamin C, β-carotenoids, retinol, retinoic acid, phenethyl isothiocyanate, luteolin, chrysin, naringenin, quercetin, damnacanthal, proanthocyanidine, resveratrol, berberine, catechin, anthocyanins, apigenin or kaempferol. The mechanisms of action of the selected phytochemicals and the above-mentioned dietary compounds are presented in Table 1 [52][53][54][55][56][57][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73].…”

Section: Benzo(a)pyrene (Bap)mentioning

confidence: 99%

“…Zhou et al [55] showed that dietary fish oil played a protective role in BaP-induced hepatic carcinogenesis and significantly reduced the DNA adducts level in the mice model [55]. In the case of in vitro studies, clear anti-inflammatory properties of docosahexaenoic acid under exposure to BaP were shown [56]. Docosahexaenoic acid treatment increased BaP membrane accumulation connected with the induction of phases I and II metabolizing and detoxifying enzymes, as well as reduction of DNA adducts and BaP metabolites in the supplemented cells [57].…”

Section: Benzo(a)pyrene (Bap)mentioning

confidence: 99%

Health risk in transport workers. Part II. Dietary compounds as modulators of occupational exposure to chemicals

Kozłowska

Gromadzińska

Wąsowicz

2019

Int J Occup Med Environ Health

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Professional drivers are exposed to a number of factors that have a negative influence on their health status. These include vibrations, noise, the lack of fresh air in the car cabin, shift work (frequently at night), monotony resulting from permanent repetition of certain actions, static loads due to immobilization in a sitting position, stress resulting from the need to ensure safety in heavy traffic, as well as air pollution (dust, volatile organic substances, nitrogen and sulfur oxides, polycyclic aromatic hydrocarbons, heavy metals, dioxins, furans and others). Factors associated with the specificity of the profession of a driver, including exposure to chemical substances, result in an increased risk of the development of many diseases, i.e., obesity, diabetes, heart disease, hypertension, extensive genitourinary pathology experienced by taxi drivers, lung cancer and other forms of cancer. In the case of drivers, especially those covering long distances, there are also actual difficulties related to ensuring a proper diet. Although attempts at interventional research that would change the principles of nutrition, as well as ensure physical activity and weight reduction, have been made, their results have not been satisfactory. The paper focuses on the discussion on the role of a diet and dietary phytochemicals in the prevention of adverse health effects of such chemicals as a mix of chemicals in the polluted air, benzo(a)pyrene, benzene and metals (lead, cadmium, chromium, nickel), which are the main sources of exposure in the case of transport workers. Int J Occup Med Environ Health. 2019;32(4):441 -64

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Docosahexaenoic acid attenuates in endocannabinoid synthesis in RAW 264.7 macrophages activated with benzo(a)pyrene and lipopolysaccharide

Cited by 16 publications

References 37 publications

Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

Fatty acids and selected endocannabinoids content in cerebrospinal fluids from patients with neuroinfections

Health risk in transport workers. Part II. Dietary compounds as modulators of occupational exposure to chemicals

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