Docosahexaenoic acid and etanercept could reduce functional and metabolic alterations during collagen-induced arthritis in rats without any synergistic effect

Leger, Thibault; Brun, Aurelien; Lanchais, Kassandra; Rigaudière, Jean‐Paul; Briat, Arnaud; Guitton, Yann; Marchand, Fabien; Tournadre, Anne; Capel, Frédéric

doi:10.1016/j.lfs.2023.121826

Cited by 1 publication

(1 citation statement)

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“…In recent years, the diverse benefits of DHA for humans and other organisms have been extensively studied [35][36][37]. Our previous investigation showed DHA inhibited TNF-α-induced osteoclast formation and bone destruction in vivo and in vitro via GPR120 [13].…”

Section: Discussionmentioning

confidence: 99%

Generating Bone Marrow Chimeric Mouse Using GPR120 Deficient Mouse for the Study of DHA Inhibitory Effect on Osteoclast Formation and Bone Resorption

Ma,

Kitaura,

Ohori

et al. 2023

IJMS

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Docosahexaenoic acid (DHA) is an omega-3 fatty acid that exerts physiological effects via G protein-coupled receptor 120 (GPR120). In our previous studies, we figured out the inhibitory effects of DHA on TNF-α (Tumor necrosis factor-α)-induced osteoclastogenesis via GPR120 in vivo. Moreover, DHA directly suppressed RANKL expression in osteoblasts via GPR120 in vitro. In this study, we generated bone marrow chimeric mice using GPR120 deficient mice (GPR120-KO) to study the inhibitory effects of DHA on bone resorption and osteoclast formation. Bone marrow cells of wild-type (WT) or GPR120-KO mice were transplanted into irradiated recipient mice, which were WT or GPR120 deficient mice. The resulting chimeric mice contained stromal cells from the recipient and bone marrow cells, including osteoclast precursors, from the donor. These chimeric mice were used to perform a series of histological and microfocus computed tomography (micro-CT) analyses after TNF-α injection for induction of osteoclast formation with or without DHA. Osteoclast number and bone resorption were found to be significantly increased in chimeric mice, which did not express GPR120 in stromal cells, compared to chimeric mice, which expressed GPR120 in stromal cells. DHA was also found to suppress specific signaling pathways. We summarized that DHA suppressed TNF-α-induced stromal-dependent osteoclast formation and bone resorption via GPR120.

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